Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.

<h4>Background</h4>Periprosthetic bacterial infections represent one of the most challenging orthopaedic complications that often require implant removal and surgical debridement and carry high social and economical costs. Diabetes is one of the most relevant risk factors of implant-rela...

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Autores principales:	Arianna B Lovati, Lorenzo Drago, Lorenzo Monti, Elena De Vecchi, Sara Previdi, Giuseppe Banfi, Carlo L Romanò
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a3f7b17614b94ad491e3e83ada86d2e2
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spelling	oai:doaj.org-article:a3f7b17614b94ad491e3e83ada86d2e22021-11-18T07:40:54ZDiabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.1932-620310.1371/journal.pone.0067628https://doaj.org/article/a3f7b17614b94ad491e3e83ada86d2e22013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23818985/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Periprosthetic bacterial infections represent one of the most challenging orthopaedic complications that often require implant removal and surgical debridement and carry high social and economical costs. Diabetes is one of the most relevant risk factors of implant-related infection and its clinical occurrence is growing worldwide. The aim of the present study was to test a model of implant-related infection in the diabetic mouse, with a view to allow further investigation on the relative efficacy of prevention and treatment options in diabetic and non-diabetic individuals.<h4>Methodology</h4>A cohort of diabetic NOD/ShiLtJ mice was compared with non-diabetic CD1 mice as an in vivo model of S. aureus orthopaedic infection of bone and soft tissues after femur intramedullary pin implantation. We tested control and infected groups with 1×10(3) colony-forming units of S. aureus ATCC 25923 strain injected in the implant site. At 4 weeks post-inoculation, host response to infection, microbial biofilm formation, and bone damage were assessed by traditional diagnostic parameters (bacterial culture, C-reactive protein and white blood cell count), histological analysis and imaging techniques (micro computed tomography and scanning electron microscopy).<h4>Results</h4>Unlike the controls and the CD1 mice, all the diabetic mice challenged with a single inoculum of S. aureus displayed severe osteomyelitic changes around the implant.<h4>Conclusions</h4>Our findings demonstrate for the first time that the diabetic mouse can be successfully used in a model of orthopaedic implant-related infection. Furthermore, the same bacteria inoculum induced periprosthetic infection in all the diabetic mice but not in the controls. This animal model of implant-related infection in diabetes may be a useful tool to test in vivo treatments in diabetic and non-diabetic individuals.Arianna B LovatiLorenzo DragoLorenzo MontiElena De VecchiSara PrevidiGiuseppe BanfiCarlo L RomanòPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e67628 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arianna B Lovati Lorenzo Drago Lorenzo Monti Elena De Vecchi Sara Previdi Giuseppe Banfi Carlo L Romanò Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.
description	<h4>Background</h4>Periprosthetic bacterial infections represent one of the most challenging orthopaedic complications that often require implant removal and surgical debridement and carry high social and economical costs. Diabetes is one of the most relevant risk factors of implant-related infection and its clinical occurrence is growing worldwide. The aim of the present study was to test a model of implant-related infection in the diabetic mouse, with a view to allow further investigation on the relative efficacy of prevention and treatment options in diabetic and non-diabetic individuals.<h4>Methodology</h4>A cohort of diabetic NOD/ShiLtJ mice was compared with non-diabetic CD1 mice as an in vivo model of S. aureus orthopaedic infection of bone and soft tissues after femur intramedullary pin implantation. We tested control and infected groups with 1×10(3) colony-forming units of S. aureus ATCC 25923 strain injected in the implant site. At 4 weeks post-inoculation, host response to infection, microbial biofilm formation, and bone damage were assessed by traditional diagnostic parameters (bacterial culture, C-reactive protein and white blood cell count), histological analysis and imaging techniques (micro computed tomography and scanning electron microscopy).<h4>Results</h4>Unlike the controls and the CD1 mice, all the diabetic mice challenged with a single inoculum of S. aureus displayed severe osteomyelitic changes around the implant.<h4>Conclusions</h4>Our findings demonstrate for the first time that the diabetic mouse can be successfully used in a model of orthopaedic implant-related infection. Furthermore, the same bacteria inoculum induced periprosthetic infection in all the diabetic mice but not in the controls. This animal model of implant-related infection in diabetes may be a useful tool to test in vivo treatments in diabetic and non-diabetic individuals.
format	article
author	Arianna B Lovati Lorenzo Drago Lorenzo Monti Elena De Vecchi Sara Previdi Giuseppe Banfi Carlo L Romanò
author_facet	Arianna B Lovati Lorenzo Drago Lorenzo Monti Elena De Vecchi Sara Previdi Giuseppe Banfi Carlo L Romanò
author_sort	Arianna B Lovati
title	Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.
title_short	Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.
title_full	Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.
title_fullStr	Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.
title_full_unstemmed	Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.
title_sort	diabetic mouse model of orthopaedic implant-related staphylococcus aureus infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a3f7b17614b94ad491e3e83ada86d2e2
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Diabetic mouse model of orthopaedic implant-related Staphylococcus aureus infection.

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