Comprehensive Metabolomics Study in Children With Graves’ Disease

ObjectiveGraves’ disease (GD) related hyperthyroidism (HT) has profound effects on metabolic activity and metabolism of macromolecules affecting energy homeostasis. In this study, we aimed to get a comprehensive understanding of the metabolic changes and their clinical relevance in GD children.Metho...

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Autores principales:	Qin Xia, Weifeng Qian, Linqi Chen, Xiuli Chen, Rongrong Xie, Dandan Zhang, Haiying Wu, Hui Sun, Fengyun Wang, Jingjing Liu, Ting Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Graves’ disease children serum metabolomics untargeted metabolomics metabolic pathway Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/a3fa8dc095af40b991229e5f54e55f46
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spelling	oai:doaj.org-article:a3fa8dc095af40b991229e5f54e55f462021-11-16T06:43:12ZComprehensive Metabolomics Study in Children With Graves’ Disease1664-239210.3389/fendo.2021.752496https://doaj.org/article/a3fa8dc095af40b991229e5f54e55f462021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.752496/fullhttps://doaj.org/toc/1664-2392ObjectiveGraves’ disease (GD) related hyperthyroidism (HT) has profound effects on metabolic activity and metabolism of macromolecules affecting energy homeostasis. In this study, we aimed to get a comprehensive understanding of the metabolic changes and their clinical relevance in GD children.MethodsWe investigated serum substances from 30 newly diagnosed GD children and 30 age- and gender-matched healthy controls. We explored the metabolomics using ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) analysis, and then analyzed the metabolomic data via multivariate statistical analysis.ResultsBy untargeted metabolomic analysis, a total of 730 metabolites were identified in all participants, among which 48 differential metabolites between GD and control groups were filtered out, including amino acids, dipeptides, lipids, purines, etc. Among these metabolites, 33 were detected with higher levels, while 15 with lower levels in GD group compared to controls. Pathway analysis showed that HT had a significant impact on aminoacyl-transfer ribonucleic acid (tRNA) biosynthesis, several amino acids metabolism, purine metabolism, and pyrimidine metabolism.ConclusionIn this study, via untargeted metabolomics analysis, significant variations of serum metabolomic patterns were detected in GD children.Qin XiaWeifeng QianLinqi ChenXiuli ChenRongrong XieDandan ZhangHaiying WuHui SunFengyun WangJingjing LiuTing ChenFrontiers Media S.A.articleGraves’ diseasechildrenserum metabolomicsuntargeted metabolomicsmetabolic pathwayDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Graves’ disease children serum metabolomics untargeted metabolomics metabolic pathway Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	Graves’ disease children serum metabolomics untargeted metabolomics metabolic pathway Diseases of the endocrine glands. Clinical endocrinology RC648-665 Qin Xia Weifeng Qian Linqi Chen Xiuli Chen Rongrong Xie Dandan Zhang Haiying Wu Hui Sun Fengyun Wang Jingjing Liu Ting Chen Comprehensive Metabolomics Study in Children With Graves’ Disease
description	ObjectiveGraves’ disease (GD) related hyperthyroidism (HT) has profound effects on metabolic activity and metabolism of macromolecules affecting energy homeostasis. In this study, we aimed to get a comprehensive understanding of the metabolic changes and their clinical relevance in GD children.MethodsWe investigated serum substances from 30 newly diagnosed GD children and 30 age- and gender-matched healthy controls. We explored the metabolomics using ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) analysis, and then analyzed the metabolomic data via multivariate statistical analysis.ResultsBy untargeted metabolomic analysis, a total of 730 metabolites were identified in all participants, among which 48 differential metabolites between GD and control groups were filtered out, including amino acids, dipeptides, lipids, purines, etc. Among these metabolites, 33 were detected with higher levels, while 15 with lower levels in GD group compared to controls. Pathway analysis showed that HT had a significant impact on aminoacyl-transfer ribonucleic acid (tRNA) biosynthesis, several amino acids metabolism, purine metabolism, and pyrimidine metabolism.ConclusionIn this study, via untargeted metabolomics analysis, significant variations of serum metabolomic patterns were detected in GD children.
format	article
author	Qin Xia Weifeng Qian Linqi Chen Xiuli Chen Rongrong Xie Dandan Zhang Haiying Wu Hui Sun Fengyun Wang Jingjing Liu Ting Chen
author_facet	Qin Xia Weifeng Qian Linqi Chen Xiuli Chen Rongrong Xie Dandan Zhang Haiying Wu Hui Sun Fengyun Wang Jingjing Liu Ting Chen
author_sort	Qin Xia
title	Comprehensive Metabolomics Study in Children With Graves’ Disease
title_short	Comprehensive Metabolomics Study in Children With Graves’ Disease
title_full	Comprehensive Metabolomics Study in Children With Graves’ Disease
title_fullStr	Comprehensive Metabolomics Study in Children With Graves’ Disease
title_full_unstemmed	Comprehensive Metabolomics Study in Children With Graves’ Disease
title_sort	comprehensive metabolomics study in children with graves’ disease
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a3fa8dc095af40b991229e5f54e55f46
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Comprehensive Metabolomics Study in Children With Graves’ Disease

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