Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells

Yan-li An, Fang Nie, Zi-yu Wang, Dong-sheng ZhangJiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People&#39;s Republic of ChinaAim: Our objective was to prepare a new nano-sized realgar part...

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Autores principales:	An YL, Nie F, Wang ZY, Zhang DS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a36f8a8ef4044e5e933fcc61d77fcebb
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spelling	oai:doaj.org-article:a36f8a8ef4044e5e933fcc61d77fcebb2021-12-02T00:27:09ZPreparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells1176-91141178-2013https://doaj.org/article/a36f8a8ef4044e5e933fcc61d77fcebb2011-12-01T00:00:00Zhttp://www.dovepress.com/preparation-and-characterization-of-realgar-nanoparticles-and-their-in-a8804https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yan-li An, Fang Nie, Zi-yu Wang, Dong-sheng ZhangJiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People&#39;s Republic of ChinaAim: Our objective was to prepare a new nano-sized realgar particle and characterize its antitumor effect on tumor cells.Methods: Nanoparticles were prepared by coprecipitation and were detected by transmission electron microscopy, scanning electron microscopy, energy dispersive spectrometry (EDS), and dynamic light scattering. An anti-proliferative effect of realgar nanoparticles on rat glioma (C6) cells was determined by the MTT assay. Cell cycle and apoptosis rates were observed by flow cytometry. Apoptosis-related gene expression was detected by immunofluorescence staining.Results: Realgar nanoparticles were successfully prepared. The particles were spherical, with an average diameter of approximately 80 nm, and contained arsenic and sulfur elements. Realgar nanoparticles inhibited C6 cell proliferation and induced apoptosis in a dose- and time-dependent manner. Treatment of C6 cells with realgar nanoparticles significantly increased the proportions of cells in S and G2/M phases, decreased the proportion of cells in G0/G1 phase, downregulated Bcl-2 expression, and substantially upregulated Bax expression.Conclusion: Realgar nanoparticles significantly inhibited C6 glioma cell proliferation and promoted cell apoptosis by inducing the upregulation of Bax and downregulation of Bcl-2 expression. Realgar nanoparticles are a promising in vitro anti-cancer strategy and may be applicable for human cancer therapy studies.Keywords: realgar, preparation, characterization, inhibitory effectAn YLNie FWang ZYZhang DSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3187-3194 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 An YL Nie F Wang ZY Zhang DS Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells
description	Yan-li An, Fang Nie, Zi-yu Wang, Dong-sheng ZhangJiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People&#39;s Republic of ChinaAim: Our objective was to prepare a new nano-sized realgar particle and characterize its antitumor effect on tumor cells.Methods: Nanoparticles were prepared by coprecipitation and were detected by transmission electron microscopy, scanning electron microscopy, energy dispersive spectrometry (EDS), and dynamic light scattering. An anti-proliferative effect of realgar nanoparticles on rat glioma (C6) cells was determined by the MTT assay. Cell cycle and apoptosis rates were observed by flow cytometry. Apoptosis-related gene expression was detected by immunofluorescence staining.Results: Realgar nanoparticles were successfully prepared. The particles were spherical, with an average diameter of approximately 80 nm, and contained arsenic and sulfur elements. Realgar nanoparticles inhibited C6 cell proliferation and induced apoptosis in a dose- and time-dependent manner. Treatment of C6 cells with realgar nanoparticles significantly increased the proportions of cells in S and G2/M phases, decreased the proportion of cells in G0/G1 phase, downregulated Bcl-2 expression, and substantially upregulated Bax expression.Conclusion: Realgar nanoparticles significantly inhibited C6 glioma cell proliferation and promoted cell apoptosis by inducing the upregulation of Bax and downregulation of Bcl-2 expression. Realgar nanoparticles are a promising in vitro anti-cancer strategy and may be applicable for human cancer therapy studies.Keywords: realgar, preparation, characterization, inhibitory effect
format	article
author	An YL Nie F Wang ZY Zhang DS
author_facet	An YL Nie F Wang ZY Zhang DS
author_sort	An YL
title	Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells
title_short	Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells
title_full	Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells
title_fullStr	Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells
title_full_unstemmed	Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells
title_sort	preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/a36f8a8ef4044e5e933fcc61d77fcebb
work_keys_str_mv	AT anyl preparationandcharacterizationofrealgarnanoparticlesandtheirinhibitoryeffectonratgliomacells AT nief preparationandcharacterizationofrealgarnanoparticlesandtheirinhibitoryeffectonratgliomacells AT wangzy preparationandcharacterizationofrealgarnanoparticlesandtheirinhibitoryeffectonratgliomacells AT zhangds preparationandcharacterizationofrealgarnanoparticlesandtheirinhibitoryeffectonratgliomacells
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Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells

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