Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics

Optical aberrations in Structured Illumination Microscopy (SIM) can lead to loss of resolution and artifacts making it unsuitable for thick samples. Here the authors combine Adaptive Optics and SIM (AO-3DSIM) to improve the 3D resolution and reduce artifacts, performing 3D-SIM in C.elegans.

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Autores principales: Ruizhe Lin, Edward T. Kipreos, Jie Zhu, Chang Hyun Khang, Peter Kner
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/a3f9d7e799a648b8867781c8ab1af286
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