NT-ProBNP levels in saliva and its clinical relevance to heart failure.

<h4>Background</h4>Current blood based diagnostic assays to detect heart failure (HF) have large intra-individual and inter-individual variations which have made it difficult to determine whether the changes in the analyte levels reflect an actual change in disease activity. Human saliva...

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Autores principales:	Jared Yong Yang Foo, Yunxia Wan, Karam Kostner, Alicia Arivalagan, John Atherton, Justin Cooper-White, Goce Dimeski, Chamindie Punyadeera
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a3d318bfe8194195b57f680da0d9f7d8
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spelling	oai:doaj.org-article:a3d318bfe8194195b57f680da0d9f7d82021-11-18T08:10:23ZNT-ProBNP levels in saliva and its clinical relevance to heart failure.1932-620310.1371/journal.pone.0048452https://doaj.org/article/a3d318bfe8194195b57f680da0d9f7d82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23119023/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Current blood based diagnostic assays to detect heart failure (HF) have large intra-individual and inter-individual variations which have made it difficult to determine whether the changes in the analyte levels reflect an actual change in disease activity. Human saliva mirrors the body's health and well being and ∼20% of proteins that are present in blood are also found in saliva. Saliva has numerous advantages over blood as a diagnostic fluid which allows for a non-invasive, simple, and safe sample collection. The aim of our study was to develop an immunoassay to detect NT-proBNP in saliva and to determine if there is a correlation with blood levels.<h4>Methods</h4>Saliva samples were collected from healthy volunteers (n = 40) who had no underlying heart conditions and HF patients (n = 45) at rest. Samples were stored at -80°C until analysis. A customised homogeneous sandwich AlphaLISA((R)) immunoassay was used to quantify NT-proBNP levels in saliva.<h4>Results</h4>Our NT-proBNP immunoassay was validated against a commercial Roche assay on plasma samples collected from HF patients (n = 37) and the correlation was r(2) = 0.78 (p<0.01, y = 1.705× +1910.8). The median salivary NT-proBNP levels in the healthy and HF participants were <16 pg/mL and 76.8 pg/mL, respectively. The salivary NT-proBNP immunoassay showed a clinical sensitivity of 82.2% and specificity of 100%, positive predictive value of 100% and negative predictive value of 83.3%, with an overall diagnostic accuracy of 90.6%.<h4>Conclusion</h4>We have firstly demonstrated that NT-proBNP can be detected in saliva and that the levels were higher in heart failure patients compared with healthy control subjects. Further studies will be needed to demonstrate the clinical relevance of salivary NT-proBNP in unselected, previously undiagnosed populations.Jared Yong Yang FooYunxia WanKaram KostnerAlicia ArivalaganJohn AthertonJustin Cooper-WhiteGoce DimeskiChamindie PunyadeeraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48452 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jared Yong Yang Foo Yunxia Wan Karam Kostner Alicia Arivalagan John Atherton Justin Cooper-White Goce Dimeski Chamindie Punyadeera NT-ProBNP levels in saliva and its clinical relevance to heart failure.
description	<h4>Background</h4>Current blood based diagnostic assays to detect heart failure (HF) have large intra-individual and inter-individual variations which have made it difficult to determine whether the changes in the analyte levels reflect an actual change in disease activity. Human saliva mirrors the body's health and well being and ∼20% of proteins that are present in blood are also found in saliva. Saliva has numerous advantages over blood as a diagnostic fluid which allows for a non-invasive, simple, and safe sample collection. The aim of our study was to develop an immunoassay to detect NT-proBNP in saliva and to determine if there is a correlation with blood levels.<h4>Methods</h4>Saliva samples were collected from healthy volunteers (n = 40) who had no underlying heart conditions and HF patients (n = 45) at rest. Samples were stored at -80°C until analysis. A customised homogeneous sandwich AlphaLISA((R)) immunoassay was used to quantify NT-proBNP levels in saliva.<h4>Results</h4>Our NT-proBNP immunoassay was validated against a commercial Roche assay on plasma samples collected from HF patients (n = 37) and the correlation was r(2) = 0.78 (p<0.01, y = 1.705× +1910.8). The median salivary NT-proBNP levels in the healthy and HF participants were <16 pg/mL and 76.8 pg/mL, respectively. The salivary NT-proBNP immunoassay showed a clinical sensitivity of 82.2% and specificity of 100%, positive predictive value of 100% and negative predictive value of 83.3%, with an overall diagnostic accuracy of 90.6%.<h4>Conclusion</h4>We have firstly demonstrated that NT-proBNP can be detected in saliva and that the levels were higher in heart failure patients compared with healthy control subjects. Further studies will be needed to demonstrate the clinical relevance of salivary NT-proBNP in unselected, previously undiagnosed populations.
format	article
author	Jared Yong Yang Foo Yunxia Wan Karam Kostner Alicia Arivalagan John Atherton Justin Cooper-White Goce Dimeski Chamindie Punyadeera
author_facet	Jared Yong Yang Foo Yunxia Wan Karam Kostner Alicia Arivalagan John Atherton Justin Cooper-White Goce Dimeski Chamindie Punyadeera
author_sort	Jared Yong Yang Foo
title	NT-ProBNP levels in saliva and its clinical relevance to heart failure.
title_short	NT-ProBNP levels in saliva and its clinical relevance to heart failure.
title_full	NT-ProBNP levels in saliva and its clinical relevance to heart failure.
title_fullStr	NT-ProBNP levels in saliva and its clinical relevance to heart failure.
title_full_unstemmed	NT-ProBNP levels in saliva and its clinical relevance to heart failure.
title_sort	nt-probnp levels in saliva and its clinical relevance to heart failure.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a3d318bfe8194195b57f680da0d9f7d8
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NT-ProBNP levels in saliva and its clinical relevance to heart failure.

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