The <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage

Circadian clocks integrate environmental cues with endogenous signals to coordinate physiological outputs. Clock genes in plants are involved in many physiological and developmental processes, such as photosynthesis, stomata opening, stem elongation, light signaling, and floral induction. Many <i...

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Autores principales:	Nan Sun Kim, Su Jeong Kim, Jung Su Jo, Jun Gu Lee, Soo In Lee, Dong Hwan Kim, Jin A. Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Chinese cabbage circadian clock glucosinolate metabolite RNAi Genetics QH426-470
Acceso en línea:	https://doaj.org/article/a3128daf36954afcb806c60491e274e6
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spelling	oai:doaj.org-article:a3128daf36954afcb806c60491e274e62021-11-25T17:40:26ZThe <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage10.3390/genes121116642073-4425https://doaj.org/article/a3128daf36954afcb806c60491e274e62021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1664https://doaj.org/toc/2073-4425Circadian clocks integrate environmental cues with endogenous signals to coordinate physiological outputs. Clock genes in plants are involved in many physiological and developmental processes, such as photosynthesis, stomata opening, stem elongation, light signaling, and floral induction. Many <i>Brassicaceae</i> family plants, including Chinese cabbage (<i>Brassica rapa</i> ssp. <i>pekinensis</i>), produce a unique glucosinolate (GSL) secondary metabolite, which enhances plant protection, facilitates the design of functional foods, and has potential medical applications (e.g., as antidiabetic and anticancer agents). The levels of GSLs change diurnally, suggesting a connection to the circadian clock system. We investigated whether circadian clock genes affect the biosynthesis of GSLs in <i>Brassica rapa</i> using RNAi-mediated suppressed transgenic <i>Brassica rapa</i> <i>GIGENTEA</i> homolog (<i>BrGI</i> knockdown; hereafter GK1) Chinese cabbage. <i>GIGANTEA</i> plays an important role in the plant circadian clock system and is related to various developmental and metabolic processes. Using a validated GK1 transgenic line, we performed RNA sequencing and high-performance liquid chromatography analyses. The transcript levels of many GSL pathway genes were significantly altered in GK1 transgenic plants. In addition, GSL contents were substantially reduced in GK1 transgenic plants. We report that the <i>BrGI</i> circadian clock gene is required for the biosynthesis of GSLs in Chinese cabbage plants.Nan Sun KimSu Jeong KimJung Su JoJun Gu LeeSoo In LeeDong Hwan KimJin A. KimMDPI AGarticleChinese cabbagecircadian clockglucosinolatemetaboliteRNAiGeneticsQH426-470ENGenes, Vol 12, Iss 1664, p 1664 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chinese cabbage circadian clock glucosinolate metabolite RNAi Genetics QH426-470
spellingShingle	Chinese cabbage circadian clock glucosinolate metabolite RNAi Genetics QH426-470 Nan Sun Kim Su Jeong Kim Jung Su Jo Jun Gu Lee Soo In Lee Dong Hwan Kim Jin A. Kim The <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage
description	Circadian clocks integrate environmental cues with endogenous signals to coordinate physiological outputs. Clock genes in plants are involved in many physiological and developmental processes, such as photosynthesis, stomata opening, stem elongation, light signaling, and floral induction. Many <i>Brassicaceae</i> family plants, including Chinese cabbage (<i>Brassica rapa</i> ssp. <i>pekinensis</i>), produce a unique glucosinolate (GSL) secondary metabolite, which enhances plant protection, facilitates the design of functional foods, and has potential medical applications (e.g., as antidiabetic and anticancer agents). The levels of GSLs change diurnally, suggesting a connection to the circadian clock system. We investigated whether circadian clock genes affect the biosynthesis of GSLs in <i>Brassica rapa</i> using RNAi-mediated suppressed transgenic <i>Brassica rapa</i> <i>GIGENTEA</i> homolog (<i>BrGI</i> knockdown; hereafter GK1) Chinese cabbage. <i>GIGANTEA</i> plays an important role in the plant circadian clock system and is related to various developmental and metabolic processes. Using a validated GK1 transgenic line, we performed RNA sequencing and high-performance liquid chromatography analyses. The transcript levels of many GSL pathway genes were significantly altered in GK1 transgenic plants. In addition, GSL contents were substantially reduced in GK1 transgenic plants. We report that the <i>BrGI</i> circadian clock gene is required for the biosynthesis of GSLs in Chinese cabbage plants.
format	article
author	Nan Sun Kim Su Jeong Kim Jung Su Jo Jun Gu Lee Soo In Lee Dong Hwan Kim Jin A. Kim
author_facet	Nan Sun Kim Su Jeong Kim Jung Su Jo Jun Gu Lee Soo In Lee Dong Hwan Kim Jin A. Kim
author_sort	Nan Sun Kim
title	The <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage
title_short	The <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage
title_full	The <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage
title_fullStr	The <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage
title_full_unstemmed	The <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage
title_sort	<i>brgi</i> circadian clock gene is involved in the regulation of glucosinolates in chinese cabbage
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a3128daf36954afcb806c60491e274e6
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The <i>BrGI</i> Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage

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