Synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery

Qing Fa Guo, Ting Ting Liu, Xi Yan, Xiu Hong Wang, Shuai Shi, Feng Luo, Zhi Yong QianState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People&#39;s Republic of ChinaBackground: Gene therapy is a promising approa...

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Autores principales:	Guo QF, Liu TT, Yan X, Wang XH, Shi S, Luo F, Qian ZY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a302515bc0174cb2a55f1925b32e6a0a
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spelling	oai:doaj.org-article:a302515bc0174cb2a55f1925b32e6a0a2021-12-02T02:31:46ZSynthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery1176-91141178-2013https://doaj.org/article/a302515bc0174cb2a55f1925b32e6a0a2011-08-01T00:00:00Zhttp://www.dovepress.com/synthesis-and-properties-of-a-novel-biodegradable-polyester-amine-copo-a8065https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Qing Fa Guo, Ting Ting Liu, Xi Yan, Xiu Hong Wang, Shuai Shi, Feng Luo, Zhi Yong QianState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People&#39;s Republic of ChinaBackground: Gene therapy is a promising approach to the treatment of a wide range of diseases. The development of efficient and adequate gene delivery systems could be one of the most important factors. Polyethyleneimine, a cationic polymer, is one of the most successful and widely used vectors for nonviral transfection in vitro and in vivo.Methods: A novel biodegradable poly(ester amine) copolymer (PEA) was successfully prepared from low molecular weight polyethylenimine (PEI, 2000 Da) and poly(L-lactide) copolymers.Results: According to the results of agarose gel electrophoresis, particle size and zeta potential measurement, and transfection efficiency, the PEA copolymers showed a good ability to condense plasmid DNA effectively into nanocomplexes with a small particle size (&le;150 nm) and moderate zeta potential (&ge;10 mV) at an appropriate polymeric carrier/DNA weight ratio. Compared with high molecular weight PEI (25kDa), the PEA obtained showed relatively high gene transfection efficiency as well as low cytotoxicity in vitro.Conclusion: These results indicate that such PEA might have potential application as a gene delivery system.Keywords: polyethylenimine, poly(L-lactide), gene delivery, cytotoxicity, transfection efficiencyGuo QFLiu TTYan XWang XHShi SLuo FQian ZYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1641-1649 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Guo QF Liu TT Yan X Wang XH Shi S Luo F Qian ZY Synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery
description	Qing Fa Guo, Ting Ting Liu, Xi Yan, Xiu Hong Wang, Shuai Shi, Feng Luo, Zhi Yong QianState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People&#39;s Republic of ChinaBackground: Gene therapy is a promising approach to the treatment of a wide range of diseases. The development of efficient and adequate gene delivery systems could be one of the most important factors. Polyethyleneimine, a cationic polymer, is one of the most successful and widely used vectors for nonviral transfection in vitro and in vivo.Methods: A novel biodegradable poly(ester amine) copolymer (PEA) was successfully prepared from low molecular weight polyethylenimine (PEI, 2000 Da) and poly(L-lactide) copolymers.Results: According to the results of agarose gel electrophoresis, particle size and zeta potential measurement, and transfection efficiency, the PEA copolymers showed a good ability to condense plasmid DNA effectively into nanocomplexes with a small particle size (&le;150 nm) and moderate zeta potential (&ge;10 mV) at an appropriate polymeric carrier/DNA weight ratio. Compared with high molecular weight PEI (25kDa), the PEA obtained showed relatively high gene transfection efficiency as well as low cytotoxicity in vitro.Conclusion: These results indicate that such PEA might have potential application as a gene delivery system.Keywords: polyethylenimine, poly(L-lactide), gene delivery, cytotoxicity, transfection efficiency
format	article
author	Guo QF Liu TT Yan X Wang XH Shi S Luo F Qian ZY
author_facet	Guo QF Liu TT Yan X Wang XH Shi S Luo F Qian ZY
author_sort	Guo QF
title	Synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery
title_short	Synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery
title_full	Synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery
title_fullStr	Synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery
title_full_unstemmed	Synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery
title_sort	synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(l-lactide) and low molecular weight polyethylenimine for gene delivery
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/a302515bc0174cb2a55f1925b32e6a0a
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Synthesis and properties of a novel biodegradable poly(ester amine) copolymer based on poly(L-lactide) and low molecular weight polyethylenimine for gene delivery

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