Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes

Endophytic nitrogen-fixing (diazotrophic) bacteria are essential members of the microbiome of switchgrass (Panicum virgatum), considered to be an important commodity crop in bioenergy production. While endophytic diazotrophs are known to provide fixed atmospheric nitrogen to their host plant, there...

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Autores principales:	Sara Gushgari-Doyle, Marcus Schicklberger, Yifan V. Li, Robert Walker, Romy Chakraborty
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	endophyte nitrogen-fixation switchgrass plant growth promoting (PGP) bacteria diazotroph Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a376ff3b00454db5afeb74120648bca0
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spelling	oai:doaj.org-article:a376ff3b00454db5afeb74120648bca02021-11-12T06:15:49ZPlant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes1664-302X10.3389/fmicb.2021.730440https://doaj.org/article/a376ff3b00454db5afeb74120648bca02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.730440/fullhttps://doaj.org/toc/1664-302XEndophytic nitrogen-fixing (diazotrophic) bacteria are essential members of the microbiome of switchgrass (Panicum virgatum), considered to be an important commodity crop in bioenergy production. While endophytic diazotrophs are known to provide fixed atmospheric nitrogen to their host plant, there are many other plant growth-promoting (PGP) capabilities of these organisms to be demonstrated. The diversity of PGP traits across different taxa of switchgrass-colonizing endophytes is understudied, yet critical for understanding endophytic function and improving cultivation methods of important commodity crops. Here, we present the isolation and characterization of three diazotrophic endophytes: Azospirillum agricola R1C, Klebsiella variicola F10Cl, and Raoultella terrigena R1Gly. Strains R1C and F10Cl were isolated from switchgrass and strain R1Gly, while isolated from tobacco, is demonstrated herein to colonize switchgrass. Each strain exhibited highly diverse genomic and phenotypic PGP capabilities. Strain F10Cl and R1Gly demonstrated the highest functional similarity, suggesting that, while endophyte community structure may vary widely based on host species, differences in functional diversity are not a clearly delineated. The results of this study advance our understanding of diazotrophic endophyte diversity, which will allow us to design robust strategies to improve cultivation methods of many economically important commodity crops.Sara Gushgari-DoyleMarcus SchicklbergerYifan V. LiRobert WalkerRomy ChakrabortyFrontiers Media S.A.articleendophytenitrogen-fixationswitchgrassplant growth promoting (PGP) bacteriadiazotrophMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endophyte nitrogen-fixation switchgrass plant growth promoting (PGP) bacteria diazotroph Microbiology QR1-502
spellingShingle	endophyte nitrogen-fixation switchgrass plant growth promoting (PGP) bacteria diazotroph Microbiology QR1-502 Sara Gushgari-Doyle Marcus Schicklberger Yifan V. Li Robert Walker Romy Chakraborty Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes
description	Endophytic nitrogen-fixing (diazotrophic) bacteria are essential members of the microbiome of switchgrass (Panicum virgatum), considered to be an important commodity crop in bioenergy production. While endophytic diazotrophs are known to provide fixed atmospheric nitrogen to their host plant, there are many other plant growth-promoting (PGP) capabilities of these organisms to be demonstrated. The diversity of PGP traits across different taxa of switchgrass-colonizing endophytes is understudied, yet critical for understanding endophytic function and improving cultivation methods of important commodity crops. Here, we present the isolation and characterization of three diazotrophic endophytes: Azospirillum agricola R1C, Klebsiella variicola F10Cl, and Raoultella terrigena R1Gly. Strains R1C and F10Cl were isolated from switchgrass and strain R1Gly, while isolated from tobacco, is demonstrated herein to colonize switchgrass. Each strain exhibited highly diverse genomic and phenotypic PGP capabilities. Strain F10Cl and R1Gly demonstrated the highest functional similarity, suggesting that, while endophyte community structure may vary widely based on host species, differences in functional diversity are not a clearly delineated. The results of this study advance our understanding of diazotrophic endophyte diversity, which will allow us to design robust strategies to improve cultivation methods of many economically important commodity crops.
format	article
author	Sara Gushgari-Doyle Marcus Schicklberger Yifan V. Li Robert Walker Romy Chakraborty
author_facet	Sara Gushgari-Doyle Marcus Schicklberger Yifan V. Li Robert Walker Romy Chakraborty
author_sort	Sara Gushgari-Doyle
title	Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes
title_short	Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes
title_full	Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes
title_fullStr	Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes
title_full_unstemmed	Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes
title_sort	plant growth promotion diversity in switchgrass-colonizing, diazotrophic endophytes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a376ff3b00454db5afeb74120648bca0
work_keys_str_mv	AT saragushgaridoyle plantgrowthpromotiondiversityinswitchgrasscolonizingdiazotrophicendophytes AT marcusschicklberger plantgrowthpromotiondiversityinswitchgrasscolonizingdiazotrophicendophytes AT yifanvli plantgrowthpromotiondiversityinswitchgrasscolonizingdiazotrophicendophytes AT robertwalker plantgrowthpromotiondiversityinswitchgrasscolonizingdiazotrophicendophytes AT romychakraborty plantgrowthpromotiondiversityinswitchgrasscolonizingdiazotrophicendophytes
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Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes

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