Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber

Abstract The ratio of type III to type I collagen is important for properly maintaining functions of organs and cells. We propose a method to quantify the ratio of type III to total (type I + III) collagen (λ III ) in a given collagen fiber bundle using second harmonic generation (SHG) light. First,...

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Autores principales:	Shukei Sugita, Takuya Suzumura, Akinobu Nakamura, Shinya Tsukiji, Yoshihiro Ujihara, Masanori Nakamura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a33605cf9ec745b2a13078a81bb2ee8d
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spelling	oai:doaj.org-article:a33605cf9ec745b2a13078a81bb2ee8d2021-12-02T15:56:41ZSecond harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber10.1038/s41598-021-91302-32045-2322https://doaj.org/article/a33605cf9ec745b2a13078a81bb2ee8d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91302-3https://doaj.org/toc/2045-2322Abstract The ratio of type III to type I collagen is important for properly maintaining functions of organs and cells. We propose a method to quantify the ratio of type III to total (type I + III) collagen (λ III ) in a given collagen fiber bundle using second harmonic generation (SHG) light. First, the relationship between SHG light intensity and the λ III of collagen gels was examined, and the slope (k 1) and SHG light intensity at 0% type III collagen (k 2) were determined. Second, the SHG light intensity of a 100% type I collagen fiber bundle and its diameter (D) were measured, and the slope (k 3) of the relationship was determined. The λ III in a collagen fiber bundle was estimated from these constants (k 1-3) and SHG light intensity. We applied this method to collagen fiber bundles isolated from the media and adventitia of porcine thoracic aortas, and obtained λ III = 84.7% ± 13.8% and λ III = 17.5% ± 15.2%, respectively. These values concurred with those obtained with a typical quantification method using sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The findings demonstrated that the method proposed is useful to quantify the ratio of type III to total collagen in a collagen fiber bundle.Shukei SugitaTakuya SuzumuraAkinobu NakamuraShinya TsukijiYoshihiro UjiharaMasanori NakamuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shukei Sugita Takuya Suzumura Akinobu Nakamura Shinya Tsukiji Yoshihiro Ujihara Masanori Nakamura Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber
description	Abstract The ratio of type III to type I collagen is important for properly maintaining functions of organs and cells. We propose a method to quantify the ratio of type III to total (type I + III) collagen (λ III ) in a given collagen fiber bundle using second harmonic generation (SHG) light. First, the relationship between SHG light intensity and the λ III of collagen gels was examined, and the slope (k 1) and SHG light intensity at 0% type III collagen (k 2) were determined. Second, the SHG light intensity of a 100% type I collagen fiber bundle and its diameter (D) were measured, and the slope (k 3) of the relationship was determined. The λ III in a collagen fiber bundle was estimated from these constants (k 1-3) and SHG light intensity. We applied this method to collagen fiber bundles isolated from the media and adventitia of porcine thoracic aortas, and obtained λ III = 84.7% ± 13.8% and λ III = 17.5% ± 15.2%, respectively. These values concurred with those obtained with a typical quantification method using sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The findings demonstrated that the method proposed is useful to quantify the ratio of type III to total collagen in a collagen fiber bundle.
format	article
author	Shukei Sugita Takuya Suzumura Akinobu Nakamura Shinya Tsukiji Yoshihiro Ujihara Masanori Nakamura
author_facet	Shukei Sugita Takuya Suzumura Akinobu Nakamura Shinya Tsukiji Yoshihiro Ujihara Masanori Nakamura
author_sort	Shukei Sugita
title	Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber
title_short	Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber
title_full	Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber
title_fullStr	Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber
title_full_unstemmed	Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber
title_sort	second harmonic generation light quantifies the ratio of type iii to total (i + iii) collagen in a bundle of collagen fiber
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a33605cf9ec745b2a13078a81bb2ee8d
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Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber

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