Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes

Abstract The current study investigates the decolorization of Indanthrene Blue RS dye and the optimization of process parameters needed for effective decolorization by the bacterial consortium. The pure culture of strain TS8, PMS, and NCH has been isolated from the textile wastewater sample collecte...

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Autores principales:	Swati Sambita Mohanty, Arvind Kumar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a35afac79e584f0b92666117100c7ec3
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spelling	oai:doaj.org-article:a35afac79e584f0b92666117100c7ec32021-12-02T14:21:21ZEnhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes10.1038/s41598-021-87227-62045-2322https://doaj.org/article/a35afac79e584f0b92666117100c7ec32021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87227-6https://doaj.org/toc/2045-2322Abstract The current study investigates the decolorization of Indanthrene Blue RS dye and the optimization of process parameters needed for effective decolorization by the bacterial consortium. The pure culture of strain TS8, PMS, and NCH has been isolated from the textile wastewater sample collected from local textile processing units outlet and dye contaminated soil from Odisha, India. A bacterial consortium-BP of Bacillus flexus TS8 (BF), Proteus mirabilis PMS (PM), and Pseudomonas aeruginosa NCH (PA) were developed. The physicochemical parameters were optimized to attain maximum decolorization efficacy. Degradation of Indanthrene Blue RS and the formation of metabolites were confirmed through UV–vis spectroscopy, FT-IR, and GC–MS analysis. The developed consortium-BP showed an enhanced decolorization of Indanthrene Blue RS dye with an Average decolorization rate of 11,088 µg h−1 within 9 h compared to the individual strains under aerobic conditions. The supplementation of agricultural residual wastes showed increased decolorization efficiency of consortium-BP. Higher reduction in TOC and COD removal (≥ 80%) determined the mineralization of Indanthrene Blue RS by consortium-BP. Significant induction of various oxidoreductive enzymes in consortium-BP compared to that of Individual strains indicates their involvement in the overall decolorization and degradation process, with the higher protein concentration in the intracellular enzymes. Studies on the phytotoxicity effect revealed the non-toxic nature of the degraded products formed on mineralization of Indanthrene Blue RS by consortium-BP. This study represents a new approach for enhanced biodegradation using consortium-BP in treating textile wastewaters containing anthraquinone dyes.Swati Sambita MohantyArvind KumarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Swati Sambita Mohanty Arvind Kumar Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes
description	Abstract The current study investigates the decolorization of Indanthrene Blue RS dye and the optimization of process parameters needed for effective decolorization by the bacterial consortium. The pure culture of strain TS8, PMS, and NCH has been isolated from the textile wastewater sample collected from local textile processing units outlet and dye contaminated soil from Odisha, India. A bacterial consortium-BP of Bacillus flexus TS8 (BF), Proteus mirabilis PMS (PM), and Pseudomonas aeruginosa NCH (PA) were developed. The physicochemical parameters were optimized to attain maximum decolorization efficacy. Degradation of Indanthrene Blue RS and the formation of metabolites were confirmed through UV–vis spectroscopy, FT-IR, and GC–MS analysis. The developed consortium-BP showed an enhanced decolorization of Indanthrene Blue RS dye with an Average decolorization rate of 11,088 µg h−1 within 9 h compared to the individual strains under aerobic conditions. The supplementation of agricultural residual wastes showed increased decolorization efficiency of consortium-BP. Higher reduction in TOC and COD removal (≥ 80%) determined the mineralization of Indanthrene Blue RS by consortium-BP. Significant induction of various oxidoreductive enzymes in consortium-BP compared to that of Individual strains indicates their involvement in the overall decolorization and degradation process, with the higher protein concentration in the intracellular enzymes. Studies on the phytotoxicity effect revealed the non-toxic nature of the degraded products formed on mineralization of Indanthrene Blue RS by consortium-BP. This study represents a new approach for enhanced biodegradation using consortium-BP in treating textile wastewaters containing anthraquinone dyes.
format	article
author	Swati Sambita Mohanty Arvind Kumar
author_facet	Swati Sambita Mohanty Arvind Kumar
author_sort	Swati Sambita Mohanty
title	Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes
title_short	Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes
title_full	Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes
title_fullStr	Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes
title_full_unstemmed	Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes
title_sort	enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a35afac79e584f0b92666117100c7ec3
work_keys_str_mv	AT swatisambitamohanty enhanceddegradationofanthraquinonedyesbymicrobialmonocultureanddevelopedconsortiumthroughtheproductionofspecificenzymes AT arvindkumar enhanceddegradationofanthraquinonedyesbymicrobialmonocultureanddevelopedconsortiumthroughtheproductionofspecificenzymes
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Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes

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