Aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.

Infection with Aspergillus terreus is more likely to result in invasive, disseminated disease when compared to other Aspergillus species; importantly this species appears to be less susceptible to the antifungal drug amphotericin B. Unique to this species is the ability to produce specialized struct...

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Autores principales:	Eszter Deak, Selwyn D Wilson, Elizabeth White, Janice H Carr, S Arunmozhi Balajee
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a32d6b2c272948089858b3d9fe3828c8
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spelling	oai:doaj.org-article:a32d6b2c272948089858b3d9fe3828c82021-11-25T06:28:23ZAspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.1932-620310.1371/journal.pone.0007673https://doaj.org/article/a32d6b2c272948089858b3d9fe3828c82009-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19888344/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Infection with Aspergillus terreus is more likely to result in invasive, disseminated disease when compared to other Aspergillus species; importantly this species appears to be less susceptible to the antifungal drug amphotericin B. Unique to this species is the ability to produce specialized structures denoted as accessory conidia (AC) directly on hyphae both in vitro and in vivo. With the hypothesis that production of AC by A. terreus may enhance virulence of this organism, we analyzed the phenotype, structure and metabolic potential of these conidia. Comparison of A. terreus phialidic conidia (conidia that arise from conidiophores, PC) and AC architecture by electron microscopy revealed distinct morphological differences between the two conidial forms; AC have a smoother, thicker outer cell surface with no apparent pigment-like layer. Further, AC germinated rapidly, had enhanced adherence to microspheres, and were metabolically more active compared to PC. Additionally, AC contained less cell membrane ergosterol, which correlated with decreased susceptibility to AMB as determined using a flow cytometry based analysis. Furthermore, AC exhibited surface patches of beta1-3 glucan, suggestive of attachment scarring. Collectively, the findings of this study suggest a possible role for AC in A. terreus pathogenesis.Eszter DeakSelwyn D WilsonElizabeth WhiteJanice H CarrS Arunmozhi BalajeePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 10, p e7673 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eszter Deak Selwyn D Wilson Elizabeth White Janice H Carr S Arunmozhi Balajee Aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.
description	Infection with Aspergillus terreus is more likely to result in invasive, disseminated disease when compared to other Aspergillus species; importantly this species appears to be less susceptible to the antifungal drug amphotericin B. Unique to this species is the ability to produce specialized structures denoted as accessory conidia (AC) directly on hyphae both in vitro and in vivo. With the hypothesis that production of AC by A. terreus may enhance virulence of this organism, we analyzed the phenotype, structure and metabolic potential of these conidia. Comparison of A. terreus phialidic conidia (conidia that arise from conidiophores, PC) and AC architecture by electron microscopy revealed distinct morphological differences between the two conidial forms; AC have a smoother, thicker outer cell surface with no apparent pigment-like layer. Further, AC germinated rapidly, had enhanced adherence to microspheres, and were metabolically more active compared to PC. Additionally, AC contained less cell membrane ergosterol, which correlated with decreased susceptibility to AMB as determined using a flow cytometry based analysis. Furthermore, AC exhibited surface patches of beta1-3 glucan, suggestive of attachment scarring. Collectively, the findings of this study suggest a possible role for AC in A. terreus pathogenesis.
format	article
author	Eszter Deak Selwyn D Wilson Elizabeth White Janice H Carr S Arunmozhi Balajee
author_facet	Eszter Deak Selwyn D Wilson Elizabeth White Janice H Carr S Arunmozhi Balajee
author_sort	Eszter Deak
title	Aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.
title_short	Aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.
title_full	Aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.
title_fullStr	Aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.
title_full_unstemmed	Aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.
title_sort	aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/a32d6b2c272948089858b3d9fe3828c8
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Aspergillus terreus accessory conidia are unique in surface architecture, cell wall composition and germination kinetics.

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