Anticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway

Abstract This study aimed to investigate the anticancer activity of dried-pericarp water extract of fermented C. japonicus (CJ). The dried-pericarp water extracts of CJ were fermented using Aspergillus oryzae and Saccharomyces cerevisiae at 30 °C and 35 °C. The anticancer activities of both water ex...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Eugene Cho, Jin Kim, Da Hye Jeong, Hyoun Woo Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a358a2beba6842e4a3342fc29f661195
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a358a2beba6842e4a3342fc29f661195
record_format	dspace
spelling	oai:doaj.org-article:a358a2beba6842e4a3342fc29f6611952021-11-08T10:53:32ZAnticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway10.1038/s41598-021-01127-32045-2322https://doaj.org/article/a358a2beba6842e4a3342fc29f6611952021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01127-3https://doaj.org/toc/2045-2322Abstract This study aimed to investigate the anticancer activity of dried-pericarp water extract of fermented C. japonicus (CJ). The dried-pericarp water extracts of CJ were fermented using Aspergillus oryzae and Saccharomyces cerevisiae at 30 °C and 35 °C. The anticancer activities of both water extracts fermented at 30 °C and 35 °C using A. oryzae against FaDu cells were remarkably changed compared with unfermented dried-pericarp water extract of CJ, which has no anticancer activity. Cleaved-PARP, caspase 3, and apoptotic cells stained with annexin V/PI were significantly increased by treatment with A. oryzae extracts fermented at 30 °C. The insulin-like growth factor-binding protein 2 (IGFBP-2) protein level and mTOR phosphorylation by A. oryzae fermented extracts (AOFE) were dramatically reduced, and the expression levels of IGFBP-2 and phosphorylated mTOR were significantly increased depending on the glucose concentrations in FaDu cells. These results suggested that the cell viabilities in AOFE were restored as the glucose concentrations increased. Furthermore, it was confirmed LC/MS/MS that the content of gallic acid was increased by fermentation of Aspergillus oryzae (5.596 ± 0.1746 μg/mg) compared to the unfermented extract (1.620 ± 0.0432 μg/mg). Based on these results, the anticancer effect of AOFE was achieved through inhibition of the IGFBP-2/mTOR signaling pathway. These results suggest that AOFE may be a potential treatment for head and neck cancer.Eugene ChoJin KimDa Hye JeongHyoun Woo KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eugene Cho Jin Kim Da Hye Jeong Hyoun Woo Kim Anticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway
description	Abstract This study aimed to investigate the anticancer activity of dried-pericarp water extract of fermented C. japonicus (CJ). The dried-pericarp water extracts of CJ were fermented using Aspergillus oryzae and Saccharomyces cerevisiae at 30 °C and 35 °C. The anticancer activities of both water extracts fermented at 30 °C and 35 °C using A. oryzae against FaDu cells were remarkably changed compared with unfermented dried-pericarp water extract of CJ, which has no anticancer activity. Cleaved-PARP, caspase 3, and apoptotic cells stained with annexin V/PI were significantly increased by treatment with A. oryzae extracts fermented at 30 °C. The insulin-like growth factor-binding protein 2 (IGFBP-2) protein level and mTOR phosphorylation by A. oryzae fermented extracts (AOFE) were dramatically reduced, and the expression levels of IGFBP-2 and phosphorylated mTOR were significantly increased depending on the glucose concentrations in FaDu cells. These results suggested that the cell viabilities in AOFE were restored as the glucose concentrations increased. Furthermore, it was confirmed LC/MS/MS that the content of gallic acid was increased by fermentation of Aspergillus oryzae (5.596 ± 0.1746 μg/mg) compared to the unfermented extract (1.620 ± 0.0432 μg/mg). Based on these results, the anticancer effect of AOFE was achieved through inhibition of the IGFBP-2/mTOR signaling pathway. These results suggest that AOFE may be a potential treatment for head and neck cancer.
format	article
author	Eugene Cho Jin Kim Da Hye Jeong Hyoun Woo Kim
author_facet	Eugene Cho Jin Kim Da Hye Jeong Hyoun Woo Kim
author_sort	Eugene Cho
title	Anticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway
title_short	Anticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway
title_full	Anticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway
title_fullStr	Anticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway
title_full_unstemmed	Anticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway
title_sort	anticancer properties of dried-pericarp water extracts of camellia japonica l. fermented with aspergillus oryzae through regulation of igfbp-2/mtor pathway
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a358a2beba6842e4a3342fc29f661195
work_keys_str_mv	AT eugenecho anticancerpropertiesofdriedpericarpwaterextractsofcamelliajaponicalfermentedwithaspergillusoryzaethroughregulationofigfbp2mtorpathway AT jinkim anticancerpropertiesofdriedpericarpwaterextractsofcamelliajaponicalfermentedwithaspergillusoryzaethroughregulationofigfbp2mtorpathway AT dahyejeong anticancerpropertiesofdriedpericarpwaterextractsofcamelliajaponicalfermentedwithaspergillusoryzaethroughregulationofigfbp2mtorpathway AT hyounwookim anticancerpropertiesofdriedpericarpwaterextractsofcamelliajaponicalfermentedwithaspergillusoryzaethroughregulationofigfbp2mtorpathway
_version_	1718442554016399360

Anticancer properties of dried-pericarp water extracts of Camellia japonica L. fermented with Aspergillus oryzae through regulation of IGFBP-2/mTOR pathway

Ejemplares similares