Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.

Human PUF-A/PUM3 is a RNA and DNA binding protein participating in the nucleolar processing of 7S to 5.8S rRNA. The nucleolar localization of PUF-A redistributes to the nucleoplasm upon the exposure to genotoxic agents in cells. However, little is known regarding the roles of PUF-A in tumor progress...

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Autores principales:	Hung-Wei Lin, Jin-Yu Lee, Nai-Lin Chou, Ting-Wei Shih, Mau-Sun Chang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a400f67bda40476188a07de2769a2575
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spelling	oai:doaj.org-article:a400f67bda40476188a07de2769a25752021-12-02T20:17:49ZPhosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.1932-620310.1371/journal.pone.0256282https://doaj.org/article/a400f67bda40476188a07de2769a25752021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256282https://doaj.org/toc/1932-6203Human PUF-A/PUM3 is a RNA and DNA binding protein participating in the nucleolar processing of 7S to 5.8S rRNA. The nucleolar localization of PUF-A redistributes to the nucleoplasm upon the exposure to genotoxic agents in cells. However, little is known regarding the roles of PUF-A in tumor progression. Phosphoprotein database analysis revealed that Y259 phosphorylation of PUF-A is the most prevalent residue modified. Here, we reported the importance of PUF-A's phosphorylation on Y259 in tumorigenesis. PUF-A gene was knocked out by the Crispr/Cas9 method in human cervix epithelial HeLa cells. Loss of PUF-A in HeLa cells resulted in reduced clonogenic and lower transwell invasion capacity. Introduction of PUF-AY259F to PUF-A deficient HeLa cells was unable to restore colony formation. In addition, the unphosphorylated mutant of PUF-A, PUF-AY259F, attenuated PUF-A protein stability. Our results suggest the important role of Y259 phosphorylation of PUF-A in cell proliferation.Hung-Wei LinJin-Yu LeeNai-Lin ChouTing-Wei ShihMau-Sun ChangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256282 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hung-Wei Lin Jin-Yu Lee Nai-Lin Chou Ting-Wei Shih Mau-Sun Chang Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.
description	Human PUF-A/PUM3 is a RNA and DNA binding protein participating in the nucleolar processing of 7S to 5.8S rRNA. The nucleolar localization of PUF-A redistributes to the nucleoplasm upon the exposure to genotoxic agents in cells. However, little is known regarding the roles of PUF-A in tumor progression. Phosphoprotein database analysis revealed that Y259 phosphorylation of PUF-A is the most prevalent residue modified. Here, we reported the importance of PUF-A's phosphorylation on Y259 in tumorigenesis. PUF-A gene was knocked out by the Crispr/Cas9 method in human cervix epithelial HeLa cells. Loss of PUF-A in HeLa cells resulted in reduced clonogenic and lower transwell invasion capacity. Introduction of PUF-AY259F to PUF-A deficient HeLa cells was unable to restore colony formation. In addition, the unphosphorylated mutant of PUF-A, PUF-AY259F, attenuated PUF-A protein stability. Our results suggest the important role of Y259 phosphorylation of PUF-A in cell proliferation.
format	article
author	Hung-Wei Lin Jin-Yu Lee Nai-Lin Chou Ting-Wei Shih Mau-Sun Chang
author_facet	Hung-Wei Lin Jin-Yu Lee Nai-Lin Chou Ting-Wei Shih Mau-Sun Chang
author_sort	Hung-Wei Lin
title	Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.
title_short	Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.
title_full	Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.
title_fullStr	Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.
title_full_unstemmed	Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.
title_sort	phosphorylation of puf-a/pum3 on y259 modulates puf-a stability and cell proliferation.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a400f67bda40476188a07de2769a2575
work_keys_str_mv	AT hungweilin phosphorylationofpufapum3ony259modulatespufastabilityandcellproliferation AT jinyulee phosphorylationofpufapum3ony259modulatespufastabilityandcellproliferation AT nailinchou phosphorylationofpufapum3ony259modulatespufastabilityandcellproliferation AT tingweishih phosphorylationofpufapum3ony259modulatespufastabilityandcellproliferation AT mausunchang phosphorylationofpufapum3ony259modulatespufastabilityandcellproliferation
_version_	1718374363462369280

Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.

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