Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.

To prevent progression to AIDS, persons infected with human immunodeficiency virus type 1 (HIV-1) must remain on highly active antiretroviral therapy (HAART) indefinitely since this modality does not eradicate the virus. The mechanisms involved in viral persistence during HAART are poorly understood...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jesse D Deere, Joanne Higgins, Elda Cannavo, Andradi Villalobos, Lourdes Adamson, Emilie Fromentin, Raymond F Schinazi, Paul A Luciw, Thomas W North
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2eec88abdca4b5fb5fd4613777254fa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a2eec88abdca4b5fb5fd4613777254fa
record_format	dspace
spelling	oai:doaj.org-article:a2eec88abdca4b5fb5fd4613777254fa2021-12-02T20:19:50ZViral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.1932-620310.1371/journal.pone.0011640https://doaj.org/article/a2eec88abdca4b5fb5fd4613777254fa2010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20668516/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203To prevent progression to AIDS, persons infected with human immunodeficiency virus type 1 (HIV-1) must remain on highly active antiretroviral therapy (HAART) indefinitely since this modality does not eradicate the virus. The mechanisms involved in viral persistence during HAART are poorly understood, but an animal model of HAART could help elucidate these mechanisms and enable studies of HIV-1 eradication strategies. Due to the specificity of non-nucleoside reverse transcriptase (RT) inhibitors (NNRTIs) for HIV-1, we have used RT-SHIV, a chimeric virus of simian immunodeficiency virus with RT from HIV-1. This virus is susceptible to NNRTIs and causes an AIDS-like disease in rhesus macaques. In this study, two groups of HAART-treated, RT-SHIV-infected macaques were analyzed to determine viral decay kinetics. In the first group, viral loads were monitored with a standard TaqMan RT-PCR assay with a limit of detection of 50 viral RNA copies per mL. Upon initiation of HAART, viremia decayed in a bi-phasic manner with half-lives of 1.7 and 8.5 days, respectively. A third phase was observed with little further decay. In the second group, the macaques were followed longitudinally with a more sensitive assay utilizing ultracentrifugation to concentrate virus from plasma. Bi-phasic decay of viral RNA was also observed in these animals with half-lives of 1.8 and 5.8 days. Viral loads in these animals during a third phase ranged from 2-58 RNA copies/mL, with little decay over time. The viral decay kinetics observed in these macaques are similar to those reported for HIV-1 infected humans. These results demonstrate that low-level viremia persists in RT-SHIV-infected macaques despite a HAART regimen commonly used in humans.Jesse D DeereJoanne HigginsElda CannavoAndradi VillalobosLourdes AdamsonEmilie FromentinRaymond F SchinaziPaul A LuciwThomas W NorthPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11640 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jesse D Deere Joanne Higgins Elda Cannavo Andradi Villalobos Lourdes Adamson Emilie Fromentin Raymond F Schinazi Paul A Luciw Thomas W North Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.
description	To prevent progression to AIDS, persons infected with human immunodeficiency virus type 1 (HIV-1) must remain on highly active antiretroviral therapy (HAART) indefinitely since this modality does not eradicate the virus. The mechanisms involved in viral persistence during HAART are poorly understood, but an animal model of HAART could help elucidate these mechanisms and enable studies of HIV-1 eradication strategies. Due to the specificity of non-nucleoside reverse transcriptase (RT) inhibitors (NNRTIs) for HIV-1, we have used RT-SHIV, a chimeric virus of simian immunodeficiency virus with RT from HIV-1. This virus is susceptible to NNRTIs and causes an AIDS-like disease in rhesus macaques. In this study, two groups of HAART-treated, RT-SHIV-infected macaques were analyzed to determine viral decay kinetics. In the first group, viral loads were monitored with a standard TaqMan RT-PCR assay with a limit of detection of 50 viral RNA copies per mL. Upon initiation of HAART, viremia decayed in a bi-phasic manner with half-lives of 1.7 and 8.5 days, respectively. A third phase was observed with little further decay. In the second group, the macaques were followed longitudinally with a more sensitive assay utilizing ultracentrifugation to concentrate virus from plasma. Bi-phasic decay of viral RNA was also observed in these animals with half-lives of 1.8 and 5.8 days. Viral loads in these animals during a third phase ranged from 2-58 RNA copies/mL, with little decay over time. The viral decay kinetics observed in these macaques are similar to those reported for HIV-1 infected humans. These results demonstrate that low-level viremia persists in RT-SHIV-infected macaques despite a HAART regimen commonly used in humans.
format	article
author	Jesse D Deere Joanne Higgins Elda Cannavo Andradi Villalobos Lourdes Adamson Emilie Fromentin Raymond F Schinazi Paul A Luciw Thomas W North
author_facet	Jesse D Deere Joanne Higgins Elda Cannavo Andradi Villalobos Lourdes Adamson Emilie Fromentin Raymond F Schinazi Paul A Luciw Thomas W North
author_sort	Jesse D Deere
title	Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.
title_short	Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.
title_full	Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.
title_fullStr	Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.
title_full_unstemmed	Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.
title_sort	viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of aids.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/a2eec88abdca4b5fb5fd4613777254fa
work_keys_str_mv	AT jesseddeere viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids AT joannehiggins viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids AT eldacannavo viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids AT andradivillalobos viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids AT lourdesadamson viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids AT emiliefromentin viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids AT raymondfschinazi viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids AT paulaluciw viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids AT thomaswnorth viraldecaykineticsinthehighlyactiveantiretroviraltherapytreatedrhesusmacaquemodelofaids
_version_	1718374181325766656

Viral decay kinetics in the highly active antiretroviral therapy-treated rhesus macaque model of AIDS.

Ejemplares similares