Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures

Derong Yin,1 Satoshi Komasa,1 Shigeki Yoshimine,1 Tohru Sekino,2 Joji Okazaki11Department of Removable Prosthodontics and Occlusion, Osaka Dental University, Hirakata, Osaka, Japan; 2Advanced Hard Materials, The Institute of Scientific and Industrial Research, Osaka University, Suita, Osaka, JapanPu...

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Autores principales:	Yin D, Komasa S, Yoshimine S, Sekino T, Okazaki J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	surface treatment mussel adhesive protein nano structures osteogenic properties Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a3c0ea9fcf2340218148a22ba5aae595
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spelling	oai:doaj.org-article:a3c0ea9fcf2340218148a22ba5aae5952021-12-02T03:57:12ZEffect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures1178-2013https://doaj.org/article/a3c0ea9fcf2340218148a22ba5aae5952019-05-01T00:00:00Zhttps://www.dovepress.com/effect-of-mussel-adhesive-protein-coating-on-osteogenesis-in-vitro-and-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Derong Yin,1 Satoshi Komasa,1 Shigeki Yoshimine,1 Tohru Sekino,2 Joji Okazaki11Department of Removable Prosthodontics and Occlusion, Osaka Dental University, Hirakata, Osaka, Japan; 2Advanced Hard Materials, The Institute of Scientific and Industrial Research, Osaka University, Suita, Osaka, JapanPurpose: On the basis of reasonable superposition of various surface treatment methods, alkali-treated titanium with nanonetwork structures (TNS) was coated with mussel adhesive protein (MAP) and named TNS-MAP. The aims were to optimize the biological properties of TNS, endue it with new properties, and enhance its utility in clinical dental applications.Methods: TNS disks were coated with MAP and the product surface was characterized. Its osteogenic properties were determined by evaluating its effects on cell adhesion, cell proliferation, the expression of osteogenesis-related genes, and in vivo experiments.Results: The treated materials showed excellent hydrophilicity, good surface roughness, and advantages of both TNS and MAP. TNS-MAP significantly promoted initial cell attachment especially after 15 mins and 30 mins. At every time point, cell adhesion and proliferation, the detection rate of osteogenesis-related markers in the extracellular matrix, and the expression of osteogenesis-related genes were markedly superior on TNS-MAP than the control. The in vivo experiments revealed that TNS-MAP promoted new bone growth around the implants and the bone–implant interface.Conclusion: We verified through in vitro and in vivo experiments that we successfully created an effective TNS-MAP composite implant with excellent biocompatibility and advantages of both its TNS and MAP parent materials. Therefore, the new biocomposite implant material TNS-MAP may potentially serve in practical dentistry and orthopedics.Keywords: biocomposite, bone marrow mesenchymal stem cell, extracellular matrix, nanopore, polydopamineYin DKomasa SYoshimine SSekino TOkazaki JDove Medical Pressarticlesurface treatmentmussel adhesive proteinnano structuresosteogenic propertiesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3831-3843 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	surface treatment mussel adhesive protein nano structures osteogenic properties Medicine (General) R5-920
spellingShingle	surface treatment mussel adhesive protein nano structures osteogenic properties Medicine (General) R5-920 Yin D Komasa S Yoshimine S Sekino T Okazaki J Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures
description	Derong Yin,1 Satoshi Komasa,1 Shigeki Yoshimine,1 Tohru Sekino,2 Joji Okazaki11Department of Removable Prosthodontics and Occlusion, Osaka Dental University, Hirakata, Osaka, Japan; 2Advanced Hard Materials, The Institute of Scientific and Industrial Research, Osaka University, Suita, Osaka, JapanPurpose: On the basis of reasonable superposition of various surface treatment methods, alkali-treated titanium with nanonetwork structures (TNS) was coated with mussel adhesive protein (MAP) and named TNS-MAP. The aims were to optimize the biological properties of TNS, endue it with new properties, and enhance its utility in clinical dental applications.Methods: TNS disks were coated with MAP and the product surface was characterized. Its osteogenic properties were determined by evaluating its effects on cell adhesion, cell proliferation, the expression of osteogenesis-related genes, and in vivo experiments.Results: The treated materials showed excellent hydrophilicity, good surface roughness, and advantages of both TNS and MAP. TNS-MAP significantly promoted initial cell attachment especially after 15 mins and 30 mins. At every time point, cell adhesion and proliferation, the detection rate of osteogenesis-related markers in the extracellular matrix, and the expression of osteogenesis-related genes were markedly superior on TNS-MAP than the control. The in vivo experiments revealed that TNS-MAP promoted new bone growth around the implants and the bone–implant interface.Conclusion: We verified through in vitro and in vivo experiments that we successfully created an effective TNS-MAP composite implant with excellent biocompatibility and advantages of both its TNS and MAP parent materials. Therefore, the new biocomposite implant material TNS-MAP may potentially serve in practical dentistry and orthopedics.Keywords: biocomposite, bone marrow mesenchymal stem cell, extracellular matrix, nanopore, polydopamine
format	article
author	Yin D Komasa S Yoshimine S Sekino T Okazaki J
author_facet	Yin D Komasa S Yoshimine S Sekino T Okazaki J
author_sort	Yin D
title	Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures
title_short	Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures
title_full	Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures
title_fullStr	Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures
title_full_unstemmed	Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures
title_sort	effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/a3c0ea9fcf2340218148a22ba5aae595
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_version_	1718401571009593344

Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures

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