Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets

Abstract Graphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatel...

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Autores principales:	Maria Suarez-Diez, Santiago Porras, Felix Laguna-Teno, Peter J. Schaap, Juan A. Tamayo-Ramos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2f46e4ef1184eccaa825c206f7b039d
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spelling	oai:doaj.org-article:a2f46e4ef1184eccaa825c206f7b039d2021-12-02T14:28:22ZToxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets10.1038/s41598-020-60101-72045-2322https://doaj.org/article/a2f46e4ef1184eccaa825c206f7b039d2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-60101-7https://doaj.org/toc/2045-2322Abstract Graphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatelets (GN) on the unicellular fungal model Saccharomyces cerevisiae was performed. While cell proliferation was not negatively affected even in the presence of 800 mg L−1 of the nanomaterial for 24 hours, oxidative stress was induced at a lower concentration (160 mg L−1), after short exposure periods (2 and 4 hours). No DNA damage was observed under a comet assay analysis under the studied conditions. In addition, to pinpoint the molecular mechanisms behind the early oxidative damage induced by GN and to identify possible toxicity pathways, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L−1 of GN was studied. Both GN concentrations induced expression changes in a common group of genes (337), many of them related to the fungal response to reduce the nanoparticles toxicity and to maintain cell homeostasis. Also, a high number of genes were only differentially expressed in the GN800 condition (3254), indicating that high GN concentrations can induce severe changes in the physiological state of the yeast.Maria Suarez-DiezSantiago PorrasFelix Laguna-TenoPeter J. SchaapJuan A. Tamayo-RamosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Suarez-Diez Santiago Porras Felix Laguna-Teno Peter J. Schaap Juan A. Tamayo-Ramos Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
description	Abstract Graphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatelets (GN) on the unicellular fungal model Saccharomyces cerevisiae was performed. While cell proliferation was not negatively affected even in the presence of 800 mg L−1 of the nanomaterial for 24 hours, oxidative stress was induced at a lower concentration (160 mg L−1), after short exposure periods (2 and 4 hours). No DNA damage was observed under a comet assay analysis under the studied conditions. In addition, to pinpoint the molecular mechanisms behind the early oxidative damage induced by GN and to identify possible toxicity pathways, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L−1 of GN was studied. Both GN concentrations induced expression changes in a common group of genes (337), many of them related to the fungal response to reduce the nanoparticles toxicity and to maintain cell homeostasis. Also, a high number of genes were only differentially expressed in the GN800 condition (3254), indicating that high GN concentrations can induce severe changes in the physiological state of the yeast.
format	article
author	Maria Suarez-Diez Santiago Porras Felix Laguna-Teno Peter J. Schaap Juan A. Tamayo-Ramos
author_facet	Maria Suarez-Diez Santiago Porras Felix Laguna-Teno Peter J. Schaap Juan A. Tamayo-Ramos
author_sort	Maria Suarez-Diez
title	Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
title_short	Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
title_full	Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
title_fullStr	Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
title_full_unstemmed	Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
title_sort	toxicological response of the model fungus saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a2f46e4ef1184eccaa825c206f7b039d
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Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets

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