Genome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms

ABSTRACT Salmonella enterica subsp. enterica bacteria are important foodborne pathogens with major economic impact. Some isolates exhibit increased heat tolerance, a concern for food safety. Analysis of a finished-quality genome sequence of an isolate commonly used in heat resistance studies, S. ent...

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Autores principales:	Scott V. Nguyen, Gregory P. Harhay, James L. Bono, Timothy P. L. Smith, Dayna M. Harhay
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	IncHI2 Salmonella genome analysis phylogenetic analysis thermotolerance Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a348905fbdca491794e34872cdd06c7e
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spelling	oai:doaj.org-article:a348905fbdca491794e34872cdd06c7e2021-12-02T18:15:43ZGenome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms10.1128/mSystems.00190-162379-5077https://doaj.org/article/a348905fbdca491794e34872cdd06c7e2017-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00190-16https://doaj.org/toc/2379-5077ABSTRACT Salmonella enterica subsp. enterica bacteria are important foodborne pathogens with major economic impact. Some isolates exhibit increased heat tolerance, a concern for food safety. Analysis of a finished-quality genome sequence of an isolate commonly used in heat resistance studies, S. enterica subsp. enterica serovar Senftenberg 775W (ATCC 43845), demonstrated an interesting observation that this strain contains not just one, but two horizontally acquired thermotolerance locus homologs. These two loci reside on a large 341.3-kbp plasmid that is similar to the well-studied IncHI2 R478 plasmid but lacks any antibiotic resistance genes found on R478 or other IncHI2 plasmids. As this historical Salmonella isolate has been in use since 1941, comparative analysis of the plasmid and of the thermotolerance loci contained on the plasmid will provide insight into the evolution of heat resistance loci as well as acquisition of resistance determinants in IncHI2 plasmids. IMPORTANCE Thermal interventions are commonly used in the food industry as a means of mitigating pathogen contamination in food products. Concern over heat-resistant food contaminants has recently increased, with the identification of a conserved locus shown to confer heat resistance in disparate lineages of Gram-negative bacteria. Complete sequence analysis of a historical isolate of Salmonella enterica serovar Senftenberg, used in numerous studies because of its novel heat resistance, revealed that this important strain possesses two distinct copies of this conserved thermotolerance locus, residing on a multireplicon IncHI2/IncHI2A plasmid. Phylogenetic analysis of these loci in comparison with homologs identified in various bacterial genera provides an opportunity to examine the evolution and distribution of loci conferring resistance to environmental stressors, such as heat and desiccation.Scott V. NguyenGregory P. HarhayJames L. BonoTimothy P. L. SmithDayna M. HarhayAmerican Society for MicrobiologyarticleIncHI2Salmonellagenome analysisphylogenetic analysisthermotoleranceMicrobiologyQR1-502ENmSystems, Vol 2, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	IncHI2 Salmonella genome analysis phylogenetic analysis thermotolerance Microbiology QR1-502
spellingShingle	IncHI2 Salmonella genome analysis phylogenetic analysis thermotolerance Microbiology QR1-502 Scott V. Nguyen Gregory P. Harhay James L. Bono Timothy P. L. Smith Dayna M. Harhay Genome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms
description	ABSTRACT Salmonella enterica subsp. enterica bacteria are important foodborne pathogens with major economic impact. Some isolates exhibit increased heat tolerance, a concern for food safety. Analysis of a finished-quality genome sequence of an isolate commonly used in heat resistance studies, S. enterica subsp. enterica serovar Senftenberg 775W (ATCC 43845), demonstrated an interesting observation that this strain contains not just one, but two horizontally acquired thermotolerance locus homologs. These two loci reside on a large 341.3-kbp plasmid that is similar to the well-studied IncHI2 R478 plasmid but lacks any antibiotic resistance genes found on R478 or other IncHI2 plasmids. As this historical Salmonella isolate has been in use since 1941, comparative analysis of the plasmid and of the thermotolerance loci contained on the plasmid will provide insight into the evolution of heat resistance loci as well as acquisition of resistance determinants in IncHI2 plasmids. IMPORTANCE Thermal interventions are commonly used in the food industry as a means of mitigating pathogen contamination in food products. Concern over heat-resistant food contaminants has recently increased, with the identification of a conserved locus shown to confer heat resistance in disparate lineages of Gram-negative bacteria. Complete sequence analysis of a historical isolate of Salmonella enterica serovar Senftenberg, used in numerous studies because of its novel heat resistance, revealed that this important strain possesses two distinct copies of this conserved thermotolerance locus, residing on a multireplicon IncHI2/IncHI2A plasmid. Phylogenetic analysis of these loci in comparison with homologs identified in various bacterial genera provides an opportunity to examine the evolution and distribution of loci conferring resistance to environmental stressors, such as heat and desiccation.
format	article
author	Scott V. Nguyen Gregory P. Harhay James L. Bono Timothy P. L. Smith Dayna M. Harhay
author_facet	Scott V. Nguyen Gregory P. Harhay James L. Bono Timothy P. L. Smith Dayna M. Harhay
author_sort	Scott V. Nguyen
title	Genome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms
title_short	Genome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms
title_full	Genome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms
title_fullStr	Genome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms
title_full_unstemmed	Genome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms
title_sort	genome sequence of the thermotolerant foodborne pathogen <named-content content-type="genus-species">salmonella enterica</named-content> serovar senftenberg atcc 43845 and phylogenetic analysis of loci encoding increased protein quality control mechanisms
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/a348905fbdca491794e34872cdd06c7e
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Genome Sequence of the Thermotolerant Foodborne Pathogen <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Senftenberg ATCC 43845 and Phylogenetic Analysis of Loci Encoding Increased Protein Quality Control Mechanisms

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