TiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition

Jinghong Yang,1 Hui Zhang,1 Sin Man Chan,1 Ruoqi Li,1 Yu Wu,2 Min Cai,1 Anxun Wang,2 Yan Wang1 1Department of Prosthodontics, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2De...

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Autores principales:	Yang J, Zhang H, Chan SM, Li R, Wu Y, Cai M, Wang A, Wang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	tio2 nanotubes diabetes mellitus reactive oxygen species titanium implant surface modification Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a3dd193e8d6c4ab4bf3c1664220067b0
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spelling	oai:doaj.org-article:a3dd193e8d6c4ab4bf3c1664220067b02021-12-02T10:25:14ZTiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition1178-2013https://doaj.org/article/a3dd193e8d6c4ab4bf3c1664220067b02020-05-01T00:00:00Zhttps://www.dovepress.com/tio2-nanotubes-alleviate-diabetes-induced-osteogenetic-inhibition-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jinghong Yang,1 Hui Zhang,1 Sin Man Chan,1 Ruoqi Li,1 Yu Wu,2 Min Cai,1 Anxun Wang,2 Yan Wang1 1Department of Prosthodontics, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of ChinaCorrespondence: Yan WangHospital of Stomatology, Guanghua School of Stomatology, Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaTel +86-20-83802805Email wangyan9@mail.sysu.edu.cnAnxun WangDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaTel +86-20-87608414Email wang_anxun@aliyun.comBackground: Patients with diabetes mellitus (DM) have a higher failure rate of dental implant treatments. However, whether titanium (Ti) implants with TiO2 nanotubes (TNT) surface can retain their biocompatibility and osteogenetic ability under DM conditions has not been investigated; in addition, their behavior in DM conditions is not well characterized.Materials and Methods: Pure Ti discs were surface treated into the polishing (mechanically polished, MP), sandblasted and acid-etched (SLA), and TNT groups. Scanning electron microscopy was used to examine the surface morphology. The cell adhesion and proliferation ability on different modified Ti surfaces at various glucose concentrations (5.5, 11, 16.5, and 22 mM) was detected by the CCK-8 assay. The osteogenetic ability on different modified Ti surfaces under high-glucose conditions was evaluated by alkaline phosphatase (ALP), osteopontin (OPN) immunofluorescence, Western blot, and Alizarin Red staining in vitro. Detection of cell apoptosis and intracellular reactive oxygen species (ROS) was undertaken both before and after N-acetylcysteine (NAC) treatment to assess the oxidative stress associated with different modified Ti surfaces under high-glucose conditions. An in vivo study was conducted in DM rats with different modified Ti femoral implants. The osteogenetic ability of different modified Ti implants in DM rats was assessed using a micro-CT scan.Results: High-glucose conditions inhibited cell adhesion, proliferation, and osteogenetic ability of different modified Ti surfaces. High-glucose conditions induced higher apoptosis rate and intracellular ROS level on different modified Ti surfaces; these effects were alleviated by NAC. Compared with the SLA surface, the TNT surface alleviated the osteogenetic inhibition induced by high-glucose states by reversing the overproduction of ROS in vitro. In the in vivo experiment, micro-CT scan analysis further confirmed the best osteogenetic ability of TNT surface in rats with DM.Conclusion: TNT surface modification alleviates osteogenetic inhibition induced by DM. It may provide a more favorable Ti implant surface for patients with DM.Keywords: TiO2 nanotubes, diabetes mellitus, reactive oxygen species, titanium implant, surface modificationYang JZhang HChan SMLi RWu YCai MWang AWang YDove Medical Pressarticletio2 nanotubesdiabetes mellitusreactive oxygen speciestitanium implantsurface modificationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3523-3537 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	tio2 nanotubes diabetes mellitus reactive oxygen species titanium implant surface modification Medicine (General) R5-920
spellingShingle	tio2 nanotubes diabetes mellitus reactive oxygen species titanium implant surface modification Medicine (General) R5-920 Yang J Zhang H Chan SM Li R Wu Y Cai M Wang A Wang Y TiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition
description	Jinghong Yang,1 Hui Zhang,1 Sin Man Chan,1 Ruoqi Li,1 Yu Wu,2 Min Cai,1 Anxun Wang,2 Yan Wang1 1Department of Prosthodontics, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of ChinaCorrespondence: Yan WangHospital of Stomatology, Guanghua School of Stomatology, Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaTel +86-20-83802805Email wangyan9@mail.sysu.edu.cnAnxun WangDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaTel +86-20-87608414Email wang_anxun@aliyun.comBackground: Patients with diabetes mellitus (DM) have a higher failure rate of dental implant treatments. However, whether titanium (Ti) implants with TiO2 nanotubes (TNT) surface can retain their biocompatibility and osteogenetic ability under DM conditions has not been investigated; in addition, their behavior in DM conditions is not well characterized.Materials and Methods: Pure Ti discs were surface treated into the polishing (mechanically polished, MP), sandblasted and acid-etched (SLA), and TNT groups. Scanning electron microscopy was used to examine the surface morphology. The cell adhesion and proliferation ability on different modified Ti surfaces at various glucose concentrations (5.5, 11, 16.5, and 22 mM) was detected by the CCK-8 assay. The osteogenetic ability on different modified Ti surfaces under high-glucose conditions was evaluated by alkaline phosphatase (ALP), osteopontin (OPN) immunofluorescence, Western blot, and Alizarin Red staining in vitro. Detection of cell apoptosis and intracellular reactive oxygen species (ROS) was undertaken both before and after N-acetylcysteine (NAC) treatment to assess the oxidative stress associated with different modified Ti surfaces under high-glucose conditions. An in vivo study was conducted in DM rats with different modified Ti femoral implants. The osteogenetic ability of different modified Ti implants in DM rats was assessed using a micro-CT scan.Results: High-glucose conditions inhibited cell adhesion, proliferation, and osteogenetic ability of different modified Ti surfaces. High-glucose conditions induced higher apoptosis rate and intracellular ROS level on different modified Ti surfaces; these effects were alleviated by NAC. Compared with the SLA surface, the TNT surface alleviated the osteogenetic inhibition induced by high-glucose states by reversing the overproduction of ROS in vitro. In the in vivo experiment, micro-CT scan analysis further confirmed the best osteogenetic ability of TNT surface in rats with DM.Conclusion: TNT surface modification alleviates osteogenetic inhibition induced by DM. It may provide a more favorable Ti implant surface for patients with DM.Keywords: TiO2 nanotubes, diabetes mellitus, reactive oxygen species, titanium implant, surface modification
format	article
author	Yang J Zhang H Chan SM Li R Wu Y Cai M Wang A Wang Y
author_facet	Yang J Zhang H Chan SM Li R Wu Y Cai M Wang A Wang Y
author_sort	Yang J
title	TiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition
title_short	TiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition
title_full	TiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition
title_fullStr	TiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition
title_full_unstemmed	TiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition
title_sort	tio2 nanotubes alleviate diabetes-induced osteogenetic inhibition
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/a3dd193e8d6c4ab4bf3c1664220067b0
work_keys_str_mv	AT yangj tio2nanotubesalleviatediabetesinducedosteogeneticinhibition AT zhangh tio2nanotubesalleviatediabetesinducedosteogeneticinhibition AT chansm tio2nanotubesalleviatediabetesinducedosteogeneticinhibition AT lir tio2nanotubesalleviatediabetesinducedosteogeneticinhibition AT wuy tio2nanotubesalleviatediabetesinducedosteogeneticinhibition AT caim tio2nanotubesalleviatediabetesinducedosteogeneticinhibition AT wanga tio2nanotubesalleviatediabetesinducedosteogeneticinhibition AT wangy tio2nanotubesalleviatediabetesinducedosteogeneticinhibition
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TiO2 Nanotubes Alleviate Diabetes-Induced Osteogenetic Inhibition

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