Chronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes

ABSTRACT Excessive weight and obesity are associated with the development of diabetes mellitus type 2 (DMII) in humans. They also pose high risks of Staphylococcus aureus colonization and overt infections. S. aureus causes a wide range of severe illnesses in both healthy and immunocompromised indivi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bao G. Vu, Christopher S. Stach, Katarina Kulhankova, Wilmara Salgado-Pabón, Aloysius J. Klingelhutz, Patrick M. Schlievert
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a3fe8697edd444cc870955388df674d3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a3fe8697edd444cc870955388df674d3
record_format	dspace
spelling	oai:doaj.org-article:a3fe8697edd444cc870955388df674d32021-11-15T15:41:34ZChronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes10.1128/mBio.02554-142150-7511https://doaj.org/article/a3fe8697edd444cc870955388df674d32015-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02554-14https://doaj.org/toc/2150-7511ABSTRACT Excessive weight and obesity are associated with the development of diabetes mellitus type 2 (DMII) in humans. They also pose high risks of Staphylococcus aureus colonization and overt infections. S. aureus causes a wide range of severe illnesses in both healthy and immunocompromised individuals. Among S. aureus virulence factors, superantigens are essential for pathogenicity. In this study, we show that rabbits that are chronically exposed to S. aureus superantigen toxic shock syndrome toxin-1 (TSST-1) experience impaired glucose tolerance, systemic inflammation, and elevated endotoxin levels in the bloodstream, all of which are common findings in DMII. Additionally, such DMII-associated findings are also seen through effects of TSST-1 on isolated adipocytes. Collectively, our findings suggest that chronic exposure to S. aureus superantigens facilitates the development of DMII, which may lead to therapeutic targeting of S. aureus and its superantigens. IMPORTANCE Obesity has a strong correlation with type 2 diabetes, in which fatty tissue, containing adipocytes, contributes to the development of the illness through altered metabolism and chronic inflammation. The human microbiome changes in persons with obesity and type 2 diabetes, including increases in Staphylococcus aureus colonization and overt infections. While the microbiome is essential for human wellness, there is little understanding of the role of microbes in obesity or the development of diabetes. Here, we demonstrate that the S. aureus superantigen toxic shock syndrome toxin-1 (TSST-1), an essential exotoxin in pathogenesis, induces inflammation, lipolysis, and insulin resistance in adipocytes both in vitro and in vivo. Chronic stimulation of rabbits with TSST-1 results in impaired systemic glucose tolerance, the hallmark finding in type 2 diabetes in humans, suggesting a role of S. aureus and its superantigens in the progression to type 2 diabetes.Bao G. VuChristopher S. StachKatarina KulhankovaWilmara Salgado-PabónAloysius J. KlingelhutzPatrick M. SchlievertAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 2 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Bao G. Vu Christopher S. Stach Katarina Kulhankova Wilmara Salgado-Pabón Aloysius J. Klingelhutz Patrick M. Schlievert Chronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes
description	ABSTRACT Excessive weight and obesity are associated with the development of diabetes mellitus type 2 (DMII) in humans. They also pose high risks of Staphylococcus aureus colonization and overt infections. S. aureus causes a wide range of severe illnesses in both healthy and immunocompromised individuals. Among S. aureus virulence factors, superantigens are essential for pathogenicity. In this study, we show that rabbits that are chronically exposed to S. aureus superantigen toxic shock syndrome toxin-1 (TSST-1) experience impaired glucose tolerance, systemic inflammation, and elevated endotoxin levels in the bloodstream, all of which are common findings in DMII. Additionally, such DMII-associated findings are also seen through effects of TSST-1 on isolated adipocytes. Collectively, our findings suggest that chronic exposure to S. aureus superantigens facilitates the development of DMII, which may lead to therapeutic targeting of S. aureus and its superantigens. IMPORTANCE Obesity has a strong correlation with type 2 diabetes, in which fatty tissue, containing adipocytes, contributes to the development of the illness through altered metabolism and chronic inflammation. The human microbiome changes in persons with obesity and type 2 diabetes, including increases in Staphylococcus aureus colonization and overt infections. While the microbiome is essential for human wellness, there is little understanding of the role of microbes in obesity or the development of diabetes. Here, we demonstrate that the S. aureus superantigen toxic shock syndrome toxin-1 (TSST-1), an essential exotoxin in pathogenesis, induces inflammation, lipolysis, and insulin resistance in adipocytes both in vitro and in vivo. Chronic stimulation of rabbits with TSST-1 results in impaired systemic glucose tolerance, the hallmark finding in type 2 diabetes in humans, suggesting a role of S. aureus and its superantigens in the progression to type 2 diabetes.
format	article
author	Bao G. Vu Christopher S. Stach Katarina Kulhankova Wilmara Salgado-Pabón Aloysius J. Klingelhutz Patrick M. Schlievert
author_facet	Bao G. Vu Christopher S. Stach Katarina Kulhankova Wilmara Salgado-Pabón Aloysius J. Klingelhutz Patrick M. Schlievert
author_sort	Bao G. Vu
title	Chronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes
title_short	Chronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes
title_full	Chronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes
title_fullStr	Chronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes
title_full_unstemmed	Chronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes
title_sort	chronic superantigen exposure induces systemic inflammation, elevated bloodstream endotoxin, and abnormal glucose tolerance in rabbits: possible role in diabetes
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/a3fe8697edd444cc870955388df674d3
work_keys_str_mv	AT baogvu chronicsuperantigenexposureinducessystemicinflammationelevatedbloodstreamendotoxinandabnormalglucosetoleranceinrabbitspossibleroleindiabetes AT christophersstach chronicsuperantigenexposureinducessystemicinflammationelevatedbloodstreamendotoxinandabnormalglucosetoleranceinrabbitspossibleroleindiabetes AT katarinakulhankova chronicsuperantigenexposureinducessystemicinflammationelevatedbloodstreamendotoxinandabnormalglucosetoleranceinrabbitspossibleroleindiabetes AT wilmarasalgadopabon chronicsuperantigenexposureinducessystemicinflammationelevatedbloodstreamendotoxinandabnormalglucosetoleranceinrabbitspossibleroleindiabetes AT aloysiusjklingelhutz chronicsuperantigenexposureinducessystemicinflammationelevatedbloodstreamendotoxinandabnormalglucosetoleranceinrabbitspossibleroleindiabetes AT patrickmschlievert chronicsuperantigenexposureinducessystemicinflammationelevatedbloodstreamendotoxinandabnormalglucosetoleranceinrabbitspossibleroleindiabetes
_version_	1718427660663652352

Chronic Superantigen Exposure Induces Systemic Inflammation, Elevated Bloodstream Endotoxin, and Abnormal Glucose Tolerance in Rabbits: Possible Role in Diabetes

Ejemplares similares