Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts

Background: Biocompatibility is an essential property for any dental root repair material that may interact with the surrounding periodontal tissues. We hypothesise that the three mineral trioxide aggregate (MTA) restorative brands ProRoot MTA, MTA Flow and Harvard MTA have similar biocompatibility....

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Abdel-Rahman Youssef, Samia Elsherief
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
Materias:
MTA
R
Acceso en línea:https://doaj.org/article/a3dc49e530c948f5a17cc0c66765e220
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:a3dc49e530c948f5a17cc0c66765e220
record_format dspace
spelling oai:doaj.org-article:a3dc49e530c948f5a17cc0c66765e2202021-11-10T04:18:39ZEvaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts1013-905210.1016/j.sdentj.2020.04.009https://doaj.org/article/a3dc49e530c948f5a17cc0c66765e2202021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1013905220301310https://doaj.org/toc/1013-9052Background: Biocompatibility is an essential property for any dental root repair material that may interact with the surrounding periodontal tissues. We hypothesise that the three mineral trioxide aggregate (MTA) restorative brands ProRoot MTA, MTA Flow and Harvard MTA have similar biocompatibility. To test this hypothesis, we compared the cytotoxic effects of these materials on human gingival fibroblast (GF). Methods: MTA cements were prepared, and after completion of setting, they were incubated in Dulbecco's Modified Eagle Medium for 1 day or 4 days to obtain low and high concentrations of MTA elutes respectively. The elutes of MTA supplemented with fetal bovine serum were added to GF and incubated for 3 days at 37 °C and 5% CO2. Untreated cells were used as control. The cell viability was assessed using a 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay at 24, 48 and 72 h. Results: After 24 h, the MTT assay showed that both 1- and 4-day elutes of MTA flow and Harvard MTA reduced cell viability significantly compared to control (P < 0.05). After 48 h, the 1-day elute of ProRoot MTA induced GF proliferation (P = 0.0136) while MTA flow and Harvard MTA were similar to control. After 72 h, the 1-day elute of ProRoot MTA and Harvard MTA induced GF proliferation, while the elute of MTA flow was comparable to control. The 4-day elute of Harvard MTA continued to be cytotoxic to GF after 24 h, 48 h, and 72 h incubation, while the 4-day elute of ProRoot MTA and MTA flow were similar to control. Conclusion: ProRoot MTA and MTA Flow showed comparable biocompatibility. However, the 4-day elute of Harvard MTA was cytotoxic to GF. Further studied are required to assess the cell viability after direct contact with these materials versus eluent in vitro and compare these sealers in the clinical setting.Abdel-Rahman YoussefSamia ElsheriefElsevierarticleCytotoxicityMTT assayMTAGingival fibroblastsMedicineRDentistryRK1-715ENSaudi Dental Journal, Vol 33, Iss 7, Pp 679-686 (2021)
institution DOAJ
collection DOAJ
language EN
topic Cytotoxicity
MTT assay
MTA
Gingival fibroblasts
Medicine
R
Dentistry
RK1-715
spellingShingle Cytotoxicity
MTT assay
MTA
Gingival fibroblasts
Medicine
R
Dentistry
RK1-715
Abdel-Rahman Youssef
Samia Elsherief
Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts
description Background: Biocompatibility is an essential property for any dental root repair material that may interact with the surrounding periodontal tissues. We hypothesise that the three mineral trioxide aggregate (MTA) restorative brands ProRoot MTA, MTA Flow and Harvard MTA have similar biocompatibility. To test this hypothesis, we compared the cytotoxic effects of these materials on human gingival fibroblast (GF). Methods: MTA cements were prepared, and after completion of setting, they were incubated in Dulbecco's Modified Eagle Medium for 1 day or 4 days to obtain low and high concentrations of MTA elutes respectively. The elutes of MTA supplemented with fetal bovine serum were added to GF and incubated for 3 days at 37 °C and 5% CO2. Untreated cells were used as control. The cell viability was assessed using a 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay at 24, 48 and 72 h. Results: After 24 h, the MTT assay showed that both 1- and 4-day elutes of MTA flow and Harvard MTA reduced cell viability significantly compared to control (P < 0.05). After 48 h, the 1-day elute of ProRoot MTA induced GF proliferation (P = 0.0136) while MTA flow and Harvard MTA were similar to control. After 72 h, the 1-day elute of ProRoot MTA and Harvard MTA induced GF proliferation, while the elute of MTA flow was comparable to control. The 4-day elute of Harvard MTA continued to be cytotoxic to GF after 24 h, 48 h, and 72 h incubation, while the 4-day elute of ProRoot MTA and MTA flow were similar to control. Conclusion: ProRoot MTA and MTA Flow showed comparable biocompatibility. However, the 4-day elute of Harvard MTA was cytotoxic to GF. Further studied are required to assess the cell viability after direct contact with these materials versus eluent in vitro and compare these sealers in the clinical setting.
format article
author Abdel-Rahman Youssef
Samia Elsherief
author_facet Abdel-Rahman Youssef
Samia Elsherief
author_sort Abdel-Rahman Youssef
title Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts
title_short Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts
title_full Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts
title_fullStr Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts
title_full_unstemmed Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts
title_sort evaluation of the cytotoxic effects of a new harvard mta compared to mta flow and proroot mta on human gingival fibroblasts
publisher Elsevier
publishDate 2021
url https://doaj.org/article/a3dc49e530c948f5a17cc0c66765e220
work_keys_str_mv AT abdelrahmanyoussef evaluationofthecytotoxiceffectsofanewharvardmtacomparedtomtaflowandprorootmtaonhumangingivalfibroblasts
AT samiaelsherief evaluationofthecytotoxiceffectsofanewharvardmtacomparedtomtaflowandprorootmtaonhumangingivalfibroblasts
_version_ 1718440681261760512