Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy
Li Gao,1 Qin Li,1 Zebin Deng,1 Brendan Brady,2 Ni Xia,1 Yang Zhou,1 Haixia Shi3 1Institute of Life Sciences, Jiangsu University, Zhenjiang, 2Department of Physics, University of Victoria, Victoria, BC, Canada, 3Department of Physical Education, Dalian Jiaotong University, Dalian, People’s...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2017
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/a322089f8bb14671b099b3629f98123d |
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| Sumario: | Li Gao,1 Qin Li,1 Zebin Deng,1 Brendan Brady,2 Ni Xia,1 Yang Zhou,1 Haixia Shi3 1Institute of Life Sciences, Jiangsu University, Zhenjiang, 2Department of Physics, University of Victoria, Victoria, BC, Canada, 3Department of Physical Education, Dalian Jiaotong University, Dalian, People’s Republic of China Abstract: Molybdenum disulfide (MoS2) has shown highly attractive superiority as a platform for sensing. However, DNA physisorption on the surface of MoS2 was susceptible to nonspecific probe displacement and false-positive signals. To solve these problems, we have developed a novel MoS2–aptamer nanosheet biosensor for detecting thrombin using a covalently linked aptamer to the MoS2 nanosheet. Ten percent Tween 80 was used to prevent thrombin from nonspecific binding and to rapidly form thiol-DNA/gold nanoparticle (AuNP) conjugates. Furthermore, an MoS2 and exonuclease coassisted signal amplification strategy was developed to improve the detection limit for thrombin. We used the hybridization of the aptamer molecules and the matched strand with a 5' terminal thiol to immobilize the aptamer molecules on the surface of AuNPs in AuNPs@MoS2 nanocomposites. Exonuclease digested the single-strand aptamer and released the thrombin, which was then detected in the next recycle. With the coassisted amplification strategy, a 6 fM detection limit was achieved, showing that this method has higher sensitivity than most reported methods for thrombin detection. The results presented in this work show that this method of covalently attaching the aptamer and using the coassisted amplification is a promising technique for the detection of protein in medical diagnostics. Keywords: Molybdenum disulfide, aptamer, thrombin, protein detection, high sensitivity |
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