Thioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.

<h4>Background</h4>Thioredoxin 80 (Trx80) is an 80 amino acid natural cleavage product of Trx, produced primarily by monocytes. Trx80 induces differentiation of human monocytes into a novel cell type, named Trx80-activated-monocytes (TAMs).<h4>Principal findings</h4>In this i...

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Autores principales:	Ximena Cortes-Bratti, Eugénie Bassères, Fabiola Herrera-Rodriguez, Silvia Botero-Kleiven, Giuseppe Coppotelli, Jens B Andersen, Maria G Masucci, Arne Holmgren, Esteban Chaves-Olarte, Teresa Frisan, Javier Avila-Cariño
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a3a2c7311ab8442e9881fec7e662edd7
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spelling	oai:doaj.org-article:a3a2c7311ab8442e9881fec7e662edd72021-11-18T06:58:32ZThioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.1932-620310.1371/journal.pone.0016960https://doaj.org/article/a3a2c7311ab8442e9881fec7e662edd72011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21365006/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Thioredoxin 80 (Trx80) is an 80 amino acid natural cleavage product of Trx, produced primarily by monocytes. Trx80 induces differentiation of human monocytes into a novel cell type, named Trx80-activated-monocytes (TAMs).<h4>Principal findings</h4>In this investigation we present evidence for a role of TAMs in the control of intracellular bacterial infections. As model pathogens we have chosen Listeria monocytogenes and Brucella abortus which replicate in the cytosol and the endoplasmic reticulum respectively. Our data indicate that TAMs efficiently inhibit intracellular growth of both L. monocytogenes and B. abortus. Further analysis shows that Trx80 activation prevents the escape of GFP-tagged L. monocytogenes into the cytosol, and induces accumulation of the bacteria within the lysosomes. Inhibition of the lysosomal activity by chloroquine treatment resulted in higher replication of bacteria in TAMs compared to that observed in control cells 24 h post-infection, indicating that TAMs kill bacteria by preventing their escape from the endosomal compartments, which progress into a highly degradative phagolysosome.<h4>Significance</h4>Our results show that Trx80 potentiates the bactericidal activities of professional phagocytes, and contributes to the first line of defense against intracellular bacteria.Ximena Cortes-BrattiEugénie BassèresFabiola Herrera-RodriguezSilvia Botero-KleivenGiuseppe CoppotelliJens B AndersenMaria G MasucciArne HolmgrenEsteban Chaves-OlarteTeresa FrisanJavier Avila-CariñoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e16960 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ximena Cortes-Bratti Eugénie Bassères Fabiola Herrera-Rodriguez Silvia Botero-Kleiven Giuseppe Coppotelli Jens B Andersen Maria G Masucci Arne Holmgren Esteban Chaves-Olarte Teresa Frisan Javier Avila-Cariño Thioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.
description	<h4>Background</h4>Thioredoxin 80 (Trx80) is an 80 amino acid natural cleavage product of Trx, produced primarily by monocytes. Trx80 induces differentiation of human monocytes into a novel cell type, named Trx80-activated-monocytes (TAMs).<h4>Principal findings</h4>In this investigation we present evidence for a role of TAMs in the control of intracellular bacterial infections. As model pathogens we have chosen Listeria monocytogenes and Brucella abortus which replicate in the cytosol and the endoplasmic reticulum respectively. Our data indicate that TAMs efficiently inhibit intracellular growth of both L. monocytogenes and B. abortus. Further analysis shows that Trx80 activation prevents the escape of GFP-tagged L. monocytogenes into the cytosol, and induces accumulation of the bacteria within the lysosomes. Inhibition of the lysosomal activity by chloroquine treatment resulted in higher replication of bacteria in TAMs compared to that observed in control cells 24 h post-infection, indicating that TAMs kill bacteria by preventing their escape from the endosomal compartments, which progress into a highly degradative phagolysosome.<h4>Significance</h4>Our results show that Trx80 potentiates the bactericidal activities of professional phagocytes, and contributes to the first line of defense against intracellular bacteria.
format	article
author	Ximena Cortes-Bratti Eugénie Bassères Fabiola Herrera-Rodriguez Silvia Botero-Kleiven Giuseppe Coppotelli Jens B Andersen Maria G Masucci Arne Holmgren Esteban Chaves-Olarte Teresa Frisan Javier Avila-Cariño
author_facet	Ximena Cortes-Bratti Eugénie Bassères Fabiola Herrera-Rodriguez Silvia Botero-Kleiven Giuseppe Coppotelli Jens B Andersen Maria G Masucci Arne Holmgren Esteban Chaves-Olarte Teresa Frisan Javier Avila-Cariño
author_sort	Ximena Cortes-Bratti
title	Thioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.
title_short	Thioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.
title_full	Thioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.
title_fullStr	Thioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.
title_full_unstemmed	Thioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.
title_sort	thioredoxin 80-activated-monocytes (tams) inhibit the replication of intracellular pathogens.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a3a2c7311ab8442e9881fec7e662edd7
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Thioredoxin 80-activated-monocytes (TAMs) inhibit the replication of intracellular pathogens.

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