Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing

It is largely assumed that the teleost retina shows continuous and active proliferative and neurogenic activity throughout life. However, when delving into the teleost literature, one finds that assumptions about a highly active and continuous proliferation in the adult retina are based on studies i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ismael Hernández-Núñez, Ana Quelle-Regaldie, Laura Sánchez, Fátima Adrio, Eva Candal, Antón Barreiro-Iglesias
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	retina zebrafish proliferation secondary neurogenesis PCNA pH3 Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a314e30a0c484e4f9363d4a1d3e65a8a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a314e30a0c484e4f9363d4a1d3e65a8a
record_format	dspace
spelling	oai:doaj.org-article:a314e30a0c484e4f9363d4a1d3e65a8a2021-11-11T17:10:20ZDecline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing10.3390/ijms2221117151422-00671661-6596https://doaj.org/article/a314e30a0c484e4f9363d4a1d3e65a8a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11715https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067It is largely assumed that the teleost retina shows continuous and active proliferative and neurogenic activity throughout life. However, when delving into the teleost literature, one finds that assumptions about a highly active and continuous proliferation in the adult retina are based on studies in which proliferation was not quantified in a comparative way at the different life stages or was mainly studied in juveniles/young adults. Here, we performed a systematic and comparative study of the constitutive proliferative activity of the retina from early developing (2 days post-fertilisation) to aged (up to 3–4 years post-fertilisation) zebrafish. The mitotic activity and cell cycle progression were analysed by using immunofluorescence against pH3 and PCNA, respectively. We observed a decline in the cell proliferation in the retina with ageing despite the occurrence of a wave of secondary proliferation during sexual maturation. During this wave of secondary proliferation, the distribution of proliferating and mitotic cells changes from the inner to the outer nuclear layer in the central retina. Importantly, in aged zebrafish, there is a virtual disappearance of mitotic activity. Our results showing a decline in the proliferative activity of the zebrafish retina with ageing are of crucial importance since it is generally assumed that the fish retina has continuous proliferative activity throughout life.Ismael Hernández-NúñezAna Quelle-RegaldieLaura SánchezFátima AdrioEva CandalAntón Barreiro-IglesiasMDPI AGarticleretinazebrafishproliferationsecondary neurogenesisPCNApH3Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11715, p 11715 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	retina zebrafish proliferation secondary neurogenesis PCNA pH3 Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	retina zebrafish proliferation secondary neurogenesis PCNA pH3 Biology (General) QH301-705.5 Chemistry QD1-999 Ismael Hernández-Núñez Ana Quelle-Regaldie Laura Sánchez Fátima Adrio Eva Candal Antón Barreiro-Iglesias Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing
description	It is largely assumed that the teleost retina shows continuous and active proliferative and neurogenic activity throughout life. However, when delving into the teleost literature, one finds that assumptions about a highly active and continuous proliferation in the adult retina are based on studies in which proliferation was not quantified in a comparative way at the different life stages or was mainly studied in juveniles/young adults. Here, we performed a systematic and comparative study of the constitutive proliferative activity of the retina from early developing (2 days post-fertilisation) to aged (up to 3–4 years post-fertilisation) zebrafish. The mitotic activity and cell cycle progression were analysed by using immunofluorescence against pH3 and PCNA, respectively. We observed a decline in the cell proliferation in the retina with ageing despite the occurrence of a wave of secondary proliferation during sexual maturation. During this wave of secondary proliferation, the distribution of proliferating and mitotic cells changes from the inner to the outer nuclear layer in the central retina. Importantly, in aged zebrafish, there is a virtual disappearance of mitotic activity. Our results showing a decline in the proliferative activity of the zebrafish retina with ageing are of crucial importance since it is generally assumed that the fish retina has continuous proliferative activity throughout life.
format	article
author	Ismael Hernández-Núñez Ana Quelle-Regaldie Laura Sánchez Fátima Adrio Eva Candal Antón Barreiro-Iglesias
author_facet	Ismael Hernández-Núñez Ana Quelle-Regaldie Laura Sánchez Fátima Adrio Eva Candal Antón Barreiro-Iglesias
author_sort	Ismael Hernández-Núñez
title	Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing
title_short	Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing
title_full	Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing
title_fullStr	Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing
title_full_unstemmed	Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing
title_sort	decline in constitutive proliferative activity in the zebrafish retina with ageing
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a314e30a0c484e4f9363d4a1d3e65a8a
work_keys_str_mv	AT ismaelhernandeznunez declineinconstitutiveproliferativeactivityinthezebrafishretinawithageing AT anaquelleregaldie declineinconstitutiveproliferativeactivityinthezebrafishretinawithageing AT laurasanchez declineinconstitutiveproliferativeactivityinthezebrafishretinawithageing AT fatimaadrio declineinconstitutiveproliferativeactivityinthezebrafishretinawithageing AT evacandal declineinconstitutiveproliferativeactivityinthezebrafishretinawithageing AT antonbarreiroiglesias declineinconstitutiveproliferativeactivityinthezebrafishretinawithageing
_version_	1718432193894678528

Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing

Ejemplares similares