Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy

Li Gao,1 Qin Li,1 Zebin Deng,1 Brendan Brady,2 Ni Xia,1 Yang Zhou,1 Haixia Shi3 1Institute of Life Sciences, Jiangsu University, Zhenjiang, 2Department of Physics, University of Victoria, Victoria, BC, Canada, 3Department of Physical Education, Dalian Jiaotong University, Dalian, People’s...

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Autores principales:	Gao L, Li Q, Deng Z, Brady B, Xia N, Zhou Y, Shi H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Molybdenum disulfide (MoS2) Aptamer Thrombin Protein Detection High sensitivity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a322089f8bb14671b099b3629f98123d
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spelling	oai:doaj.org-article:a322089f8bb14671b099b3629f98123d2021-12-02T06:37:58ZHighly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy1178-2013https://doaj.org/article/a322089f8bb14671b099b3629f98123d2017-10-01T00:00:00Zhttps://www.dovepress.com/highly-sensitive-protein-detection-via-covalently-linked-aptamer-to-mo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Li Gao,1 Qin Li,1 Zebin Deng,1 Brendan Brady,2 Ni Xia,1 Yang Zhou,1 Haixia Shi3 1Institute of Life Sciences, Jiangsu University, Zhenjiang, 2Department of Physics, University of Victoria, Victoria, BC, Canada, 3Department of Physical Education, Dalian Jiaotong University, Dalian, People’s Republic of China Abstract: Molybdenum disulfide (MoS2) has shown highly attractive superiority as a platform for sensing. However, DNA physisorption on the surface of MoS2 was susceptible to nonspecific probe displacement and false-positive signals. To solve these problems, we have developed a novel MoS2–aptamer nanosheet biosensor for detecting thrombin using a covalently linked aptamer to the MoS2 nanosheet. Ten percent Tween 80 was used to prevent thrombin from nonspecific binding and to rapidly form thiol-DNA/gold nanoparticle (AuNP) conjugates. Furthermore, an MoS2 and exonuclease coassisted signal amplification strategy was developed to improve the detection limit for thrombin. We used the hybridization of the aptamer molecules and the matched strand with a 5' terminal thiol to immobilize the aptamer molecules on the surface of AuNPs in AuNPs@MoS2 nanocomposites. Exonuclease digested the single-strand aptamer and released the thrombin, which was then detected in the next recycle. With the coassisted amplification strategy, a 6 fM detection limit was achieved, showing that this method has higher sensitivity than most reported methods for thrombin detection. The results presented in this work show that this method of covalently attaching the aptamer and using the coassisted amplification is a promising technique for the detection of protein in medical diagnostics. Keywords: Molybdenum disulfide, aptamer, thrombin, protein detection, high sensitivityGao LLi QDeng ZBrady BXia NZhou YShi HDove Medical PressarticleMolybdenum disulfide (MoS2)AptamerThrombinProtein DetectionHigh sensitivityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7847-7853 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Molybdenum disulfide (MoS2) Aptamer Thrombin Protein Detection High sensitivity Medicine (General) R5-920
spellingShingle	Molybdenum disulfide (MoS2) Aptamer Thrombin Protein Detection High sensitivity Medicine (General) R5-920 Gao L Li Q Deng Z Brady B Xia N Zhou Y Shi H Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy
description	Li Gao,1 Qin Li,1 Zebin Deng,1 Brendan Brady,2 Ni Xia,1 Yang Zhou,1 Haixia Shi3 1Institute of Life Sciences, Jiangsu University, Zhenjiang, 2Department of Physics, University of Victoria, Victoria, BC, Canada, 3Department of Physical Education, Dalian Jiaotong University, Dalian, People’s Republic of China Abstract: Molybdenum disulfide (MoS2) has shown highly attractive superiority as a platform for sensing. However, DNA physisorption on the surface of MoS2 was susceptible to nonspecific probe displacement and false-positive signals. To solve these problems, we have developed a novel MoS2–aptamer nanosheet biosensor for detecting thrombin using a covalently linked aptamer to the MoS2 nanosheet. Ten percent Tween 80 was used to prevent thrombin from nonspecific binding and to rapidly form thiol-DNA/gold nanoparticle (AuNP) conjugates. Furthermore, an MoS2 and exonuclease coassisted signal amplification strategy was developed to improve the detection limit for thrombin. We used the hybridization of the aptamer molecules and the matched strand with a 5' terminal thiol to immobilize the aptamer molecules on the surface of AuNPs in AuNPs@MoS2 nanocomposites. Exonuclease digested the single-strand aptamer and released the thrombin, which was then detected in the next recycle. With the coassisted amplification strategy, a 6 fM detection limit was achieved, showing that this method has higher sensitivity than most reported methods for thrombin detection. The results presented in this work show that this method of covalently attaching the aptamer and using the coassisted amplification is a promising technique for the detection of protein in medical diagnostics. Keywords: Molybdenum disulfide, aptamer, thrombin, protein detection, high sensitivity
format	article
author	Gao L Li Q Deng Z Brady B Xia N Zhou Y Shi H
author_facet	Gao L Li Q Deng Z Brady B Xia N Zhou Y Shi H
author_sort	Gao L
title	Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy
title_short	Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy
title_full	Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy
title_fullStr	Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy
title_full_unstemmed	Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy
title_sort	highly sensitive protein detection via covalently linked aptamer to mos2 and exonuclease-assisted amplification strategy
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/a322089f8bb14671b099b3629f98123d
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Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assisted amplification strategy

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