Interactions of Respiratory Viruses and the Nasal Microbiota during the First Year of Life in Healthy Infants
ABSTRACT Traditional culture techniques have shown that increased bacterial colonization is associated with viral colonization; however, the influence of viral colonization on the whole microbiota composition is less clear. We thus aimed to understand the interaction of viral infections and the nasa...
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American Society for Microbiology
2016
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oai:doaj.org-article:a33b07baf372451b8f688b61e99498b82021-11-15T15:22:03ZInteractions of Respiratory Viruses and the Nasal Microbiota during the First Year of Life in Healthy Infants10.1128/mSphere.00312-162379-5042https://doaj.org/article/a33b07baf372451b8f688b61e99498b82016-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00312-16https://doaj.org/toc/2379-5042ABSTRACT Traditional culture techniques have shown that increased bacterial colonization is associated with viral colonization; however, the influence of viral colonization on the whole microbiota composition is less clear. We thus aimed to understand the interaction of viral infections and the nasal microbiota in early life to appraise their roles in disease development. Thirty-two healthy, unselected infants were included in this prospective longitudinal cohort study within the first year of life. Biweekly nasal swabs (n = 559) were taken, and the microbiota was analyzed by 16S rRNA pyrosequencing, and 10 different viruses and 2 atypical bacteria were characterized by real-time PCR (combination of seven duplex samples). In contrast to asymptomatic human rhinovirus (HRV) colonization, symptomatic HRV infections were associated with lower alpha diversity (Shannon diversity index [SDI]), higher bacterial density (PCR concentration), and a difference in beta diversities (Jaccard and Bray-Curtis index) of the microbiota. In addition, infants with more frequent HRV infections had a lower SDI at the end of the study period. Overall, changes in the microbiota associated with symptomatic HRV infections were characterized by a loss of microbial diversity. The interaction between HRV infections and the nasal microbiota in early life might be of importance for later disease development and indicate a potential approach for future interventions. IMPORTANCE Respiratory viral infections are very frequent in infancy and of importance in acute and chronic disease development. Infections with human rhinovirus (HRV) are, e.g., associated with the later development of asthma. We found that only symptomatic HRV infections were associated with acute changes in the nasal microbiota, mainly characterized by a loss of microbial diversity. Infants with more frequent symptomatic HRV infections had a lower bacterial diversity at the end of the first year of life. Whether the interaction between viruses and the microbiota is one pathway contributing to asthma development will be assessed in the follow-ups of these children. Independent of that, measurements of microbial diversity might represent a potential marker for risk of later lung disease or monitoring of early life interventions.Insa KortenMoana MikaShkipe KlenjaElisabeth KieningerInes MackMaria Teresa BarbaniMeri GorgievskiUrs FreyMarkus HiltyPhilipp LatzinAmerican Society for Microbiologyarticlebacteriologyhuman rhinovirusmicrobiotapediatric infectious diseaserespiratory virusesMicrobiologyQR1-502ENmSphere, Vol 1, Iss 6 (2016) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
bacteriology human rhinovirus microbiota pediatric infectious disease respiratory viruses Microbiology QR1-502 |
| spellingShingle |
bacteriology human rhinovirus microbiota pediatric infectious disease respiratory viruses Microbiology QR1-502 Insa Korten Moana Mika Shkipe Klenja Elisabeth Kieninger Ines Mack Maria Teresa Barbani Meri Gorgievski Urs Frey Markus Hilty Philipp Latzin Interactions of Respiratory Viruses and the Nasal Microbiota during the First Year of Life in Healthy Infants |
| description |
ABSTRACT Traditional culture techniques have shown that increased bacterial colonization is associated with viral colonization; however, the influence of viral colonization on the whole microbiota composition is less clear. We thus aimed to understand the interaction of viral infections and the nasal microbiota in early life to appraise their roles in disease development. Thirty-two healthy, unselected infants were included in this prospective longitudinal cohort study within the first year of life. Biweekly nasal swabs (n = 559) were taken, and the microbiota was analyzed by 16S rRNA pyrosequencing, and 10 different viruses and 2 atypical bacteria were characterized by real-time PCR (combination of seven duplex samples). In contrast to asymptomatic human rhinovirus (HRV) colonization, symptomatic HRV infections were associated with lower alpha diversity (Shannon diversity index [SDI]), higher bacterial density (PCR concentration), and a difference in beta diversities (Jaccard and Bray-Curtis index) of the microbiota. In addition, infants with more frequent HRV infections had a lower SDI at the end of the study period. Overall, changes in the microbiota associated with symptomatic HRV infections were characterized by a loss of microbial diversity. The interaction between HRV infections and the nasal microbiota in early life might be of importance for later disease development and indicate a potential approach for future interventions. IMPORTANCE Respiratory viral infections are very frequent in infancy and of importance in acute and chronic disease development. Infections with human rhinovirus (HRV) are, e.g., associated with the later development of asthma. We found that only symptomatic HRV infections were associated with acute changes in the nasal microbiota, mainly characterized by a loss of microbial diversity. Infants with more frequent symptomatic HRV infections had a lower bacterial diversity at the end of the first year of life. Whether the interaction between viruses and the microbiota is one pathway contributing to asthma development will be assessed in the follow-ups of these children. Independent of that, measurements of microbial diversity might represent a potential marker for risk of later lung disease or monitoring of early life interventions. |
| format |
article |
| author |
Insa Korten Moana Mika Shkipe Klenja Elisabeth Kieninger Ines Mack Maria Teresa Barbani Meri Gorgievski Urs Frey Markus Hilty Philipp Latzin |
| author_facet |
Insa Korten Moana Mika Shkipe Klenja Elisabeth Kieninger Ines Mack Maria Teresa Barbani Meri Gorgievski Urs Frey Markus Hilty Philipp Latzin |
| author_sort |
Insa Korten |
| title |
Interactions of Respiratory Viruses and the Nasal Microbiota during the First Year of Life in Healthy Infants |
| title_short |
Interactions of Respiratory Viruses and the Nasal Microbiota during the First Year of Life in Healthy Infants |
| title_full |
Interactions of Respiratory Viruses and the Nasal Microbiota during the First Year of Life in Healthy Infants |
| title_fullStr |
Interactions of Respiratory Viruses and the Nasal Microbiota during the First Year of Life in Healthy Infants |
| title_full_unstemmed |
Interactions of Respiratory Viruses and the Nasal Microbiota during the First Year of Life in Healthy Infants |
| title_sort |
interactions of respiratory viruses and the nasal microbiota during the first year of life in healthy infants |
| publisher |
American Society for Microbiology |
| publishDate |
2016 |
| url |
https://doaj.org/article/a33b07baf372451b8f688b61e99498b8 |
| work_keys_str_mv |
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