COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS

The State Research Center for Applied Microbiology and Biotechnology has designed two nutrient media — chocolate agar and PBM-agar to isolate pathogens of purulent bacterial meningitis (PBM). In our previous research using collected microbial strains the media were shown to be highly susceptible and...

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Autores principales:	Ya. V. Podkopaev, L. V. Domotenko, A. N. Kruglov, I. V. Ryabchenko, K. V. Detushev, T. P. Morozova, A. P. Shepelin
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2017
Materias:	chocolate agar haemophilus agar pbm-agar selective supplements culture medium neisseria meningitides streptococcus pneumonia haemophilus influenzae Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a39ba2be1bf040bc875803ec9c2f52bd
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spelling	oai:doaj.org-article:a39ba2be1bf040bc875803ec9c2f52bd2021-11-22T07:09:48ZCOMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS2220-76192313-739810.15789/2220-7619-2016-4-389-394https://doaj.org/article/a39ba2be1bf040bc875803ec9c2f52bd2017-01-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/453https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The State Research Center for Applied Microbiology and Biotechnology has designed two nutrient media — chocolate agar and PBM-agar to isolate pathogens of purulent bacterial meningitis (PBM). In our previous research using collected microbial strains the media were shown to be highly susceptible and to provide the growth of Neisseria meningiti-dis, Streptococcus pneumoniae and Haemophilus influenzae strains, when inoculated with microbial suspensions containing single cells. When isolating Haemophilus influenzae, meningococci, and pneumococci the use of selective additives in both media assures selective isolation of required microorganisms, inhibiting contaminants. The objective of this research was to assess the media in bacteriological tests of clinical samples collected from the upper and lower respiratory tract in humans. The bacteriological plating of throat smear specimens (n = 90) from children and adults at the age of 0 to 66 with disorder of the upper respiratory tract on chocolate agar, PBM-agar and on a control medium in the absence of selective additives resulted in the equal amount of microbial cultures isolated. Of 154 isolated cultures 2, 23 and 9 were attributed to Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae, respectively. The plating of throat smears (n = 10) from healthy people at the age of 30 to 55 on the analyzable and control media in the presence of additives allowed us to selectively isolate Haemophilus influenzae and Streptococcus pneumoniae cultures without a quantitative loss, with contaminants inhibited. By their growth characteristics chocolate agar and PBM-agar were highly competitive with reference media being used in clinical practice for isolating main causative agents of purulent bacterial meningitis.Ya. V. PodkopaevL. V. DomotenkoA. N. KruglovI. V. RyabchenkoK. V. DetushevT. P. MorozovaA. P. ShepelinSankt-Peterburg : NIIÈM imeni Pasteraarticlechocolate agarhaemophilus agarpbm-agarselective supplementsculture mediumneisseria meningitidesstreptococcus pneumoniahaemophilus influenzaeInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 6, Iss 4, Pp 389-394 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	chocolate agar haemophilus agar pbm-agar selective supplements culture medium neisseria meningitides streptococcus pneumonia haemophilus influenzae Infectious and parasitic diseases RC109-216
spellingShingle	chocolate agar haemophilus agar pbm-agar selective supplements culture medium neisseria meningitides streptococcus pneumonia haemophilus influenzae Infectious and parasitic diseases RC109-216 Ya. V. Podkopaev L. V. Domotenko A. N. Kruglov I. V. Ryabchenko K. V. Detushev T. P. Morozova A. P. Shepelin COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS
description	The State Research Center for Applied Microbiology and Biotechnology has designed two nutrient media — chocolate agar and PBM-agar to isolate pathogens of purulent bacterial meningitis (PBM). In our previous research using collected microbial strains the media were shown to be highly susceptible and to provide the growth of Neisseria meningiti-dis, Streptococcus pneumoniae and Haemophilus influenzae strains, when inoculated with microbial suspensions containing single cells. When isolating Haemophilus influenzae, meningococci, and pneumococci the use of selective additives in both media assures selective isolation of required microorganisms, inhibiting contaminants. The objective of this research was to assess the media in bacteriological tests of clinical samples collected from the upper and lower respiratory tract in humans. The bacteriological plating of throat smear specimens (n = 90) from children and adults at the age of 0 to 66 with disorder of the upper respiratory tract on chocolate agar, PBM-agar and on a control medium in the absence of selective additives resulted in the equal amount of microbial cultures isolated. Of 154 isolated cultures 2, 23 and 9 were attributed to Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae, respectively. The plating of throat smears (n = 10) from healthy people at the age of 30 to 55 on the analyzable and control media in the presence of additives allowed us to selectively isolate Haemophilus influenzae and Streptococcus pneumoniae cultures without a quantitative loss, with contaminants inhibited. By their growth characteristics chocolate agar and PBM-agar were highly competitive with reference media being used in clinical practice for isolating main causative agents of purulent bacterial meningitis.
format	article
author	Ya. V. Podkopaev L. V. Domotenko A. N. Kruglov I. V. Ryabchenko K. V. Detushev T. P. Morozova A. P. Shepelin
author_facet	Ya. V. Podkopaev L. V. Domotenko A. N. Kruglov I. V. Ryabchenko K. V. Detushev T. P. Morozova A. P. Shepelin
author_sort	Ya. V. Podkopaev
title	COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS
title_short	COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS
title_full	COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS
title_fullStr	COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS
title_full_unstemmed	COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS
title_sort	comparative evaluation of culture media for pathogen isolation of purulent bacterial meningitis
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2017
url	https://doaj.org/article/a39ba2be1bf040bc875803ec9c2f52bd
work_keys_str_mv	AT yavpodkopaev comparativeevaluationofculturemediaforpathogenisolationofpurulentbacterialmeningitis AT lvdomotenko comparativeevaluationofculturemediaforpathogenisolationofpurulentbacterialmeningitis AT ankruglov comparativeevaluationofculturemediaforpathogenisolationofpurulentbacterialmeningitis AT ivryabchenko comparativeevaluationofculturemediaforpathogenisolationofpurulentbacterialmeningitis AT kvdetushev comparativeevaluationofculturemediaforpathogenisolationofpurulentbacterialmeningitis AT tpmorozova comparativeevaluationofculturemediaforpathogenisolationofpurulentbacterialmeningitis AT apshepelin comparativeevaluationofculturemediaforpathogenisolationofpurulentbacterialmeningitis
_version_	1718418058797645824

COMPARATIVE EVALUATION OF CULTURE MEDIA FOR PATHOGEN ISOLATION OF PURULENT BACTERIAL MENINGITIS

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