Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia

Background and Aim: Uropathogenic Escherichia coli (UPEC) is commonly involved in urinary tract infections (UTIs), which are generally treated with antibiotics. However, the emergence of multidrug-resistant (MDR) strains of UPEC has made the treatment difficult. There is thus a need to continuously...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Souadkia Sarra, Mbarga Manga Joseph Arsene, Volina Elena Grigorievna, Podoprigora Irina Victorovna, Yashina Natalia Vyacheslavovna, Borechova Marina Nikolaïevna
Formato:	article
Lenguaje:	EN
Publicado:	Veterinary World 2021
Materias:	antibiotics multidrug resistance urinary tract infections uropathogenic escherichia coli Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a3d61f7f513041848a30d9332cb930cf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a3d61f7f513041848a30d9332cb930cf
record_format	dspace
spelling	oai:doaj.org-article:a3d61f7f513041848a30d9332cb930cf2021-11-23T07:38:57ZAntibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia10.14202/IJOH.2021.212-2192455-56732455-8931https://doaj.org/article/a3d61f7f513041848a30d9332cb930cf2021-11-01T00:00:00Zhttps://www.onehealthjournal.org/Vol.7/No.2/9.pdfhttps://doaj.org/toc/2455-5673https://doaj.org/toc/2455-8931Background and Aim: Uropathogenic Escherichia coli (UPEC) is commonly involved in urinary tract infections (UTIs), which are generally treated with antibiotics. However, the emergence of multidrug-resistant (MDR) strains of UPEC has made the treatment difficult. There is thus a need to continuously assess their sensitivity to antibiotics. This study aimed to determine the antibiotic resistance patterns and MDR phenotypes of UPEC strains isolated from children diagnosed with UTIs at the Russian Children's Clinical Hospital in Moscow, Russia. Materials and Methods: Kirby–Bauer's disc diffusion method was used to study the sensitivity to antibiotics of 106 UPEC isolates from urine specimens from children (aged from 9 months to 18 years old) diagnosed with UTIs. The results were interpreted in accordance with the Clinical and Laboratory Standards Institute guidelines and the correlations of variables with the degree to which each antibiotic inhibited the UPEC strains in terms of diameter on the disc were determined using Spearman's rank correlation test. A t-test and principal component analysis were performed to visualize the correlations of the susceptibility of UPEC to antibiotics with the age and sex of the patients. Statistical significance was set at p≤0.05. Results: Among the 106 UPEC strains tested, none (0%) showed resistance to fosfomycin (FO), while 84 (79.2%) were resistant (R) to at least one antibiotic. The highest rates of resistance were observed to amoxicillin (69.8%), ampicillin (62.3%), cefazolin (39.6%), trimethoprim (TR) (37.7%), ceftriaxone (34.9%), and tetracycline (33.0%). Interestingly, 22 (20.8%) strains were R to imipenem. UPEC isolates from males aged 1-6 years were more R to antibiotics than those from the other groups, with the exception of TR, to which UPEC isolates from females aged 13-18 years old were less sensitive (S). The multidrug-resistance (MDR) index ranged between 0.00 and 0.75 and we found that more than a quarter of UPEC (31/106) had an MDR index ≥0.5 and only 22 (20.7%) strains were S to all antibiotics tested (MDR index=0). Finally, Spearman's rank correlation test showed that, with the exception of FO, there were correlations between the inhibition diameters of all other antibiotics. Conclusion: FO is the only antibiotic to which all UPECs were S and may be suggested as the first line of treatment for UPEC. Further research is needed to continue monitoring antibiotic resistance and to investigate the genetic features associated with such resistance observed in this study.Souadkia SarraMbarga Manga Joseph ArseneVolina Elena GrigorievnaPodoprigora Irina VictorovnaYashina Natalia VyacheslavovnaBorechova Marina NikolaïevnaVeterinary Worldarticleantibioticsmultidrug resistanceurinary tract infectionsuropathogenic escherichia coliMedicineRMedicine (General)R5-920ENInternational Journal of One Health, Vol 7, Iss 2, Pp 212-219 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotics multidrug resistance urinary tract infections uropathogenic escherichia coli Medicine R Medicine (General) R5-920
spellingShingle	antibiotics multidrug resistance urinary tract infections uropathogenic escherichia coli Medicine R Medicine (General) R5-920 Souadkia Sarra Mbarga Manga Joseph Arsene Volina Elena Grigorievna Podoprigora Irina Victorovna Yashina Natalia Vyacheslavovna Borechova Marina Nikolaïevna Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia
description	Background and Aim: Uropathogenic Escherichia coli (UPEC) is commonly involved in urinary tract infections (UTIs), which are generally treated with antibiotics. However, the emergence of multidrug-resistant (MDR) strains of UPEC has made the treatment difficult. There is thus a need to continuously assess their sensitivity to antibiotics. This study aimed to determine the antibiotic resistance patterns and MDR phenotypes of UPEC strains isolated from children diagnosed with UTIs at the Russian Children's Clinical Hospital in Moscow, Russia. Materials and Methods: Kirby–Bauer's disc diffusion method was used to study the sensitivity to antibiotics of 106 UPEC isolates from urine specimens from children (aged from 9 months to 18 years old) diagnosed with UTIs. The results were interpreted in accordance with the Clinical and Laboratory Standards Institute guidelines and the correlations of variables with the degree to which each antibiotic inhibited the UPEC strains in terms of diameter on the disc were determined using Spearman's rank correlation test. A t-test and principal component analysis were performed to visualize the correlations of the susceptibility of UPEC to antibiotics with the age and sex of the patients. Statistical significance was set at p≤0.05. Results: Among the 106 UPEC strains tested, none (0%) showed resistance to fosfomycin (FO), while 84 (79.2%) were resistant (R) to at least one antibiotic. The highest rates of resistance were observed to amoxicillin (69.8%), ampicillin (62.3%), cefazolin (39.6%), trimethoprim (TR) (37.7%), ceftriaxone (34.9%), and tetracycline (33.0%). Interestingly, 22 (20.8%) strains were R to imipenem. UPEC isolates from males aged 1-6 years were more R to antibiotics than those from the other groups, with the exception of TR, to which UPEC isolates from females aged 13-18 years old were less sensitive (S). The multidrug-resistance (MDR) index ranged between 0.00 and 0.75 and we found that more than a quarter of UPEC (31/106) had an MDR index ≥0.5 and only 22 (20.7%) strains were S to all antibiotics tested (MDR index=0). Finally, Spearman's rank correlation test showed that, with the exception of FO, there were correlations between the inhibition diameters of all other antibiotics. Conclusion: FO is the only antibiotic to which all UPECs were S and may be suggested as the first line of treatment for UPEC. Further research is needed to continue monitoring antibiotic resistance and to investigate the genetic features associated with such resistance observed in this study.
format	article
author	Souadkia Sarra Mbarga Manga Joseph Arsene Volina Elena Grigorievna Podoprigora Irina Victorovna Yashina Natalia Vyacheslavovna Borechova Marina Nikolaïevna
author_facet	Souadkia Sarra Mbarga Manga Joseph Arsene Volina Elena Grigorievna Podoprigora Irina Victorovna Yashina Natalia Vyacheslavovna Borechova Marina Nikolaïevna
author_sort	Souadkia Sarra
title	Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia
title_short	Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia
title_full	Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia
title_fullStr	Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia
title_full_unstemmed	Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia
title_sort	antibiotic resistance pattern of uropathogenic escherichia coli isolated from children with symptomatic urinary tract infection in moscow, russia
publisher	Veterinary World
publishDate	2021
url	https://doaj.org/article/a3d61f7f513041848a30d9332cb930cf
work_keys_str_mv	AT souadkiasarra antibioticresistancepatternofuropathogenicescherichiacoliisolatedfromchildrenwithsymptomaticurinarytractinfectioninmoscowrussia AT mbargamangajosepharsene antibioticresistancepatternofuropathogenicescherichiacoliisolatedfromchildrenwithsymptomaticurinarytractinfectioninmoscowrussia AT volinaelenagrigorievna antibioticresistancepatternofuropathogenicescherichiacoliisolatedfromchildrenwithsymptomaticurinarytractinfectioninmoscowrussia AT podoprigorairinavictorovna antibioticresistancepatternofuropathogenicescherichiacoliisolatedfromchildrenwithsymptomaticurinarytractinfectioninmoscowrussia AT yashinanataliavyacheslavovna antibioticresistancepatternofuropathogenicescherichiacoliisolatedfromchildrenwithsymptomaticurinarytractinfectioninmoscowrussia AT borechovamarinanikolaievna antibioticresistancepatternofuropathogenicescherichiacoliisolatedfromchildrenwithsymptomaticurinarytractinfectioninmoscowrussia
_version_	1718416843652202496

Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia

Ejemplares similares