Environmental factors affecting the expression of pilAB as well as the proteome and transcriptome of the grass endophyte Azoarcus sp. strain BH72.
<h4>Background</h4>Bacterial communication is involved in regulation of cellular mechanisms such as metabolic processes, microbe-host interactions or biofilm formation. In the nitrogen-fixing model endophyte of grasses Azoarcus sp. strain BH72, known cell-cell signaling systems have not...
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oai:doaj.org-article:a2fd387757334b7ba27bd9f871805b572021-11-18T07:29:42ZEnvironmental factors affecting the expression of pilAB as well as the proteome and transcriptome of the grass endophyte Azoarcus sp. strain BH72.1932-620310.1371/journal.pone.0030421https://doaj.org/article/a2fd387757334b7ba27bd9f871805b572012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22276194/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Bacterial communication is involved in regulation of cellular mechanisms such as metabolic processes, microbe-host interactions or biofilm formation. In the nitrogen-fixing model endophyte of grasses Azoarcus sp. strain BH72, known cell-cell signaling systems have not been identified; however, the pilA gene encoding the structural protein of type IV pili that are essential for plant colonization appears to be regulated in a population density-dependent manner.<h4>Methodology/principal findings</h4>Our data suggest that pilAB expression is affected by population density, independent of autoinducers typical for gram-negative bacteria, likely depending on unknown secreted molecule(s) that can be produced by different bacterial species. We used transcriptomic and proteomic approaches to identify target genes and proteins differentially regulated in conditioned supernatants in comparison to standard growth conditions. Around 8% of the 3992 protein-coding genes of Azoarcus sp. and 18% of the detected proteins were differentially regulated. Regulatory proteins and transcription factors among the regulated proteins indicated a complex hierarchy. Differentially regulated genes and proteins were involved in processes such as type IV pili formation and regulation, metal and nutrient transport, energy metabolism, and unknown functions mediated by hypothetical proteins. Four of the newly discovered target genes were further analyzed and in general they showed regulation patterns similar to pilAB. The expression of one of them was shown to be induced in plant roots.<h4>Conclusion/significance</h4>This study is the first global approach to initiate characterization of cell density-dependent gene regulation mediated by soluble molecule(s) in the model endophyte Azoarcus sp. strain BH72. Our data suggest that the putative signaling molecule(s) are also produced by other Proteobacteria and might thus be used for interspecies communication. This study provides the foundation for the development of robust reporter systems for Azoarcus sp. to analyze mechanisms and molecules involved in the population-dependent gene expression in this endophyte in future.Lena Hauberg-LotteHannah KlingenbergChristian ScharfMelanie BöhmJörg PlesslFrauke FriedrichUwe VölkerAnke BeckerBarbara Reinhold-HurekPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30421 (2012) |
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Medicine R Science Q Lena Hauberg-Lotte Hannah Klingenberg Christian Scharf Melanie Böhm Jörg Plessl Frauke Friedrich Uwe Völker Anke Becker Barbara Reinhold-Hurek Environmental factors affecting the expression of pilAB as well as the proteome and transcriptome of the grass endophyte Azoarcus sp. strain BH72. |
| description |
<h4>Background</h4>Bacterial communication is involved in regulation of cellular mechanisms such as metabolic processes, microbe-host interactions or biofilm formation. In the nitrogen-fixing model endophyte of grasses Azoarcus sp. strain BH72, known cell-cell signaling systems have not been identified; however, the pilA gene encoding the structural protein of type IV pili that are essential for plant colonization appears to be regulated in a population density-dependent manner.<h4>Methodology/principal findings</h4>Our data suggest that pilAB expression is affected by population density, independent of autoinducers typical for gram-negative bacteria, likely depending on unknown secreted molecule(s) that can be produced by different bacterial species. We used transcriptomic and proteomic approaches to identify target genes and proteins differentially regulated in conditioned supernatants in comparison to standard growth conditions. Around 8% of the 3992 protein-coding genes of Azoarcus sp. and 18% of the detected proteins were differentially regulated. Regulatory proteins and transcription factors among the regulated proteins indicated a complex hierarchy. Differentially regulated genes and proteins were involved in processes such as type IV pili formation and regulation, metal and nutrient transport, energy metabolism, and unknown functions mediated by hypothetical proteins. Four of the newly discovered target genes were further analyzed and in general they showed regulation patterns similar to pilAB. The expression of one of them was shown to be induced in plant roots.<h4>Conclusion/significance</h4>This study is the first global approach to initiate characterization of cell density-dependent gene regulation mediated by soluble molecule(s) in the model endophyte Azoarcus sp. strain BH72. Our data suggest that the putative signaling molecule(s) are also produced by other Proteobacteria and might thus be used for interspecies communication. This study provides the foundation for the development of robust reporter systems for Azoarcus sp. to analyze mechanisms and molecules involved in the population-dependent gene expression in this endophyte in future. |
| format |
article |
| author |
Lena Hauberg-Lotte Hannah Klingenberg Christian Scharf Melanie Böhm Jörg Plessl Frauke Friedrich Uwe Völker Anke Becker Barbara Reinhold-Hurek |
| author_facet |
Lena Hauberg-Lotte Hannah Klingenberg Christian Scharf Melanie Böhm Jörg Plessl Frauke Friedrich Uwe Völker Anke Becker Barbara Reinhold-Hurek |
| author_sort |
Lena Hauberg-Lotte |
| title |
Environmental factors affecting the expression of pilAB as well as the proteome and transcriptome of the grass endophyte Azoarcus sp. strain BH72. |
| title_short |
Environmental factors affecting the expression of pilAB as well as the proteome and transcriptome of the grass endophyte Azoarcus sp. strain BH72. |
| title_full |
Environmental factors affecting the expression of pilAB as well as the proteome and transcriptome of the grass endophyte Azoarcus sp. strain BH72. |
| title_fullStr |
Environmental factors affecting the expression of pilAB as well as the proteome and transcriptome of the grass endophyte Azoarcus sp. strain BH72. |
| title_full_unstemmed |
Environmental factors affecting the expression of pilAB as well as the proteome and transcriptome of the grass endophyte Azoarcus sp. strain BH72. |
| title_sort |
environmental factors affecting the expression of pilab as well as the proteome and transcriptome of the grass endophyte azoarcus sp. strain bh72. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/a2fd387757334b7ba27bd9f871805b57 |
| work_keys_str_mv |
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