Hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress

The excessive production of free radicals causes oxidative damage and degenerative diseases. Whereas, catalase is a macromolecule with a specific activity, but Malondialdehyde (MDA) is a small molecule with no activity. The peels of red dragon fruit (H. polyrhizus) are potential to be natural exogen...

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Autores principales:	SRI WAHDANINGSIH, EKA KARTIKA UNTARI
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Syiah Kuala 2021
Materias:	catalase, h. polyrhizus, mda, oxidative stress Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/a35711922adb4155ace9c85cff010f81
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spelling	oai:doaj.org-article:a35711922adb4155ace9c85cff010f812021-11-18T07:51:52ZHylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress1411-85132541-406210.24815/jn.v21i3.19474https://doaj.org/article/a35711922adb4155ace9c85cff010f812021-10-01T00:00:00Zhttp://jurnal.unsyiah.ac.id/natural/article/view/19474https://doaj.org/toc/1411-8513https://doaj.org/toc/2541-4062The excessive production of free radicals causes oxidative damage and degenerative diseases. Whereas, catalase is a macromolecule with a specific activity, but Malondialdehyde (MDA) is a small molecule with no activity. The peels of red dragon fruit (H. polyrhizus) are potential to be natural exogenous antioxidants. This research aimed to determine the effects of methanol fraction from H. polyrhizus peels on catalase activity and MDA level. The peel was macerated using chloroform and fractionated using methanol. The stress condition was done through five-day fasting and ten-minute swimming per day. The activity of catalase and MDA was measured using UV-Vis spectrophotometer. Thirty five rats were divided into 7 groups: normal, negative, dose I (5 mg/200gBW), dose II (10 mg/200gBW), dose III (20 mg/200gBW), positive control (quercetin) (4 mg/200gBW) and vitamin E (17.64 mg/kg). The activity of catalase in the normal, negative, vitamin E, and quercetin, dose I, II, and II respectively were: 28.37; 7.8; 24.85; 34.33; 59.92; 28.18; and 70.85 Unit/mL. The average MDA level in the groups respectively were: 0.042; 0.051; 0.034; 0.042; 0.037; 0.033; 0.030 μg/mL. The result of One Way ANOVA test showed that the catalase activity in dose III was significantly different than normal group; the MDA levels in all groups were significantly different than normal group. This research revealed that methanol fraction from H. polyrhizus peels is a potent antioxidant for its capacity to increase the activity of catalase and reduce the MDA level in rats with oxidative stress.SRI WAHDANINGSIHEKA KARTIKA UNTARIUniversitas Syiah Kualaarticlecatalase, h. polyrhizus, mda, oxidative stressScience (General)Q1-390ENJurnal Natural, Vol 21, Iss 3, Pp 150-159 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	catalase, h. polyrhizus, mda, oxidative stress Science (General) Q1-390
spellingShingle	catalase, h. polyrhizus, mda, oxidative stress Science (General) Q1-390 SRI WAHDANINGSIH EKA KARTIKA UNTARI Hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress
description	The excessive production of free radicals causes oxidative damage and degenerative diseases. Whereas, catalase is a macromolecule with a specific activity, but Malondialdehyde (MDA) is a small molecule with no activity. The peels of red dragon fruit (H. polyrhizus) are potential to be natural exogenous antioxidants. This research aimed to determine the effects of methanol fraction from H. polyrhizus peels on catalase activity and MDA level. The peel was macerated using chloroform and fractionated using methanol. The stress condition was done through five-day fasting and ten-minute swimming per day. The activity of catalase and MDA was measured using UV-Vis spectrophotometer. Thirty five rats were divided into 7 groups: normal, negative, dose I (5 mg/200gBW), dose II (10 mg/200gBW), dose III (20 mg/200gBW), positive control (quercetin) (4 mg/200gBW) and vitamin E (17.64 mg/kg). The activity of catalase in the normal, negative, vitamin E, and quercetin, dose I, II, and II respectively were: 28.37; 7.8; 24.85; 34.33; 59.92; 28.18; and 70.85 Unit/mL. The average MDA level in the groups respectively were: 0.042; 0.051; 0.034; 0.042; 0.037; 0.033; 0.030 μg/mL. The result of One Way ANOVA test showed that the catalase activity in dose III was significantly different than normal group; the MDA levels in all groups were significantly different than normal group. This research revealed that methanol fraction from H. polyrhizus peels is a potent antioxidant for its capacity to increase the activity of catalase and reduce the MDA level in rats with oxidative stress.
format	article
author	SRI WAHDANINGSIH EKA KARTIKA UNTARI
author_facet	SRI WAHDANINGSIH EKA KARTIKA UNTARI
author_sort	SRI WAHDANINGSIH
title	Hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress
title_short	Hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress
title_full	Hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress
title_fullStr	Hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress
title_full_unstemmed	Hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress
title_sort	hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress
publisher	Universitas Syiah Kuala
publishDate	2021
url	https://doaj.org/article/a35711922adb4155ace9c85cff010f81
work_keys_str_mv	AT sriwahdaningsih hylocereuspolyrhizuseffectoncatalaseandmalondialdehydeinratswithoxidativestress AT ekakartikauntari hylocereuspolyrhizuseffectoncatalaseandmalondialdehydeinratswithoxidativestress
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Hylocereus polyrhizus effect on catalase and malondialdehyde in rats with oxidative stress

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