Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods

Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods

Francesco Conversano,1 Giulia Soloperto,1 Antonio Greco,1 Andrea Ragusa,1,2 Ernesto Casciaro,1 Fernanda Chiriacò,1 Christian Demitri,3 Giuseppe Gigli,2–5 Alfonso Maffezzoli,3 Sergio Casciaro11National Research Council, Institute of Clinical Physiology, Lecce, Italy; 2Nat...

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Autores principales: Conversano F, Soloperto G, Greco A, Ragusa A, Casciaro E, Chiriacò F, Demitri C, Gigli G, Maffezzoli A, Casciaro S
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/a318e01a148a4f65bcf2a4146d189a2f
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spelling oai:doaj.org-article:a318e01a148a4f65bcf2a4146d189a2f2021-12-02T05:02:11ZEchographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods1176-91141178-2013https://doaj.org/article/a318e01a148a4f65bcf2a4146d189a2f2012-08-01T00:00:00Zhttp://www.dovepress.com/echographic-detectability-of-optoacoustic-signals-from-low-concentrati-a10653https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Francesco Conversano,1 Giulia Soloperto,1 Antonio Greco,1 Andrea Ragusa,1,2 Ernesto Casciaro,1 Fernanda Chiriacò,1 Christian Demitri,3 Giuseppe Gigli,2–5 Alfonso Maffezzoli,3 Sergio Casciaro11National Research Council, Institute of Clinical Physiology, Lecce, Italy; 2National Nanotechnology Laboratory of CNR-NANO, Lecce, Italy; 3University of Salento, Department of Engineering for Innovation, Lecce, Italy; 4Italian Institute of Technology – Center for Biomolecular Nanotechnology (CBN-IIT), Arnesano, Italy; 5University of Salento, Department of Mathematics and Physics ‘Ennio De Giorgi’, Lecce, ItalyPurpose: To evaluate the diagnostic performance of gold nanorod (GNR)-enhanced optoacoustic imaging employing a conventional echographic device and to determine the most effective operative configuration in order to assure optoacoustic effectiveness, nanoparticle stability, and imaging procedure safety.Methods: The most suitable laser parameters were experimentally determined in order to assure nanoparticle stability during the optoacoustic imaging procedures. The selected configuration was then applied to a novel tissue-mimicking phantom, in which GNR solutions covering a wide range of low concentrations (25–200 pM) and different sample volumes (50–200 µL) were exposed to pulsed laser irradiation. GNR-emitted optoacoustic signals were acquired either by a couple of single-element ultrasound probes or by an echographic transducer. Off-line analysis included: (a) quantitative evaluation of the relationships between GNR concentration, sample volume, phantom geometry, and amplitude of optoacoustic signals propagating along different directions; (b) echographic detection of “optoacoustic spots,” analyzing their intensity, spatial distribution, and clinical exploitability. MTT measurements performed on two different cell lines were also used to quantify biocompatibility of the synthesized GNRs in the adopted doses.Results: Laser irradiation at 30 mJ/cm2 for 20 seconds resulted in the best compromise among the requirements of effectiveness, safety, and nanoparticle stability. Amplitude of GNR-emitted optoacoustic pulses was proportional to both sample volume and concentration along each considered propagation direction for all the tested boundary conditions, providing an experimental confirmation of isotropic optoacoustic emission. Average intensity of echographically detected spots showed similar behavior, emphasizing the presence of an “ideal” GNR concentration (100 pM) that optimized optoacoustic effectiveness. The tested GNRs also exhibited high biocompatibility over the entire considered concentration range.Conclusion: An optimal configuration for GNR-enhanced optoacoustic imaging was experimentally determined, demonstrating in particular its feasibility with a conventional echographic device. The proposed approach can be easily extended to quantitative performance evaluation of different contrast agents for optoacoustic imaging.Keywords: photoacoustic imaging, tissue-mimicking phantom, laser, nanoparticle degradationConversano FSoloperto GGreco ARagusa ACasciaro EChiriacò FDemitri CGigli GMaffezzoli ACasciaro SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4373-4389 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Conversano F
Soloperto G
Greco A
Ragusa A
Casciaro E
Chiriacò F
Demitri C
Gigli G
Maffezzoli A
Casciaro S
Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods
description Francesco Conversano,1 Giulia Soloperto,1 Antonio Greco,1 Andrea Ragusa,1,2 Ernesto Casciaro,1 Fernanda Chiriacò,1 Christian Demitri,3 Giuseppe Gigli,2–5 Alfonso Maffezzoli,3 Sergio Casciaro11National Research Council, Institute of Clinical Physiology, Lecce, Italy; 2National Nanotechnology Laboratory of CNR-NANO, Lecce, Italy; 3University of Salento, Department of Engineering for Innovation, Lecce, Italy; 4Italian Institute of Technology – Center for Biomolecular Nanotechnology (CBN-IIT), Arnesano, Italy; 5University of Salento, Department of Mathematics and Physics ‘Ennio De Giorgi’, Lecce, ItalyPurpose: To evaluate the diagnostic performance of gold nanorod (GNR)-enhanced optoacoustic imaging employing a conventional echographic device and to determine the most effective operative configuration in order to assure optoacoustic effectiveness, nanoparticle stability, and imaging procedure safety.Methods: The most suitable laser parameters were experimentally determined in order to assure nanoparticle stability during the optoacoustic imaging procedures. The selected configuration was then applied to a novel tissue-mimicking phantom, in which GNR solutions covering a wide range of low concentrations (25–200 pM) and different sample volumes (50–200 µL) were exposed to pulsed laser irradiation. GNR-emitted optoacoustic signals were acquired either by a couple of single-element ultrasound probes or by an echographic transducer. Off-line analysis included: (a) quantitative evaluation of the relationships between GNR concentration, sample volume, phantom geometry, and amplitude of optoacoustic signals propagating along different directions; (b) echographic detection of “optoacoustic spots,” analyzing their intensity, spatial distribution, and clinical exploitability. MTT measurements performed on two different cell lines were also used to quantify biocompatibility of the synthesized GNRs in the adopted doses.Results: Laser irradiation at 30 mJ/cm2 for 20 seconds resulted in the best compromise among the requirements of effectiveness, safety, and nanoparticle stability. Amplitude of GNR-emitted optoacoustic pulses was proportional to both sample volume and concentration along each considered propagation direction for all the tested boundary conditions, providing an experimental confirmation of isotropic optoacoustic emission. Average intensity of echographically detected spots showed similar behavior, emphasizing the presence of an “ideal” GNR concentration (100 pM) that optimized optoacoustic effectiveness. The tested GNRs also exhibited high biocompatibility over the entire considered concentration range.Conclusion: An optimal configuration for GNR-enhanced optoacoustic imaging was experimentally determined, demonstrating in particular its feasibility with a conventional echographic device. The proposed approach can be easily extended to quantitative performance evaluation of different contrast agents for optoacoustic imaging.Keywords: photoacoustic imaging, tissue-mimicking phantom, laser, nanoparticle degradation
format article
author Conversano F
Soloperto G
Greco A
Ragusa A
Casciaro E
Chiriacò F
Demitri C
Gigli G
Maffezzoli A
Casciaro S
author_facet Conversano F
Soloperto G
Greco A
Ragusa A
Casciaro E
Chiriacò F
Demitri C
Gigli G
Maffezzoli A
Casciaro S
author_sort Conversano F
title Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods
title_short Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods
title_full Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods
title_fullStr Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods
title_full_unstemmed Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods
title_sort echographic detectability of optoacoustic signals from low-concentration peg-coated gold nanorods
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/a318e01a148a4f65bcf2a4146d189a2f
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