Discovery and characterization of the feline miRNAome

Abstract The domestic cat is an important human companion animal that can also serve as a relevant model for ~250 genetic diseases, many metabolic and degenerative conditions, and forms of cancer that are analogous to human disorders. MicroRNAs (miRNAs) play a crucial role in many biological process...

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Autores principales:	Alessandro Laganà, Wessel P. Dirksen, Wachiraphan Supsavhad, Ayse Selen Yilmaz, Hatice G. Ozer, James D. Feller, Kiersten A. Vala, Carlo M. Croce, Thomas J. Rosol
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a35aa02febf64e2fa1fefe717a33e022
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spelling	oai:doaj.org-article:a35aa02febf64e2fa1fefe717a33e0222021-12-02T12:32:12ZDiscovery and characterization of the feline miRNAome10.1038/s41598-017-10164-w2045-2322https://doaj.org/article/a35aa02febf64e2fa1fefe717a33e0222017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10164-whttps://doaj.org/toc/2045-2322Abstract The domestic cat is an important human companion animal that can also serve as a relevant model for ~250 genetic diseases, many metabolic and degenerative conditions, and forms of cancer that are analogous to human disorders. MicroRNAs (miRNAs) play a crucial role in many biological processes and their dysregulation has a significant impact on important cellular pathways and is linked to a variety of diseases. While many species already have a well-defined and characterized miRNAome, miRNAs have not been carefully studied in cats. As a result, there are no feline miRNAs present in the reference miRNA databases, diminishing the usefulness of medical research on spontaneous disease in cats for applicability to both feline and human disease. This study was undertaken to define and characterize the cat miRNAome in normal feline tissues. High-throughput sequencing was performed on 12 different normal cat tissues. 271 candidate feline miRNA precursors, encoding a total of 475 mature sequences, were identified, including several novel cat-specific miRNAs. Several analyses were performed to characterize the discovered miRNAs, including tissue distribution of the precursors and mature sequences, genomic distribution of miRNA genes and identification of clusters, and isomiR characterization. Many of the miRNAs were regulated in a tissue/organ-specific manner.Alessandro LaganàWessel P. DirksenWachiraphan SupsavhadAyse Selen YilmazHatice G. OzerJames D. FellerKiersten A. ValaCarlo M. CroceThomas J. RosolNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alessandro Laganà Wessel P. Dirksen Wachiraphan Supsavhad Ayse Selen Yilmaz Hatice G. Ozer James D. Feller Kiersten A. Vala Carlo M. Croce Thomas J. Rosol Discovery and characterization of the feline miRNAome
description	Abstract The domestic cat is an important human companion animal that can also serve as a relevant model for ~250 genetic diseases, many metabolic and degenerative conditions, and forms of cancer that are analogous to human disorders. MicroRNAs (miRNAs) play a crucial role in many biological processes and their dysregulation has a significant impact on important cellular pathways and is linked to a variety of diseases. While many species already have a well-defined and characterized miRNAome, miRNAs have not been carefully studied in cats. As a result, there are no feline miRNAs present in the reference miRNA databases, diminishing the usefulness of medical research on spontaneous disease in cats for applicability to both feline and human disease. This study was undertaken to define and characterize the cat miRNAome in normal feline tissues. High-throughput sequencing was performed on 12 different normal cat tissues. 271 candidate feline miRNA precursors, encoding a total of 475 mature sequences, were identified, including several novel cat-specific miRNAs. Several analyses were performed to characterize the discovered miRNAs, including tissue distribution of the precursors and mature sequences, genomic distribution of miRNA genes and identification of clusters, and isomiR characterization. Many of the miRNAs were regulated in a tissue/organ-specific manner.
format	article
author	Alessandro Laganà Wessel P. Dirksen Wachiraphan Supsavhad Ayse Selen Yilmaz Hatice G. Ozer James D. Feller Kiersten A. Vala Carlo M. Croce Thomas J. Rosol
author_facet	Alessandro Laganà Wessel P. Dirksen Wachiraphan Supsavhad Ayse Selen Yilmaz Hatice G. Ozer James D. Feller Kiersten A. Vala Carlo M. Croce Thomas J. Rosol
author_sort	Alessandro Laganà
title	Discovery and characterization of the feline miRNAome
title_short	Discovery and characterization of the feline miRNAome
title_full	Discovery and characterization of the feline miRNAome
title_fullStr	Discovery and characterization of the feline miRNAome
title_full_unstemmed	Discovery and characterization of the feline miRNAome
title_sort	discovery and characterization of the feline mirnaome
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a35aa02febf64e2fa1fefe717a33e022
work_keys_str_mv	AT alessandrolagana discoveryandcharacterizationofthefelinemirnaome AT wesselpdirksen discoveryandcharacterizationofthefelinemirnaome AT wachiraphansupsavhad discoveryandcharacterizationofthefelinemirnaome AT ayseselenyilmaz discoveryandcharacterizationofthefelinemirnaome AT haticegozer discoveryandcharacterizationofthefelinemirnaome AT jamesdfeller discoveryandcharacterizationofthefelinemirnaome AT kierstenavala discoveryandcharacterizationofthefelinemirnaome AT carlomcroce discoveryandcharacterizationofthefelinemirnaome AT thomasjrosol discoveryandcharacterizationofthefelinemirnaome
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Discovery and characterization of the feline miRNAome

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