AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.

The MUC2 mucin is the major constituent of the two mucus layers in colon. Mice lacking the disulfide isomerase-like protein Agr2 have been shown to be more susceptible to colon inflammation. The Agr2(-/-) mice have less filled goblet cells and were now shown to have a poorly developed inner colon mu...

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Autores principales:	Joakim H Bergström, Katarina A Berg, Ana M Rodríguez-Piñeiro, Bärbel Stecher, Malin E V Johansson, Gunnar C Hansson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a32a76d0146a45258a5f10e12b382c2e
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spelling	oai:doaj.org-article:a32a76d0146a45258a5f10e12b382c2e2021-11-25T06:05:16ZAGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.1932-620310.1371/journal.pone.0104186https://doaj.org/article/a32a76d0146a45258a5f10e12b382c2e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25111734/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The MUC2 mucin is the major constituent of the two mucus layers in colon. Mice lacking the disulfide isomerase-like protein Agr2 have been shown to be more susceptible to colon inflammation. The Agr2(-/-) mice have less filled goblet cells and were now shown to have a poorly developed inner colon mucus layer. We could not show AGR2 covalently bound to recombinant MUC2 N- and C-termini as have previously been suggested. We found relatively high concentrations of Agr2 in secreted mucus throughout the murine gastrointestinal tract, suggesting that Agr2 may play extracellular roles. In tissue culture (CHO-K1) cells, AGR2 is normally not secreted. Replacement of the single Cys in AGR2 with Ser (C81S) allowed secretion, suggesting that modification of this Cys might provide a mechanism for circumventing the KTEL endoplasmic reticulum retention signal. In conclusion, these results suggest that AGR2 has both intracellular and extracellular effects in the intestine.Joakim H BergströmKatarina A BergAna M Rodríguez-PiñeiroBärbel StecherMalin E V JohanssonGunnar C HanssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104186 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joakim H Bergström Katarina A Berg Ana M Rodríguez-Piñeiro Bärbel Stecher Malin E V Johansson Gunnar C Hansson AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.
description	The MUC2 mucin is the major constituent of the two mucus layers in colon. Mice lacking the disulfide isomerase-like protein Agr2 have been shown to be more susceptible to colon inflammation. The Agr2(-/-) mice have less filled goblet cells and were now shown to have a poorly developed inner colon mucus layer. We could not show AGR2 covalently bound to recombinant MUC2 N- and C-termini as have previously been suggested. We found relatively high concentrations of Agr2 in secreted mucus throughout the murine gastrointestinal tract, suggesting that Agr2 may play extracellular roles. In tissue culture (CHO-K1) cells, AGR2 is normally not secreted. Replacement of the single Cys in AGR2 with Ser (C81S) allowed secretion, suggesting that modification of this Cys might provide a mechanism for circumventing the KTEL endoplasmic reticulum retention signal. In conclusion, these results suggest that AGR2 has both intracellular and extracellular effects in the intestine.
format	article
author	Joakim H Bergström Katarina A Berg Ana M Rodríguez-Piñeiro Bärbel Stecher Malin E V Johansson Gunnar C Hansson
author_facet	Joakim H Bergström Katarina A Berg Ana M Rodríguez-Piñeiro Bärbel Stecher Malin E V Johansson Gunnar C Hansson
author_sort	Joakim H Bergström
title	AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.
title_short	AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.
title_full	AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.
title_fullStr	AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.
title_full_unstemmed	AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.
title_sort	agr2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a32a76d0146a45258a5f10e12b382c2e
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AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus.

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