Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells

Abstract The authors' previous study showed that zirconium oxide nanoparticles (ZrO2 NPs) induce toxic effects in MC3T3‐E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time‐of‐flight mass spectrometry was used to reveal the metabolite pro...

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Autores principales:	Mingfu Ye, Linhu Wang, Zhang Wu, Wenjun Liu
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	biomarkers lysoPCs lysoPEs metabolite profiling ZrO2 nanoparticles Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/a3464963c3874ea8afef8f248ce165e2
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spelling	oai:doaj.org-article:a3464963c3874ea8afef8f248ce165e22021-11-27T07:09:27ZMetabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells1751-875X1751-874110.1049/nbt2.12067https://doaj.org/article/a3464963c3874ea8afef8f248ce165e22021-12-01T00:00:00Zhttps://doi.org/10.1049/nbt2.12067https://doaj.org/toc/1751-8741https://doaj.org/toc/1751-875XAbstract The authors' previous study showed that zirconium oxide nanoparticles (ZrO2 NPs) induce toxic effects in MC3T3‐E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time‐of‐flight mass spectrometry was used to reveal the metabolite profile and toxicological mechanism of MC3T3‐E1 cells in response to ZrO2 NPs. The results demonstrated that MC3T3‐E1 cells treated with ZrO2 NPs for 24 and 48 h presented different metabolic characteristics. Following ZrO2 NP treatment for 24 h, 96 upregulated and 129 downregulated metabolites in the positive ion mode, as well as 91 upregulated and 326 downregulated metabolites in the negative ion mode were identified. Following ZrO2 NP treatment for 48 h, 33 upregulated and 174 downregulated metabolites were identified in the positive ion mode, whereas 37 upregulated and 302 downregulated metabolites were confirmed in the negative ion mode. Among them, 42 differential metabolites were recognised as potential metabolites contributing to the induced toxic effects of ZrO2 NPs in MC3T3‐E1 cells. Most of the differential metabolites were lysophosphatidylcholine and lysophosphatidylethanolamide, indicating that exposure to ZrO2 NPs may have a profound impact on human cellular function by impairing the membrane system. The results also provide new clues for the toxicological mechanism of ZrO2 NP dental materials.Mingfu YeLinhu WangZhang WuWenjun LiuWileyarticlebiomarkerslysoPCslysoPEsmetabolite profilingZrO2 nanoparticlesBiotechnologyTP248.13-248.65ENIET Nanobiotechnology, Vol 15, Iss 9, Pp 687-697 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biomarkers lysoPCs lysoPEs metabolite profiling ZrO2 nanoparticles Biotechnology TP248.13-248.65
spellingShingle	biomarkers lysoPCs lysoPEs metabolite profiling ZrO2 nanoparticles Biotechnology TP248.13-248.65 Mingfu Ye Linhu Wang Zhang Wu Wenjun Liu Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells
description	Abstract The authors' previous study showed that zirconium oxide nanoparticles (ZrO2 NPs) induce toxic effects in MC3T3‐E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time‐of‐flight mass spectrometry was used to reveal the metabolite profile and toxicological mechanism of MC3T3‐E1 cells in response to ZrO2 NPs. The results demonstrated that MC3T3‐E1 cells treated with ZrO2 NPs for 24 and 48 h presented different metabolic characteristics. Following ZrO2 NP treatment for 24 h, 96 upregulated and 129 downregulated metabolites in the positive ion mode, as well as 91 upregulated and 326 downregulated metabolites in the negative ion mode were identified. Following ZrO2 NP treatment for 48 h, 33 upregulated and 174 downregulated metabolites were identified in the positive ion mode, whereas 37 upregulated and 302 downregulated metabolites were confirmed in the negative ion mode. Among them, 42 differential metabolites were recognised as potential metabolites contributing to the induced toxic effects of ZrO2 NPs in MC3T3‐E1 cells. Most of the differential metabolites were lysophosphatidylcholine and lysophosphatidylethanolamide, indicating that exposure to ZrO2 NPs may have a profound impact on human cellular function by impairing the membrane system. The results also provide new clues for the toxicological mechanism of ZrO2 NP dental materials.
format	article
author	Mingfu Ye Linhu Wang Zhang Wu Wenjun Liu
author_facet	Mingfu Ye Linhu Wang Zhang Wu Wenjun Liu
author_sort	Mingfu Ye
title	Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells
title_short	Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells
title_full	Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells
title_fullStr	Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells
title_full_unstemmed	Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells
title_sort	metabolomic profiling of zro2 nanoparticles in mc3t3‐e1 cells
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/a3464963c3874ea8afef8f248ce165e2
work_keys_str_mv	AT mingfuye metabolomicprofilingofzro2nanoparticlesinmc3t3e1cells AT linhuwang metabolomicprofilingofzro2nanoparticlesinmc3t3e1cells AT zhangwu metabolomicprofilingofzro2nanoparticlesinmc3t3e1cells AT wenjunliu metabolomicprofilingofzro2nanoparticlesinmc3t3e1cells
_version_	1718409179876556800

Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells

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