Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications

Natural extracts have been of very high interest since ancient time due to their enormous medicinal use and researcher’s attention have further gone up recently to explore their phytochemical compositions, properties, potential applications in the areas such as, cosmetics, foods etc. In this present...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nitesh Bhalla, Nitin Ingle, Srilakshmi V. Patri, D. Haranath
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Moringa Gas chromatography-mass spectra Phytochemicals Free radical scavenging IC50 Antioxidant Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a31e4c99a3f04118875c4513b4be1ebe
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a31e4c99a3f04118875c4513b4be1ebe
record_format	dspace
spelling	oai:doaj.org-article:a31e4c99a3f04118875c4513b4be1ebe2021-11-20T04:56:34ZPhytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications1319-562X10.1016/j.sjbs.2021.07.075https://doaj.org/article/a31e4c99a3f04118875c4513b4be1ebe2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006586https://doaj.org/toc/1319-562XNatural extracts have been of very high interest since ancient time due to their enormous medicinal use and researcher’s attention have further gone up recently to explore their phytochemical compositions, properties, potential applications in the areas such as, cosmetics, foods etc. In this present study phytochemical analysis have been done on the aqueous and methanolic Moringa leaves extracts using Gas Chromatography-Mass spectrometry (GCMS) and their free radical scavenging potency (FRSP) studied using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical for further applications. GCMS analysis revealed an extraction of range of phytochemicals in aqueous and methanolic extracts. In aqueous, extract constituents found with high percent peak area are Carbonic acid, butyl 2-pentyl ester (20.64%), 2-Isopropoxyethyl propionate (16.87%), Butanedioic acid, 2-hydroxy-2-methyl-, (3.14%) (also known as Citramalic acid that has been rarely detected in plant extracts) and many other phytochemicals were detected. Similarly, fifty-four bio components detected in methanolic extract of Moringa leaves, which were relatively higher than the aqueous extract. Few major compounds found with high percent peak area are 1,3-Propanediol, 2-ethyl-2- (hydroxymethyl)- (21.19%), Propionic acid, 2-methyl-, octyl ester (15.02%), Ethanamine, N-ethyl-N-nitroso- (5.21%), and 9,12,15-Octadecatrienoic acid etc. FRSP for methanolic extract was also recorded much higher than aqueous extract. The half-maximal inhibitory concentration (IC50) of Moringa aqueous extract observed is 4.65 µl/ml and for methanolic extract 1.83 µl/ml. These extracts can act as very powerful antioxidants, anti-inflammatory ingredient for various applications in diverse field of food, cosmetics, medicine etc.Nitesh BhallaNitin IngleSrilakshmi V. PatriD. HaranathElsevierarticleMoringaGas chromatography-mass spectraPhytochemicalsFree radical scavengingIC50AntioxidantBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6915-6928 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Moringa Gas chromatography-mass spectra Phytochemicals Free radical scavenging IC50 Antioxidant Biology (General) QH301-705.5
spellingShingle	Moringa Gas chromatography-mass spectra Phytochemicals Free radical scavenging IC50 Antioxidant Biology (General) QH301-705.5 Nitesh Bhalla Nitin Ingle Srilakshmi V. Patri D. Haranath Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications
description	Natural extracts have been of very high interest since ancient time due to their enormous medicinal use and researcher’s attention have further gone up recently to explore their phytochemical compositions, properties, potential applications in the areas such as, cosmetics, foods etc. In this present study phytochemical analysis have been done on the aqueous and methanolic Moringa leaves extracts using Gas Chromatography-Mass spectrometry (GCMS) and their free radical scavenging potency (FRSP) studied using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical for further applications. GCMS analysis revealed an extraction of range of phytochemicals in aqueous and methanolic extracts. In aqueous, extract constituents found with high percent peak area are Carbonic acid, butyl 2-pentyl ester (20.64%), 2-Isopropoxyethyl propionate (16.87%), Butanedioic acid, 2-hydroxy-2-methyl-, (3.14%) (also known as Citramalic acid that has been rarely detected in plant extracts) and many other phytochemicals were detected. Similarly, fifty-four bio components detected in methanolic extract of Moringa leaves, which were relatively higher than the aqueous extract. Few major compounds found with high percent peak area are 1,3-Propanediol, 2-ethyl-2- (hydroxymethyl)- (21.19%), Propionic acid, 2-methyl-, octyl ester (15.02%), Ethanamine, N-ethyl-N-nitroso- (5.21%), and 9,12,15-Octadecatrienoic acid etc. FRSP for methanolic extract was also recorded much higher than aqueous extract. The half-maximal inhibitory concentration (IC50) of Moringa aqueous extract observed is 4.65 µl/ml and for methanolic extract 1.83 µl/ml. These extracts can act as very powerful antioxidants, anti-inflammatory ingredient for various applications in diverse field of food, cosmetics, medicine etc.
format	article
author	Nitesh Bhalla Nitin Ingle Srilakshmi V. Patri D. Haranath
author_facet	Nitesh Bhalla Nitin Ingle Srilakshmi V. Patri D. Haranath
author_sort	Nitesh Bhalla
title	Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications
title_short	Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications
title_full	Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications
title_fullStr	Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications
title_full_unstemmed	Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications
title_sort	phytochemical analysis of moringa oleifera leaves extracts by gc-ms and free radical scavenging potency for industrial applications
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a31e4c99a3f04118875c4513b4be1ebe
work_keys_str_mv	AT niteshbhalla phytochemicalanalysisofmoringaoleiferaleavesextractsbygcmsandfreeradicalscavengingpotencyforindustrialapplications AT nitiningle phytochemicalanalysisofmoringaoleiferaleavesextractsbygcmsandfreeradicalscavengingpotencyforindustrialapplications AT srilakshmivpatri phytochemicalanalysisofmoringaoleiferaleavesextractsbygcmsandfreeradicalscavengingpotencyforindustrialapplications AT dharanath phytochemicalanalysisofmoringaoleiferaleavesextractsbygcmsandfreeradicalscavengingpotencyforindustrialapplications
_version_	1718419731649658880

Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications

Ejemplares similares