Within-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.

Tomato yellow leaf curl virus (TYLCV) is a highly damaging begomovirus native to the Middle East. TYLCV has recently spread worldwide, recombining with other begomoviruses. Recent analysis of mixed infections between TYLCV and Tomato leaf curl Comoros begomovirus (ToLCKMV) has shown that, although n...

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Autores principales:	Cica Urbino, Serafin Gutiérrez, Anna Antolik, Nabila Bouazza, Juliette Doumayrou, Martine Granier, Darren P Martin, Michel Peterschmitt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a38478aa66f445dc914102f0ad42cc6e
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spelling	oai:doaj.org-article:a38478aa66f445dc914102f0ad42cc6e2021-11-18T07:54:48ZWithin-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.1932-620310.1371/journal.pone.0058375https://doaj.org/article/a38478aa66f445dc914102f0ad42cc6e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23472190/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Tomato yellow leaf curl virus (TYLCV) is a highly damaging begomovirus native to the Middle East. TYLCV has recently spread worldwide, recombining with other begomoviruses. Recent analysis of mixed infections between TYLCV and Tomato leaf curl Comoros begomovirus (ToLCKMV) has shown that, although natural selection preserves certain co-evolved intra-genomic interactions, numerous and diverse recombinants are produced at 120 days post-inoculation (dpi), and recombinant populations from different tomato plants are very divergent. Here, we investigate the population dynamics that lead to such patterns in tomato plants co-infected with TYLCV and ToLCKMV either by agro-inoculation or using the natural whitefly vector Bemisia tabaci. We monitored the frequency of parental and recombinant genotypes independently in 35 plants between 18 and 330 dpi and identified 177 recombinants isolated at different times. Recombinants were detected from 18 dpi and their frequency increased over time to reach about 50% at 150 dpi regardless of the inoculation method. The distribution of breakpoints detected on 96 fully sequenced recombinants was consistent with a continuous generation of new recombinants as well as random and deterministic effects in their maintenance. A severe population bottleneck of around 10 genomes was estimated during early systemic infection-a phenomenon that could account partially for the heterogeneity in recombinant patterns observed among plants. The detection of the same recombinant genome in six of the thirteen plants analysed beyond 30 dpi supported the influence of selection on observed recombination patterns. Moreover, a highly virulent recombinant genotype dominating virus populations within one plant has, apparently, the potential to be maintained in the natural population according to its infectivity, within-host accumulation, and transmission efficiency - all of which were similar or intermediate to those of the parent genotypes. Our results anticipate the outcomes of natural encounters between TYLCV and ToLCKMV.Cica UrbinoSerafin GutiérrezAnna AntolikNabila BouazzaJuliette DoumayrouMartine GranierDarren P MartinMichel PeterschmittPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58375 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cica Urbino Serafin Gutiérrez Anna Antolik Nabila Bouazza Juliette Doumayrou Martine Granier Darren P Martin Michel Peterschmitt Within-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.
description	Tomato yellow leaf curl virus (TYLCV) is a highly damaging begomovirus native to the Middle East. TYLCV has recently spread worldwide, recombining with other begomoviruses. Recent analysis of mixed infections between TYLCV and Tomato leaf curl Comoros begomovirus (ToLCKMV) has shown that, although natural selection preserves certain co-evolved intra-genomic interactions, numerous and diverse recombinants are produced at 120 days post-inoculation (dpi), and recombinant populations from different tomato plants are very divergent. Here, we investigate the population dynamics that lead to such patterns in tomato plants co-infected with TYLCV and ToLCKMV either by agro-inoculation or using the natural whitefly vector Bemisia tabaci. We monitored the frequency of parental and recombinant genotypes independently in 35 plants between 18 and 330 dpi and identified 177 recombinants isolated at different times. Recombinants were detected from 18 dpi and their frequency increased over time to reach about 50% at 150 dpi regardless of the inoculation method. The distribution of breakpoints detected on 96 fully sequenced recombinants was consistent with a continuous generation of new recombinants as well as random and deterministic effects in their maintenance. A severe population bottleneck of around 10 genomes was estimated during early systemic infection-a phenomenon that could account partially for the heterogeneity in recombinant patterns observed among plants. The detection of the same recombinant genome in six of the thirteen plants analysed beyond 30 dpi supported the influence of selection on observed recombination patterns. Moreover, a highly virulent recombinant genotype dominating virus populations within one plant has, apparently, the potential to be maintained in the natural population according to its infectivity, within-host accumulation, and transmission efficiency - all of which were similar or intermediate to those of the parent genotypes. Our results anticipate the outcomes of natural encounters between TYLCV and ToLCKMV.
format	article
author	Cica Urbino Serafin Gutiérrez Anna Antolik Nabila Bouazza Juliette Doumayrou Martine Granier Darren P Martin Michel Peterschmitt
author_facet	Cica Urbino Serafin Gutiérrez Anna Antolik Nabila Bouazza Juliette Doumayrou Martine Granier Darren P Martin Michel Peterschmitt
author_sort	Cica Urbino
title	Within-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.
title_short	Within-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.
title_full	Within-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.
title_fullStr	Within-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.
title_full_unstemmed	Within-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.
title_sort	within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a38478aa66f445dc914102f0ad42cc6e
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Within-host dynamics of the emergence of Tomato yellow leaf curl virus recombinants.

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