Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei

Abstract Trichoderma reesei is an ascomycete fungus known for its capability to secrete high amounts of extracellular cellulose- and hemicellulose-degrading enzymes. These enzymes are utilized in the production of second-generation biofuels and T. reesei is a well-established host for their producti...

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Autores principales:	Sami Havukainen, Jonai Pujol-Giménez, Mari Valkonen, Ann Westerholm-Parvinen, Matthias A. Hediger, Christopher P. Landowski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a3f3643ea6a143de8f982d5be4fdd2fb
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spelling	oai:doaj.org-article:a3f3643ea6a143de8f982d5be4fdd2fb2021-12-02T16:17:28ZElectrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei10.1038/s41598-021-93552-72045-2322https://doaj.org/article/a3f3643ea6a143de8f982d5be4fdd2fb2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93552-7https://doaj.org/toc/2045-2322Abstract Trichoderma reesei is an ascomycete fungus known for its capability to secrete high amounts of extracellular cellulose- and hemicellulose-degrading enzymes. These enzymes are utilized in the production of second-generation biofuels and T. reesei is a well-established host for their production. Although this species has gained considerable interest in the scientific literature, the sugar transportome of T. reesei remains poorly characterized. Better understanding of the proteins involved in the transport of different sugars could be utilized for engineering better enzyme production strains. In this study we aimed to shed light on this matter by characterizing multiple T. reesei transporters capable of transporting various types of sugars. We used phylogenetics to select transporters for expression in Xenopus laevis oocytes to screen for transport activities. Of the 18 tested transporters, 8 were found to be functional in oocytes. 10 transporters in total were investigated in oocytes and in yeast, and for 3 of them no transport function had been described in literature. This comprehensive analysis provides a large body of new knowledge about T. reesei sugar transporters, and further establishes X. laevis oocytes as a valuable tool for studying fungal sugar transporters.Sami HavukainenJonai Pujol-GiménezMari ValkonenAnn Westerholm-ParvinenMatthias A. HedigerChristopher P. LandowskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sami Havukainen Jonai Pujol-Giménez Mari Valkonen Ann Westerholm-Parvinen Matthias A. Hediger Christopher P. Landowski Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei
description	Abstract Trichoderma reesei is an ascomycete fungus known for its capability to secrete high amounts of extracellular cellulose- and hemicellulose-degrading enzymes. These enzymes are utilized in the production of second-generation biofuels and T. reesei is a well-established host for their production. Although this species has gained considerable interest in the scientific literature, the sugar transportome of T. reesei remains poorly characterized. Better understanding of the proteins involved in the transport of different sugars could be utilized for engineering better enzyme production strains. In this study we aimed to shed light on this matter by characterizing multiple T. reesei transporters capable of transporting various types of sugars. We used phylogenetics to select transporters for expression in Xenopus laevis oocytes to screen for transport activities. Of the 18 tested transporters, 8 were found to be functional in oocytes. 10 transporters in total were investigated in oocytes and in yeast, and for 3 of them no transport function had been described in literature. This comprehensive analysis provides a large body of new knowledge about T. reesei sugar transporters, and further establishes X. laevis oocytes as a valuable tool for studying fungal sugar transporters.
format	article
author	Sami Havukainen Jonai Pujol-Giménez Mari Valkonen Ann Westerholm-Parvinen Matthias A. Hediger Christopher P. Landowski
author_facet	Sami Havukainen Jonai Pujol-Giménez Mari Valkonen Ann Westerholm-Parvinen Matthias A. Hediger Christopher P. Landowski
author_sort	Sami Havukainen
title	Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei
title_short	Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei
title_full	Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei
title_fullStr	Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei
title_full_unstemmed	Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei
title_sort	electrophysiological characterization of a diverse group of sugar transporters from trichoderma reesei
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a3f3643ea6a143de8f982d5be4fdd2fb
work_keys_str_mv	AT samihavukainen electrophysiologicalcharacterizationofadiversegroupofsugartransportersfromtrichodermareesei AT jonaipujolgimenez electrophysiologicalcharacterizationofadiversegroupofsugartransportersfromtrichodermareesei AT marivalkonen electrophysiologicalcharacterizationofadiversegroupofsugartransportersfromtrichodermareesei AT annwesterholmparvinen electrophysiologicalcharacterizationofadiversegroupofsugartransportersfromtrichodermareesei AT matthiasahediger electrophysiologicalcharacterizationofadiversegroupofsugartransportersfromtrichodermareesei AT christopherplandowski electrophysiologicalcharacterizationofadiversegroupofsugartransportersfromtrichodermareesei
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Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei

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