Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline

Abstract Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have been studied for their therapeutic potential. However, evaluating the quality of hBM-MSCs before transplantation remains a challenge. We addressed this issue in the present study by investigating deformation, the expression of...

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Autores principales:	Tae Hwan Shin, Seungah Lee, Ki Ryung Choi, Da Yeon Lee, Yongman Kim, Man Jeong Paik, Chan Seo, Seok Kang, Moon Suk Jin, Tae Hyeon Yoo, Seong Ho Kang, Gwang Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a3150020075748028c38a7708b449744
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spelling	oai:doaj.org-article:a3150020075748028c38a7708b4497442021-12-02T12:32:37ZQuality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline10.1038/s41598-017-01315-02045-2322https://doaj.org/article/a3150020075748028c38a7708b4497442017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01315-0https://doaj.org/toc/2045-2322Abstract Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have been studied for their therapeutic potential. However, evaluating the quality of hBM-MSCs before transplantation remains a challenge. We addressed this issue in the present study by investigating deformation, the expression of genes related to reactive oxygen species (ROS) generation, changes in amino acid profiles, and membrane fluidity in hBM-MSCs. Deformability and cell size were decreased after storage for 6 and 12 h, respectively, in phosphate-buffered saline. Intracellular ROS levels also increased over time, which was associated with altered expression of genes related to ROS generation and amino acid metabolism. Membrane fluidity measurements revealed higher Laurdan generalized polarization values at 6 and 12 h; however, this effect was reversed by N-acetyl-l-cysteine-treatment. These findings indicate that the quality and freshness of hBM-MSCs is lost over time after dissociation from the culture dish for transplantation, highlighting the importance of using freshly trypsinized cells in clinical applications.Tae Hwan ShinSeungah LeeKi Ryung ChoiDa Yeon LeeYongman KimMan Jeong PaikChan SeoSeok KangMoon Suk JinTae Hyeon YooSeong Ho KangGwang LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tae Hwan Shin Seungah Lee Ki Ryung Choi Da Yeon Lee Yongman Kim Man Jeong Paik Chan Seo Seok Kang Moon Suk Jin Tae Hyeon Yoo Seong Ho Kang Gwang Lee Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline
description	Abstract Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have been studied for their therapeutic potential. However, evaluating the quality of hBM-MSCs before transplantation remains a challenge. We addressed this issue in the present study by investigating deformation, the expression of genes related to reactive oxygen species (ROS) generation, changes in amino acid profiles, and membrane fluidity in hBM-MSCs. Deformability and cell size were decreased after storage for 6 and 12 h, respectively, in phosphate-buffered saline. Intracellular ROS levels also increased over time, which was associated with altered expression of genes related to ROS generation and amino acid metabolism. Membrane fluidity measurements revealed higher Laurdan generalized polarization values at 6 and 12 h; however, this effect was reversed by N-acetyl-l-cysteine-treatment. These findings indicate that the quality and freshness of hBM-MSCs is lost over time after dissociation from the culture dish for transplantation, highlighting the importance of using freshly trypsinized cells in clinical applications.
format	article
author	Tae Hwan Shin Seungah Lee Ki Ryung Choi Da Yeon Lee Yongman Kim Man Jeong Paik Chan Seo Seok Kang Moon Suk Jin Tae Hyeon Yoo Seong Ho Kang Gwang Lee
author_facet	Tae Hwan Shin Seungah Lee Ki Ryung Choi Da Yeon Lee Yongman Kim Man Jeong Paik Chan Seo Seok Kang Moon Suk Jin Tae Hyeon Yoo Seong Ho Kang Gwang Lee
author_sort	Tae Hwan Shin
title	Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline
title_short	Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline
title_full	Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline
title_fullStr	Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline
title_full_unstemmed	Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline
title_sort	quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a3150020075748028c38a7708b449744
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_version_	1718394052391469056

Quality and freshness of human bone marrow-derived mesenchymal stem cells decrease over time after trypsinization and storage in phosphate-buffered saline

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