Resolving the central metabolism of Arabidopsis guard cells

Abstract Photosynthesis and water use efficiency, key factors affecting plant growth, are directly controlled by microscopic and adjustable pores in the leaf—the stomata. The size of the pores is modulated by the guard cells, which rely on molecular mechanisms to sense and respond to environmental c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Semidán Robaina-Estévez, Danilo M. Daloso, Youjun Zhang, Alisdair R. Fernie, Zoran Nikoloski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a399402d1f8b40a280e48e4e675e16ed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a399402d1f8b40a280e48e4e675e16ed
record_format	dspace
spelling	oai:doaj.org-article:a399402d1f8b40a280e48e4e675e16ed2021-12-02T16:06:32ZResolving the central metabolism of Arabidopsis guard cells10.1038/s41598-017-07132-92045-2322https://doaj.org/article/a399402d1f8b40a280e48e4e675e16ed2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07132-9https://doaj.org/toc/2045-2322Abstract Photosynthesis and water use efficiency, key factors affecting plant growth, are directly controlled by microscopic and adjustable pores in the leaf—the stomata. The size of the pores is modulated by the guard cells, which rely on molecular mechanisms to sense and respond to environmental changes. It has been shown that the physiology of mesophyll and guard cells differs substantially. However, the implications of these differences to metabolism at a genome-scale level remain unclear. Here, we used constraint-based modeling to predict the differences in metabolic fluxes between the mesophyll and guard cells of Arabidopsis thaliana by exploring the space of fluxes that are most concordant to cell-type-specific transcript profiles. An independent 13C-labeling experiment using isolated mesophyll and guard cells was conducted and provided support for our predictions about the role of the Calvin-Benson cycle in sucrose synthesis in guard cells. The combination of in silico with in vivo analyses indicated that guard cells have higher anaplerotic CO2 fixation via phosphoenolpyruvate carboxylase, which was demonstrated to be an important source of malate. Beyond highlighting the metabolic differences between mesophyll and guard cells, our findings can be used in future integrated modeling of multi-cellular plant systems and their engineering towards improved growth.Semidán Robaina-EstévezDanilo M. DalosoYoujun ZhangAlisdair R. FernieZoran NikoloskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Semidán Robaina-Estévez Danilo M. Daloso Youjun Zhang Alisdair R. Fernie Zoran Nikoloski Resolving the central metabolism of Arabidopsis guard cells
description	Abstract Photosynthesis and water use efficiency, key factors affecting plant growth, are directly controlled by microscopic and adjustable pores in the leaf—the stomata. The size of the pores is modulated by the guard cells, which rely on molecular mechanisms to sense and respond to environmental changes. It has been shown that the physiology of mesophyll and guard cells differs substantially. However, the implications of these differences to metabolism at a genome-scale level remain unclear. Here, we used constraint-based modeling to predict the differences in metabolic fluxes between the mesophyll and guard cells of Arabidopsis thaliana by exploring the space of fluxes that are most concordant to cell-type-specific transcript profiles. An independent 13C-labeling experiment using isolated mesophyll and guard cells was conducted and provided support for our predictions about the role of the Calvin-Benson cycle in sucrose synthesis in guard cells. The combination of in silico with in vivo analyses indicated that guard cells have higher anaplerotic CO2 fixation via phosphoenolpyruvate carboxylase, which was demonstrated to be an important source of malate. Beyond highlighting the metabolic differences between mesophyll and guard cells, our findings can be used in future integrated modeling of multi-cellular plant systems and their engineering towards improved growth.
format	article
author	Semidán Robaina-Estévez Danilo M. Daloso Youjun Zhang Alisdair R. Fernie Zoran Nikoloski
author_facet	Semidán Robaina-Estévez Danilo M. Daloso Youjun Zhang Alisdair R. Fernie Zoran Nikoloski
author_sort	Semidán Robaina-Estévez
title	Resolving the central metabolism of Arabidopsis guard cells
title_short	Resolving the central metabolism of Arabidopsis guard cells
title_full	Resolving the central metabolism of Arabidopsis guard cells
title_fullStr	Resolving the central metabolism of Arabidopsis guard cells
title_full_unstemmed	Resolving the central metabolism of Arabidopsis guard cells
title_sort	resolving the central metabolism of arabidopsis guard cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a399402d1f8b40a280e48e4e675e16ed
work_keys_str_mv	AT semidanrobainaestevez resolvingthecentralmetabolismofarabidopsisguardcells AT danilomdaloso resolvingthecentralmetabolismofarabidopsisguardcells AT youjunzhang resolvingthecentralmetabolismofarabidopsisguardcells AT alisdairrfernie resolvingthecentralmetabolismofarabidopsisguardcells AT zorannikoloski resolvingthecentralmetabolismofarabidopsisguardcells
_version_	1718384980093042688

Resolving the central metabolism of Arabidopsis guard cells

Ejemplares similares