Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Qinmei Wang,1 Qiong Wang,2 Wei Teng2 1Laboratory of Biomaterials, Key Laboratory on Assisted Circulation, Ministry of Health, Cardiovascular Division, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Department of Prosthodontics, Hospital of Stoma...

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Autores principales:	Wang QM, Wang Q, Teng W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Injectability Electroactivity Nanocomposite hydrogels Reinforcement Tetraaniline Alginate Gelatin Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a2f6021d71e94ddfa678a3c8da47ebe7
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spelling	oai:doaj.org-article:a2f6021d71e94ddfa678a3c8da47ebe72021-12-02T02:42:21ZInjectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications1178-2013https://doaj.org/article/a2f6021d71e94ddfa678a3c8da47ebe72016-01-01T00:00:00Zhttps://www.dovepress.com/injectable-degradable-electroactive-nanocomposite-hydrogels-containing-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qinmei Wang,1 Qiong Wang,2 Wei Teng2 1Laboratory of Biomaterials, Key Laboratory on Assisted Circulation, Ministry of Health, Cardiovascular Division, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Department of Prosthodontics, Hospital of Stomatology, Institute of Stomatological Research, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, People’s Republic of China Abstract: Injectable electroactive hydrogels (eGels) are promising in regenerative medicine and drug delivery, however, it is still a challenge to obtain such hydrogels simultaneously possessing other properties including uniform structure, degradability, robustness, and biocompatibility. An emerging strategy to endow hydrogels with desirable properties is to incorporate functional nanoparticles in their network. Herein, we report the synthesis and characterization of an injectable hydrogel based on oxidized alginate (OA) crosslinking gelatin reinforced by electroactive tetraaniline-graft-OA nanoparticles (nEOAs), where nEOAs are expected to impart electroactivity besides reinforcement without significantly degrading the other properties of hydrogels. Assays of transmission electron microscopy, 1H nuclear magnetic resonance, and dynamic light scattering reveal that EOA can spontaneously and quickly self-assemble into robust nanoparticles in water, and this nanoparticle structure can be kept at pH 3~9. Measurement of the gel time by rheometer and the stir bar method confirms the formation of the eGels, and their gel time is dependent on the weight content of nEOAs. As expected, adding nEOAs to hydrogels does not cause the phase separation (scanning electron microscopy observation), but it improves mechanical strength up to ~8 kPa and conductivity up to ~10-6 S/cm in our studied range. Incubating eGels in phosphate-buffered saline leads to their further swelling with an increase of water content <6% and gradual degradation. When growing mesenchymal stem cells on eGels with nEOA content ≤14%, the growth curves and morphology of cells were found to be similar to that on tissue culture plastic; when implanting these eGels on a chick chorioallantoic membrane for 1 week, mild inflammation response appeared without any other structural changes, indicating their good in vitro and in vivo biocompatibility. With injectability, uniformity, degradability, electroactivity, relative robustness, and biocompatibility, these eGels may have a huge potential as scaffolds for tissue regeneration and matrix for stimuli responsive drug release. Keywords: injectability, electroactivity, nanocomposite hydrogels, reinforcement, tetraaniline, alginate, gelatinWang QMWang QTeng WDove Medical PressarticleInjectabilityElectroactivityNanocomposite hydrogelsReinforcementTetraanilineAlginateGelatinMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 131-145 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Injectability Electroactivity Nanocomposite hydrogels Reinforcement Tetraaniline Alginate Gelatin Medicine (General) R5-920
spellingShingle	Injectability Electroactivity Nanocomposite hydrogels Reinforcement Tetraaniline Alginate Gelatin Medicine (General) R5-920 Wang QM Wang Q Teng W Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications
description	Qinmei Wang,1 Qiong Wang,2 Wei Teng2 1Laboratory of Biomaterials, Key Laboratory on Assisted Circulation, Ministry of Health, Cardiovascular Division, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Department of Prosthodontics, Hospital of Stomatology, Institute of Stomatological Research, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, People’s Republic of China Abstract: Injectable electroactive hydrogels (eGels) are promising in regenerative medicine and drug delivery, however, it is still a challenge to obtain such hydrogels simultaneously possessing other properties including uniform structure, degradability, robustness, and biocompatibility. An emerging strategy to endow hydrogels with desirable properties is to incorporate functional nanoparticles in their network. Herein, we report the synthesis and characterization of an injectable hydrogel based on oxidized alginate (OA) crosslinking gelatin reinforced by electroactive tetraaniline-graft-OA nanoparticles (nEOAs), where nEOAs are expected to impart electroactivity besides reinforcement without significantly degrading the other properties of hydrogels. Assays of transmission electron microscopy, 1H nuclear magnetic resonance, and dynamic light scattering reveal that EOA can spontaneously and quickly self-assemble into robust nanoparticles in water, and this nanoparticle structure can be kept at pH 3~9. Measurement of the gel time by rheometer and the stir bar method confirms the formation of the eGels, and their gel time is dependent on the weight content of nEOAs. As expected, adding nEOAs to hydrogels does not cause the phase separation (scanning electron microscopy observation), but it improves mechanical strength up to ~8 kPa and conductivity up to ~10-6 S/cm in our studied range. Incubating eGels in phosphate-buffered saline leads to their further swelling with an increase of water content <6% and gradual degradation. When growing mesenchymal stem cells on eGels with nEOA content ≤14%, the growth curves and morphology of cells were found to be similar to that on tissue culture plastic; when implanting these eGels on a chick chorioallantoic membrane for 1 week, mild inflammation response appeared without any other structural changes, indicating their good in vitro and in vivo biocompatibility. With injectability, uniformity, degradability, electroactivity, relative robustness, and biocompatibility, these eGels may have a huge potential as scaffolds for tissue regeneration and matrix for stimuli responsive drug release. Keywords: injectability, electroactivity, nanocomposite hydrogels, reinforcement, tetraaniline, alginate, gelatin
format	article
author	Wang QM Wang Q Teng W
author_facet	Wang QM Wang Q Teng W
author_sort	Wang QM
title	Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications
title_short	Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications
title_full	Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications
title_fullStr	Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications
title_full_unstemmed	Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications
title_sort	injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/a2f6021d71e94ddfa678a3c8da47ebe7
work_keys_str_mv	AT wangqm injectabledegradableelectroactivenanocompositehydrogelscontainingconductivepolymernanoparticlesforbiomedicalapplications AT wangq injectabledegradableelectroactivenanocompositehydrogelscontainingconductivepolymernanoparticlesforbiomedicalapplications AT tengw injectabledegradableelectroactivenanocompositehydrogelscontainingconductivepolymernanoparticlesforbiomedicalapplications
_version_	1718402240993034240

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

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