Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile

Fecal microbiota transplantation has been shown to be an effective treatment for patients with recurrent C. difficile colitis. Although fecal microbiota transplantation helps to re-establish a normal gut function in patients, the extent of the repopulation of the recipient microbial community varies...

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Autores principales:	Ranjit Kumar, Nengjun Yi, Degui Zhi, Peter Eipers, Kelly T. Goldsmith, Paula Dixon, David K. Crossman, Michael R. Crowley, Elliot J. Lefkowitz, J. Martin Rodriguez, Casey D. Morrow
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/a3146234b9e64d2598f04b4b36d30d01
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spelling	oai:doaj.org-article:a3146234b9e64d2598f04b4b36d30d012021-12-02T11:42:16ZIdentification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile10.1038/s41522-017-0020-72055-5008https://doaj.org/article/a3146234b9e64d2598f04b4b36d30d012017-06-01T00:00:00Zhttps://doi.org/10.1038/s41522-017-0020-7https://doaj.org/toc/2055-5008Fecal microbiota transplantation has been shown to be an effective treatment for patients with recurrent C. difficile colitis. Although fecal microbiota transplantation helps to re-establish a normal gut function in patients, the extent of the repopulation of the recipient microbial community varies. To further understand this variation, it is important to determine the fate of donor microbes in the patients following fecal microbiota transplantation. We have developed a new method that utilizes the unique single nucleotide variants of gut microbes to accurately identify microbes in paired fecal samples from the same individual taken at different times. Using this method, we identified transplant donor microbes in seven recipients 3–6 months after fecal microbiota transplantation; in two of these fecal microbiota transplantation, we were able to identify donor microbes that persist in recipients up to 2 years post-fecal microbiota transplantation. Our study provides new insights into the dynamics of the reconstitution of the gastrointestinal microbe community structure following fecal microbiota transplantation.Ranjit KumarNengjun YiDegui ZhiPeter EipersKelly T. GoldsmithPaula DixonDavid K. CrossmanMichael R. CrowleyElliot J. LefkowitzJ. Martin RodriguezCasey D. MorrowNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 3, Iss 1, Pp 1-4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Ranjit Kumar Nengjun Yi Degui Zhi Peter Eipers Kelly T. Goldsmith Paula Dixon David K. Crossman Michael R. Crowley Elliot J. Lefkowitz J. Martin Rodriguez Casey D. Morrow Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile
description	Fecal microbiota transplantation has been shown to be an effective treatment for patients with recurrent C. difficile colitis. Although fecal microbiota transplantation helps to re-establish a normal gut function in patients, the extent of the repopulation of the recipient microbial community varies. To further understand this variation, it is important to determine the fate of donor microbes in the patients following fecal microbiota transplantation. We have developed a new method that utilizes the unique single nucleotide variants of gut microbes to accurately identify microbes in paired fecal samples from the same individual taken at different times. Using this method, we identified transplant donor microbes in seven recipients 3–6 months after fecal microbiota transplantation; in two of these fecal microbiota transplantation, we were able to identify donor microbes that persist in recipients up to 2 years post-fecal microbiota transplantation. Our study provides new insights into the dynamics of the reconstitution of the gastrointestinal microbe community structure following fecal microbiota transplantation.
format	article
author	Ranjit Kumar Nengjun Yi Degui Zhi Peter Eipers Kelly T. Goldsmith Paula Dixon David K. Crossman Michael R. Crowley Elliot J. Lefkowitz J. Martin Rodriguez Casey D. Morrow
author_facet	Ranjit Kumar Nengjun Yi Degui Zhi Peter Eipers Kelly T. Goldsmith Paula Dixon David K. Crossman Michael R. Crowley Elliot J. Lefkowitz J. Martin Rodriguez Casey D. Morrow
author_sort	Ranjit Kumar
title	Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile
title_short	Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile
title_full	Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile
title_fullStr	Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile
title_full_unstemmed	Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile
title_sort	identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent clostridium difficile
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a3146234b9e64d2598f04b4b36d30d01
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Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile

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