Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics

Optical aberrations in Structured Illumination Microscopy (SIM) can lead to loss of resolution and artifacts making it unsuitable for thick samples. Here the authors combine Adaptive Optics and SIM (AO-3DSIM) to improve the 3D resolution and reduce artifacts, performing 3D-SIM in C.elegans.

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Autores principales:	Ruizhe Lin, Edward T. Kipreos, Jie Zhu, Chang Hyun Khang, Peter Kner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a3f9d7e799a648b8867781c8ab1af286
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spelling	oai:doaj.org-article:a3f9d7e799a648b8867781c8ab1af2862021-12-02T14:42:18ZSubcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics10.1038/s41467-021-23449-62041-1723https://doaj.org/article/a3f9d7e799a648b8867781c8ab1af2862021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23449-6https://doaj.org/toc/2041-1723Optical aberrations in Structured Illumination Microscopy (SIM) can lead to loss of resolution and artifacts making it unsuitable for thick samples. Here the authors combine Adaptive Optics and SIM (AO-3DSIM) to improve the 3D resolution and reduce artifacts, performing 3D-SIM in C.elegans.Ruizhe LinEdward T. KipreosJie ZhuChang Hyun KhangPeter KnerNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ruizhe Lin Edward T. Kipreos Jie Zhu Chang Hyun Khang Peter Kner Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
description	Optical aberrations in Structured Illumination Microscopy (SIM) can lead to loss of resolution and artifacts making it unsuitable for thick samples. Here the authors combine Adaptive Optics and SIM (AO-3DSIM) to improve the 3D resolution and reduce artifacts, performing 3D-SIM in C.elegans.
format	article
author	Ruizhe Lin Edward T. Kipreos Jie Zhu Chang Hyun Khang Peter Kner
author_facet	Ruizhe Lin Edward T. Kipreos Jie Zhu Chang Hyun Khang Peter Kner
author_sort	Ruizhe Lin
title	Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_short	Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_full	Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_fullStr	Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_full_unstemmed	Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_sort	subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a3f9d7e799a648b8867781c8ab1af286
work_keys_str_mv	AT ruizhelin subcellularthreedimensionalimagingdeepthroughmulticellularthicksamplesbystructuredilluminationmicroscopyandadaptiveoptics AT edwardtkipreos subcellularthreedimensionalimagingdeepthroughmulticellularthicksamplesbystructuredilluminationmicroscopyandadaptiveoptics AT jiezhu subcellularthreedimensionalimagingdeepthroughmulticellularthicksamplesbystructuredilluminationmicroscopyandadaptiveoptics AT changhyunkhang subcellularthreedimensionalimagingdeepthroughmulticellularthicksamplesbystructuredilluminationmicroscopyandadaptiveoptics AT peterkner subcellularthreedimensionalimagingdeepthroughmulticellularthicksamplesbystructuredilluminationmicroscopyandadaptiveoptics
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Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics

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