Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips

Abstract Filamentous fungi grow exclusively at their tips, where many growth-related fungal processes, such as enzyme secretion and invasion into host cells, take place. Hyphal tips are also a site of active metabolism. Understanding metabolic dynamics within the tip region is therefore important fo...

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Autores principales:	Mitsuru Yasuda, Norio Takeshita, Shinsuke Shigeto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a3f2f67a0b4142148505c314af5605fb
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spelling	oai:doaj.org-article:a3f2f67a0b4142148505c314af5605fb2021-12-02T14:01:19ZDeuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips10.1038/s41598-020-80270-92045-2322https://doaj.org/article/a3f2f67a0b4142148505c314af5605fb2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80270-9https://doaj.org/toc/2045-2322Abstract Filamentous fungi grow exclusively at their tips, where many growth-related fungal processes, such as enzyme secretion and invasion into host cells, take place. Hyphal tips are also a site of active metabolism. Understanding metabolic dynamics within the tip region is therefore important for biotechnology and medicine as well as for microbiology and ecology. However, methods that can track metabolic dynamics with sufficient spatial resolution and in a nondestructive manner are highly limited. Here we present time-lapse Raman imaging using a deuterium (D) tracer to study spatiotemporally varying metabolic activity within the hyphal tip of Aspergillus nidulans. By analyzing the carbon–deuterium (C–D) stretching Raman band with spectral deconvolution, we visualize glucose accumulation along the inner edge of the hyphal tip and synthesis of new proteins from the taken-up D-labeled glucose specifically at the central part of the apical region. Our results show that deuterium-labeled Raman imaging offers a broadly applicable platform for the study of metabolic dynamics in filamentous fungi and other relevant microorganisms in vivo.Mitsuru YasudaNorio TakeshitaShinsuke ShigetoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitsuru Yasuda Norio Takeshita Shinsuke Shigeto Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips
description	Abstract Filamentous fungi grow exclusively at their tips, where many growth-related fungal processes, such as enzyme secretion and invasion into host cells, take place. Hyphal tips are also a site of active metabolism. Understanding metabolic dynamics within the tip region is therefore important for biotechnology and medicine as well as for microbiology and ecology. However, methods that can track metabolic dynamics with sufficient spatial resolution and in a nondestructive manner are highly limited. Here we present time-lapse Raman imaging using a deuterium (D) tracer to study spatiotemporally varying metabolic activity within the hyphal tip of Aspergillus nidulans. By analyzing the carbon–deuterium (C–D) stretching Raman band with spectral deconvolution, we visualize glucose accumulation along the inner edge of the hyphal tip and synthesis of new proteins from the taken-up D-labeled glucose specifically at the central part of the apical region. Our results show that deuterium-labeled Raman imaging offers a broadly applicable platform for the study of metabolic dynamics in filamentous fungi and other relevant microorganisms in vivo.
format	article
author	Mitsuru Yasuda Norio Takeshita Shinsuke Shigeto
author_facet	Mitsuru Yasuda Norio Takeshita Shinsuke Shigeto
author_sort	Mitsuru Yasuda
title	Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips
title_short	Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips
title_full	Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips
title_fullStr	Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips
title_full_unstemmed	Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips
title_sort	deuterium-labeled raman tracking of glucose accumulation and protein metabolic dynamics in aspergillus nidulans hyphal tips
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a3f2f67a0b4142148505c314af5605fb
work_keys_str_mv	AT mitsuruyasuda deuteriumlabeledramantrackingofglucoseaccumulationandproteinmetabolicdynamicsinaspergillusnidulanshyphaltips AT noriotakeshita deuteriumlabeledramantrackingofglucoseaccumulationandproteinmetabolicdynamicsinaspergillusnidulanshyphaltips AT shinsukeshigeto deuteriumlabeledramantrackingofglucoseaccumulationandproteinmetabolicdynamicsinaspergillusnidulanshyphaltips
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Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips

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