Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

Abstract Circulating tumor cells (CTCs) detection, enumeration and characterization with microfluidic chips has critical significance in cancer prognosis offering a non-invasive “liquid biopsy”. Based on physical differences of size and deformability, we explore micro-ellipse filters consisting of m...

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Autores principales:	Hongmei Chen, Baoshan Cao, Bo Sun, Yapeng Cao, Ke Yang, Yu-Sheng Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a37053e74e724dea913a4956426993b8
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spelling	oai:doaj.org-article:a37053e74e724dea913a4956426993b82021-12-02T16:06:49ZHighly-sensitive capture of circulating tumor cells using micro-ellipse filters10.1038/s41598-017-00232-62045-2322https://doaj.org/article/a37053e74e724dea913a4956426993b82017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00232-6https://doaj.org/toc/2045-2322Abstract Circulating tumor cells (CTCs) detection, enumeration and characterization with microfluidic chips has critical significance in cancer prognosis offering a non-invasive “liquid biopsy”. Based on physical differences of size and deformability, we explore micro-ellipse filters consisting of microfuidic slits in series gradually narrowed. Slender tunnels sensitively capture tumor cells with slim chance to escape. Tumor cells could reside at capture sites organized by arrays of micro-ellipse microposts enduring less stress. Circular elliptical microstructures produce smooth flow minimally reducing any damage. “Air Suction” could extremely shorten capture. Capture efficiency comes out to be a robust yield of 90% and percentage obeys Gaussian distribution at various stages. With rare number accurately enumerated, micro-Ellipse filters have been tested high efficiently capturing tumor cells in both whole and lysed blood. To clinically validate the device, the microfluidic chip was utilized to identify and capture CTCs from metastatic breast, colon and non-small-cell lung (NSCLC) cancer patients. CTCs were detected positive in all samples with 4 patients having more than 20 CTCs. Those sensitive results are consistent with theoretical expectation. Efficient micro-ellipse filters enable clinical enumeration of metastasis, on-chip anti-cancer drug responses and biological molecular analysis.Hongmei ChenBaoshan CaoBo SunYapeng CaoKe YangYu-Sheng LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongmei Chen Baoshan Cao Bo Sun Yapeng Cao Ke Yang Yu-Sheng Lin Highly-sensitive capture of circulating tumor cells using micro-ellipse filters
description	Abstract Circulating tumor cells (CTCs) detection, enumeration and characterization with microfluidic chips has critical significance in cancer prognosis offering a non-invasive “liquid biopsy”. Based on physical differences of size and deformability, we explore micro-ellipse filters consisting of microfuidic slits in series gradually narrowed. Slender tunnels sensitively capture tumor cells with slim chance to escape. Tumor cells could reside at capture sites organized by arrays of micro-ellipse microposts enduring less stress. Circular elliptical microstructures produce smooth flow minimally reducing any damage. “Air Suction” could extremely shorten capture. Capture efficiency comes out to be a robust yield of 90% and percentage obeys Gaussian distribution at various stages. With rare number accurately enumerated, micro-Ellipse filters have been tested high efficiently capturing tumor cells in both whole and lysed blood. To clinically validate the device, the microfluidic chip was utilized to identify and capture CTCs from metastatic breast, colon and non-small-cell lung (NSCLC) cancer patients. CTCs were detected positive in all samples with 4 patients having more than 20 CTCs. Those sensitive results are consistent with theoretical expectation. Efficient micro-ellipse filters enable clinical enumeration of metastasis, on-chip anti-cancer drug responses and biological molecular analysis.
format	article
author	Hongmei Chen Baoshan Cao Bo Sun Yapeng Cao Ke Yang Yu-Sheng Lin
author_facet	Hongmei Chen Baoshan Cao Bo Sun Yapeng Cao Ke Yang Yu-Sheng Lin
author_sort	Hongmei Chen
title	Highly-sensitive capture of circulating tumor cells using micro-ellipse filters
title_short	Highly-sensitive capture of circulating tumor cells using micro-ellipse filters
title_full	Highly-sensitive capture of circulating tumor cells using micro-ellipse filters
title_fullStr	Highly-sensitive capture of circulating tumor cells using micro-ellipse filters
title_full_unstemmed	Highly-sensitive capture of circulating tumor cells using micro-ellipse filters
title_sort	highly-sensitive capture of circulating tumor cells using micro-ellipse filters
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a37053e74e724dea913a4956426993b8
work_keys_str_mv	AT hongmeichen highlysensitivecaptureofcirculatingtumorcellsusingmicroellipsefilters AT baoshancao highlysensitivecaptureofcirculatingtumorcellsusingmicroellipsefilters AT bosun highlysensitivecaptureofcirculatingtumorcellsusingmicroellipsefilters AT yapengcao highlysensitivecaptureofcirculatingtumorcellsusingmicroellipsefilters AT keyang highlysensitivecaptureofcirculatingtumorcellsusingmicroellipsefilters AT yushenglin highlysensitivecaptureofcirculatingtumorcellsusingmicroellipsefilters
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Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

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