Rift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret

ABSTRACT Rift Valley fever virus (RVFV) is a pathogen of both humans and livestock in Africa and the Middle East. Severe human disease is associated with hepatitis and/or encephalitis. Current pathogenesis studies rely on rodents and nonhuman primates, which have advantages and disadvantages. We eva...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dominique J. Barbeau, Joseph R. Albe, Sham Nambulli, Natasha L. Tilston-Lunel, Amy L. Hartman, Seema S. Lakdawala, Ed Klein, W. Paul Duprex, Anita K. McElroy
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Rift Valley fever virus encephalitis ferret animal model pathogenesis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a37141366b9f4ea5a5bc7702a5492d20
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a37141366b9f4ea5a5bc7702a5492d20
record_format	dspace
spelling	oai:doaj.org-article:a37141366b9f4ea5a5bc7702a5492d202021-11-15T15:30:58ZRift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret10.1128/mSphere.00798-202379-5042https://doaj.org/article/a37141366b9f4ea5a5bc7702a5492d202020-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00798-20https://doaj.org/toc/2379-5042ABSTRACT Rift Valley fever virus (RVFV) is a pathogen of both humans and livestock in Africa and the Middle East. Severe human disease is associated with hepatitis and/or encephalitis. Current pathogenesis studies rely on rodents and nonhuman primates, which have advantages and disadvantages. We evaluated disease progression in Mustela putorius furo (the ferret) following intradermal (i.d.) or intranasal (i.n.) infection. Infected ferrets developed hyperpyrexia, weight loss, lymphopenia, and hypoalbuminemia. Three of four ferrets inoculated intranasally with RVFV developed central nervous system (CNS) disease that manifested as seizure, ataxia, and/or hind limb weakness at 8 to 11 days postinfection (dpi). Animals with clinical CNS disease had transient viral RNAemia, high viral RNA loads in the brain, and histopathological evidence of encephalitis. The ferret model will facilitate our understanding of how RVFV accesses the CNS and has utility for the evaluation of vaccines and/or therapeutics in preventing RVFV CNS disease. IMPORTANCE Animal models of viral disease are very important for understanding how viruses make people sick and for testing out drugs and vaccines to see if they can prevent disease. In this study, we identify the ferret as a model of encephalitis caused by Rift Valley fever virus (RVFV). This novel model will allow researchers to evaluate ways to prevent RVFV encephalitis.Dominique J. BarbeauJoseph R. AlbeSham NambulliNatasha L. Tilston-LunelAmy L. HartmanSeema S. LakdawalaEd KleinW. Paul DuprexAnita K. McElroyAmerican Society for MicrobiologyarticleRift Valley fever virusencephalitisferretanimal modelpathogenesisMicrobiologyQR1-502ENmSphere, Vol 5, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rift Valley fever virus encephalitis ferret animal model pathogenesis Microbiology QR1-502
spellingShingle	Rift Valley fever virus encephalitis ferret animal model pathogenesis Microbiology QR1-502 Dominique J. Barbeau Joseph R. Albe Sham Nambulli Natasha L. Tilston-Lunel Amy L. Hartman Seema S. Lakdawala Ed Klein W. Paul Duprex Anita K. McElroy Rift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret
description	ABSTRACT Rift Valley fever virus (RVFV) is a pathogen of both humans and livestock in Africa and the Middle East. Severe human disease is associated with hepatitis and/or encephalitis. Current pathogenesis studies rely on rodents and nonhuman primates, which have advantages and disadvantages. We evaluated disease progression in Mustela putorius furo (the ferret) following intradermal (i.d.) or intranasal (i.n.) infection. Infected ferrets developed hyperpyrexia, weight loss, lymphopenia, and hypoalbuminemia. Three of four ferrets inoculated intranasally with RVFV developed central nervous system (CNS) disease that manifested as seizure, ataxia, and/or hind limb weakness at 8 to 11 days postinfection (dpi). Animals with clinical CNS disease had transient viral RNAemia, high viral RNA loads in the brain, and histopathological evidence of encephalitis. The ferret model will facilitate our understanding of how RVFV accesses the CNS and has utility for the evaluation of vaccines and/or therapeutics in preventing RVFV CNS disease. IMPORTANCE Animal models of viral disease are very important for understanding how viruses make people sick and for testing out drugs and vaccines to see if they can prevent disease. In this study, we identify the ferret as a model of encephalitis caused by Rift Valley fever virus (RVFV). This novel model will allow researchers to evaluate ways to prevent RVFV encephalitis.
format	article
author	Dominique J. Barbeau Joseph R. Albe Sham Nambulli Natasha L. Tilston-Lunel Amy L. Hartman Seema S. Lakdawala Ed Klein W. Paul Duprex Anita K. McElroy
author_facet	Dominique J. Barbeau Joseph R. Albe Sham Nambulli Natasha L. Tilston-Lunel Amy L. Hartman Seema S. Lakdawala Ed Klein W. Paul Duprex Anita K. McElroy
author_sort	Dominique J. Barbeau
title	Rift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret
title_short	Rift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret
title_full	Rift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret
title_fullStr	Rift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret
title_full_unstemmed	Rift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret
title_sort	rift valley fever virus infection causes acute encephalitis in the ferret
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/a37141366b9f4ea5a5bc7702a5492d20
work_keys_str_mv	AT dominiquejbarbeau riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret AT josephralbe riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret AT shamnambulli riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret AT natashaltilstonlunel riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret AT amylhartman riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret AT seemaslakdawala riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret AT edklein riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret AT wpaulduprex riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret AT anitakmcelroy riftvalleyfevervirusinfectioncausesacuteencephalitisintheferret
_version_	1718427904097910784

Rift Valley Fever Virus Infection Causes Acute Encephalitis in the Ferret

Ejemplares similares