Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling

Abstract Background H9N2 avian influenza viruses (AIVs) infect avian and mammalian hosts and provide internal genes for new emerging highly pathogenic avian viruses that cause severe pneumonia with high mortality, for which few medications are available. Arctiin, a bioactive lignan glycoside, has be...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Beixian Zhou, Linxin Wang, Yueyun Liang, Jing Li, Xiping Pan
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Arctium lappa L. lignan arctiin H9N2 avian influenza virus anti-inflammatory Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/a367426a17554d75a03f81be05323036
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a367426a17554d75a03f81be05323036
record_format	dspace
spelling	oai:doaj.org-article:a367426a17554d75a03f81be053230362021-11-28T12:37:34ZArctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling10.1186/s12906-021-03462-42662-7671https://doaj.org/article/a367426a17554d75a03f81be053230362021-11-01T00:00:00Zhttps://doi.org/10.1186/s12906-021-03462-4https://doaj.org/toc/2662-7671Abstract Background H9N2 avian influenza viruses (AIVs) infect avian and mammalian hosts and provide internal genes for new emerging highly pathogenic avian viruses that cause severe pneumonia with high mortality, for which few medications are available. Arctiin, a bioactive lignan glycoside, has been reported to possess multiple pharmacological properties. However, the effect of arctiin on H9N2 virus infection is unclear. In the current study, we analyzed the effect of arctiin on H9N2 virus infection and the underlying molecular mechanism in vitro. Methods The antiviral effect against H9N2 virus was determined by plaque reduction assay (PRA) and progeny virus reduction assay. We employed MTT assay, qRT-PCR, ELISA, immunofluorescence and Western blotting to better understand the anti-inflammatory effect and corresponding mechanism of arctiin on H9N2 virus-infected cells. Results The results showed that arctiin had antiviral activity against H9N2 virus. Arctiin treatment reduced H9N2 virus-triggered proinflammatory cytokines, such as IL-6, and TNF-α. Moreover, arctiin significantly suppressed H9N2 virus-mediated expression of COX-2 and PGE2. Furthermore, we found that arctiin inhibited H9N2 virus-mediated activation of RIG-I/JNK MAPK signaling. Interestingly, arctiin treatment obviously reversed H9N2 virus-induced reduction of Nrf2, increased the nuclear translocation of Nrf2, and upregulated Nrf2 signaling target genes (HO-1 and SOD2). Zinc protoporphyrin (Znpp)—an HO-1 inhibitor—weakened the inhibitory effect of arctiin on H9N2 virus-induced RIG-I/JNK MAPK and proinflammatory mediators. Conclusion Taken together, these results suggested that the anti-inflammatory effects of arctiin on H9N2 virus infection may be due to the activation of Nrf2/HO-1 and blocked RIG-I/JNK MAPK signaling; thus, arctiin may be a promising agent for prevention and treatment of H9N2 virus infections.Beixian ZhouLinxin WangYueyun LiangJing LiXiping PanBMCarticleArctium lappa L.lignanarctiinH9N2 avian influenza virusanti-inflammatoryOther systems of medicineRZ201-999ENBMC Complementary Medicine and Therapies, Vol 21, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctium lappa L. lignan arctiin H9N2 avian influenza virus anti-inflammatory Other systems of medicine RZ201-999
spellingShingle	Arctium lappa L. lignan arctiin H9N2 avian influenza virus anti-inflammatory Other systems of medicine RZ201-999 Beixian Zhou Linxin Wang Yueyun Liang Jing Li Xiping Pan Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling
description	Abstract Background H9N2 avian influenza viruses (AIVs) infect avian and mammalian hosts and provide internal genes for new emerging highly pathogenic avian viruses that cause severe pneumonia with high mortality, for which few medications are available. Arctiin, a bioactive lignan glycoside, has been reported to possess multiple pharmacological properties. However, the effect of arctiin on H9N2 virus infection is unclear. In the current study, we analyzed the effect of arctiin on H9N2 virus infection and the underlying molecular mechanism in vitro. Methods The antiviral effect against H9N2 virus was determined by plaque reduction assay (PRA) and progeny virus reduction assay. We employed MTT assay, qRT-PCR, ELISA, immunofluorescence and Western blotting to better understand the anti-inflammatory effect and corresponding mechanism of arctiin on H9N2 virus-infected cells. Results The results showed that arctiin had antiviral activity against H9N2 virus. Arctiin treatment reduced H9N2 virus-triggered proinflammatory cytokines, such as IL-6, and TNF-α. Moreover, arctiin significantly suppressed H9N2 virus-mediated expression of COX-2 and PGE2. Furthermore, we found that arctiin inhibited H9N2 virus-mediated activation of RIG-I/JNK MAPK signaling. Interestingly, arctiin treatment obviously reversed H9N2 virus-induced reduction of Nrf2, increased the nuclear translocation of Nrf2, and upregulated Nrf2 signaling target genes (HO-1 and SOD2). Zinc protoporphyrin (Znpp)—an HO-1 inhibitor—weakened the inhibitory effect of arctiin on H9N2 virus-induced RIG-I/JNK MAPK and proinflammatory mediators. Conclusion Taken together, these results suggested that the anti-inflammatory effects of arctiin on H9N2 virus infection may be due to the activation of Nrf2/HO-1 and blocked RIG-I/JNK MAPK signaling; thus, arctiin may be a promising agent for prevention and treatment of H9N2 virus infections.
format	article
author	Beixian Zhou Linxin Wang Yueyun Liang Jing Li Xiping Pan
author_facet	Beixian Zhou Linxin Wang Yueyun Liang Jing Li Xiping Pan
author_sort	Beixian Zhou
title	Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling
title_short	Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling
title_full	Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling
title_fullStr	Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling
title_full_unstemmed	Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling
title_sort	arctiin suppresses h9n2 avian influenza virus-mediated inflammation via activation of nrf2/ho-1 signaling
publisher	BMC
publishDate	2021
url	https://doaj.org/article/a367426a17554d75a03f81be05323036
work_keys_str_mv	AT beixianzhou arctiinsuppressesh9n2avianinfluenzavirusmediatedinflammationviaactivationofnrf2ho1signaling AT linxinwang arctiinsuppressesh9n2avianinfluenzavirusmediatedinflammationviaactivationofnrf2ho1signaling AT yueyunliang arctiinsuppressesh9n2avianinfluenzavirusmediatedinflammationviaactivationofnrf2ho1signaling AT jingli arctiinsuppressesh9n2avianinfluenzavirusmediatedinflammationviaactivationofnrf2ho1signaling AT xipingpan arctiinsuppressesh9n2avianinfluenzavirusmediatedinflammationviaactivationofnrf2ho1signaling
_version_	1718407909384126464

Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling

Ejemplares similares