IVIG regulates the survival of human but not mouse neutrophils

Abstract Intravenous immunoglobulin (IVIG) are purified IgG preparations made from the pooled plasma from thousands of healthy donors and are being tested in preclinical mouse models. Inherent challenges, however, are the pluripotency of IVIG and its xenogeneicity in animals. IVIG can alter the viab...

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Autores principales:	Christoph Schneider, Simone Wicki, Stefanie Graeter, Tankica M. Timcheva, Christian W. Keller, Isaak Quast, Danila Leontyev, Iglika K. Djoumerska-Alexieva, Fabian Käsermann, Stephan M. Jakob, Petya A. Dimitrova, Donald R. Branch, Richard D. Cummings, Jan D. Lünemann, Thomas Kaufmann, Hans-Uwe Simon, Stephan von Gunten
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a3ebe2f0459b4ca9a78b2c3ea77bd2eb
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spelling	oai:doaj.org-article:a3ebe2f0459b4ca9a78b2c3ea77bd2eb2021-12-02T16:06:55ZIVIG regulates the survival of human but not mouse neutrophils10.1038/s41598-017-01404-02045-2322https://doaj.org/article/a3ebe2f0459b4ca9a78b2c3ea77bd2eb2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01404-0https://doaj.org/toc/2045-2322Abstract Intravenous immunoglobulin (IVIG) are purified IgG preparations made from the pooled plasma from thousands of healthy donors and are being tested in preclinical mouse models. Inherent challenges, however, are the pluripotency of IVIG and its xenogeneicity in animals. IVIG can alter the viability of human neutrophils via agonistic antibodies to Fas and Siglec-9. In this study, we compared the effects of IVIG on human and mouse neutrophils using different death assays. Different commercial IVIG preparations similarly induced cytokine-dependent death in human neutrophils, whereas they had no effects on the survival of either peripheral blood or bone marrow neutrophils from C57BL/6 or BALB/c mice. F(ab’)2 but not Fc fragments of IVIG induced death of human neutrophils, whereas neither of these IVIG fragments, nor agonistic monoclonal antibodies to human Fas or Siglec-9 affected the viability of mouse neutrophils. Pooled mouse IgG, which exhibited a different immunoprofile compared to IVIG, also had no effect on mouse cells. Together, these observations demonstrate that effects of IVIG on neutrophil survival are not adequately reflected in current mouse models, despite the key role of these cells in human inflammatory and autoimmune diseases.Christoph SchneiderSimone WickiStefanie GraeterTankica M. TimchevaChristian W. KellerIsaak QuastDanila LeontyevIglika K. Djoumerska-AlexievaFabian KäsermannStephan M. JakobPetya A. DimitrovaDonald R. BranchRichard D. CummingsJan D. LünemannThomas KaufmannHans-Uwe SimonStephan von GuntenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christoph Schneider Simone Wicki Stefanie Graeter Tankica M. Timcheva Christian W. Keller Isaak Quast Danila Leontyev Iglika K. Djoumerska-Alexieva Fabian Käsermann Stephan M. Jakob Petya A. Dimitrova Donald R. Branch Richard D. Cummings Jan D. Lünemann Thomas Kaufmann Hans-Uwe Simon Stephan von Gunten IVIG regulates the survival of human but not mouse neutrophils
description	Abstract Intravenous immunoglobulin (IVIG) are purified IgG preparations made from the pooled plasma from thousands of healthy donors and are being tested in preclinical mouse models. Inherent challenges, however, are the pluripotency of IVIG and its xenogeneicity in animals. IVIG can alter the viability of human neutrophils via agonistic antibodies to Fas and Siglec-9. In this study, we compared the effects of IVIG on human and mouse neutrophils using different death assays. Different commercial IVIG preparations similarly induced cytokine-dependent death in human neutrophils, whereas they had no effects on the survival of either peripheral blood or bone marrow neutrophils from C57BL/6 or BALB/c mice. F(ab’)2 but not Fc fragments of IVIG induced death of human neutrophils, whereas neither of these IVIG fragments, nor agonistic monoclonal antibodies to human Fas or Siglec-9 affected the viability of mouse neutrophils. Pooled mouse IgG, which exhibited a different immunoprofile compared to IVIG, also had no effect on mouse cells. Together, these observations demonstrate that effects of IVIG on neutrophil survival are not adequately reflected in current mouse models, despite the key role of these cells in human inflammatory and autoimmune diseases.
format	article
author	Christoph Schneider Simone Wicki Stefanie Graeter Tankica M. Timcheva Christian W. Keller Isaak Quast Danila Leontyev Iglika K. Djoumerska-Alexieva Fabian Käsermann Stephan M. Jakob Petya A. Dimitrova Donald R. Branch Richard D. Cummings Jan D. Lünemann Thomas Kaufmann Hans-Uwe Simon Stephan von Gunten
author_facet	Christoph Schneider Simone Wicki Stefanie Graeter Tankica M. Timcheva Christian W. Keller Isaak Quast Danila Leontyev Iglika K. Djoumerska-Alexieva Fabian Käsermann Stephan M. Jakob Petya A. Dimitrova Donald R. Branch Richard D. Cummings Jan D. Lünemann Thomas Kaufmann Hans-Uwe Simon Stephan von Gunten
author_sort	Christoph Schneider
title	IVIG regulates the survival of human but not mouse neutrophils
title_short	IVIG regulates the survival of human but not mouse neutrophils
title_full	IVIG regulates the survival of human but not mouse neutrophils
title_fullStr	IVIG regulates the survival of human but not mouse neutrophils
title_full_unstemmed	IVIG regulates the survival of human but not mouse neutrophils
title_sort	ivig regulates the survival of human but not mouse neutrophils
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a3ebe2f0459b4ca9a78b2c3ea77bd2eb
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IVIG regulates the survival of human but not mouse neutrophils

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