Deficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.

The structural maintenance of chromosome (SMC) complex cohesin mediates sister chromatid cohesion established during replication, and damage-induced cohesion formed in response to DSBs post-replication. The translesion synthesis polymerase Polη is required for damage-induced cohesion through a hithe...

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Autores principales:	Pei-Shang Wu, Jan Grosser, Donald P Cameron, Laura Baranello, Lena Ström
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/a3d88a91aed548d38e29c534e4bbc1f5
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spelling	oai:doaj.org-article:a3d88a91aed548d38e29c534e4bbc1f52021-12-02T20:03:20ZDeficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.1553-73901553-740410.1371/journal.pgen.1009763https://doaj.org/article/a3d88a91aed548d38e29c534e4bbc1f52021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009763https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The structural maintenance of chromosome (SMC) complex cohesin mediates sister chromatid cohesion established during replication, and damage-induced cohesion formed in response to DSBs post-replication. The translesion synthesis polymerase Polη is required for damage-induced cohesion through a hitherto unknown mechanism. Since Polη is functionally associated with transcription, and transcription triggers de novo cohesion in Schizosaccharomyces pombe, we hypothesized that transcription facilitates damage-induced cohesion in Saccharomyces cerevisiae. Here, we show dysregulated transcriptional profiles in the Polη null mutant (rad30Δ), where genes involved in chromatin assembly and positive transcription regulation were downregulated. In addition, chromatin association of RNA polymerase II was reduced at promoters and coding regions in rad30Δ compared to WT cells, while occupancy of the H2A.Z variant (Htz1) at promoters was increased in rad30Δ cells. Perturbing histone exchange at promoters inactivated damage-induced cohesion, similarly to deletion of the RAD30 gene. Conversely, altering regulation of transcription elongation suppressed the deficient damage-induced cohesion in rad30Δ cells. Furthermore, transcription inhibition negatively affected formation of damage-induced cohesion. These results indicate that the transcriptional deregulation of the Polη null mutant is connected with its reduced capacity to establish damage-induced cohesion. This also suggests a linkage between regulation of transcription and formation of damage-induced cohesion after replication.Pei-Shang WuJan GrosserDonald P CameronLaura BaranelloLena StrömPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009763 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Pei-Shang Wu Jan Grosser Donald P Cameron Laura Baranello Lena Ström Deficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.
description	The structural maintenance of chromosome (SMC) complex cohesin mediates sister chromatid cohesion established during replication, and damage-induced cohesion formed in response to DSBs post-replication. The translesion synthesis polymerase Polη is required for damage-induced cohesion through a hitherto unknown mechanism. Since Polη is functionally associated with transcription, and transcription triggers de novo cohesion in Schizosaccharomyces pombe, we hypothesized that transcription facilitates damage-induced cohesion in Saccharomyces cerevisiae. Here, we show dysregulated transcriptional profiles in the Polη null mutant (rad30Δ), where genes involved in chromatin assembly and positive transcription regulation were downregulated. In addition, chromatin association of RNA polymerase II was reduced at promoters and coding regions in rad30Δ compared to WT cells, while occupancy of the H2A.Z variant (Htz1) at promoters was increased in rad30Δ cells. Perturbing histone exchange at promoters inactivated damage-induced cohesion, similarly to deletion of the RAD30 gene. Conversely, altering regulation of transcription elongation suppressed the deficient damage-induced cohesion in rad30Δ cells. Furthermore, transcription inhibition negatively affected formation of damage-induced cohesion. These results indicate that the transcriptional deregulation of the Polη null mutant is connected with its reduced capacity to establish damage-induced cohesion. This also suggests a linkage between regulation of transcription and formation of damage-induced cohesion after replication.
format	article
author	Pei-Shang Wu Jan Grosser Donald P Cameron Laura Baranello Lena Ström
author_facet	Pei-Shang Wu Jan Grosser Donald P Cameron Laura Baranello Lena Ström
author_sort	Pei-Shang Wu
title	Deficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.
title_short	Deficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.
title_full	Deficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.
title_fullStr	Deficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.
title_full_unstemmed	Deficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.
title_sort	deficiency of polη in saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a3d88a91aed548d38e29c534e4bbc1f5
work_keys_str_mv	AT peishangwu deficiencyofpolēinsaccharomycescerevisiaerevealstheimpactoftranscriptionondamageinducedcohesion AT jangrosser deficiencyofpolēinsaccharomycescerevisiaerevealstheimpactoftranscriptionondamageinducedcohesion AT donaldpcameron deficiencyofpolēinsaccharomycescerevisiaerevealstheimpactoftranscriptionondamageinducedcohesion AT laurabaranello deficiencyofpolēinsaccharomycescerevisiaerevealstheimpactoftranscriptionondamageinducedcohesion AT lenastrom deficiencyofpolēinsaccharomycescerevisiaerevealstheimpactoftranscriptionondamageinducedcohesion
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Deficiency of Polη in Saccharomyces cerevisiae reveals the impact of transcription on damage-induced cohesion.

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