Reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space

Abstract Telocytes are a recently described stromal cell type widely distributed in various organs including the female and male reproductive systems. This study was aimed to investigate for the first time the existence, distribution and characteristics of telocytes in normal human testis by an inte...

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Autores principales:	Mirca Marini, Irene Rosa, Daniele Guasti, Mauro Gacci, Eleonora Sgambati, Lidia Ibba-Manneschi, Mirko Manetti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Telocytes (TCs) Human Testis Stromal Space Telopodes Podomeres Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a4041e20385441f39467bbb7e2504e88
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spelling	oai:doaj.org-article:a4041e20385441f39467bbb7e2504e882021-12-02T15:08:06ZReappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space10.1038/s41598-018-33126-22045-2322https://doaj.org/article/a4041e20385441f39467bbb7e2504e882018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33126-2https://doaj.org/toc/2045-2322Abstract Telocytes are a recently described stromal cell type widely distributed in various organs including the female and male reproductive systems. This study was aimed to investigate for the first time the existence, distribution and characteristics of telocytes in normal human testis by an integrated morphological approach (immunohistochemistry, immunofluorescence and transmission electron microscopy). We found that telocytes displaying typical long and moniliform prolongations and coexpressing CD34 and PDGFRα formed networks in the outer layer of peritubular tissue and around Leydig cells and vessels in the intertubular stroma. Testicular telocytes were immunophenotypically negative for CD31, c-kit/CD117 as well as α-SMA, thus making them clearly distinguishable from myoid cells/myofibroblasts located in the inner layer of peritubular tissue. Transmission electron microscopy confirmed the presence of cells ultrastructurally identifiable as telocytes (i.e. cells with telopodes alternating podomers and podoms) in the aforementioned locations. Intercellular contacts between neighboring telocytes and telopodes were observed throughout the testicular stromal compartment. Telopodes intimately surrounded and often established close contacts with peritubular myoid cells/myofibroblasts, Leydig cells and vessels. Extracellular vesicles were also frequently detected near telopodes. In summary, we demonstrated that telocytes are a previously neglected stromal component of human testis with potential implications in tissue homeostasis deserving further investigation.Mirca MariniIrene RosaDaniele GuastiMauro GacciEleonora SgambatiLidia Ibba-ManneschiMirko ManettiNature PortfolioarticleTelocytes (TCs)Human TestisStromal SpaceTelopodesPodomeresMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Telocytes (TCs) Human Testis Stromal Space Telopodes Podomeres Medicine R Science Q
spellingShingle	Telocytes (TCs) Human Testis Stromal Space Telopodes Podomeres Medicine R Science Q Mirca Marini Irene Rosa Daniele Guasti Mauro Gacci Eleonora Sgambati Lidia Ibba-Manneschi Mirko Manetti Reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space
description	Abstract Telocytes are a recently described stromal cell type widely distributed in various organs including the female and male reproductive systems. This study was aimed to investigate for the first time the existence, distribution and characteristics of telocytes in normal human testis by an integrated morphological approach (immunohistochemistry, immunofluorescence and transmission electron microscopy). We found that telocytes displaying typical long and moniliform prolongations and coexpressing CD34 and PDGFRα formed networks in the outer layer of peritubular tissue and around Leydig cells and vessels in the intertubular stroma. Testicular telocytes were immunophenotypically negative for CD31, c-kit/CD117 as well as α-SMA, thus making them clearly distinguishable from myoid cells/myofibroblasts located in the inner layer of peritubular tissue. Transmission electron microscopy confirmed the presence of cells ultrastructurally identifiable as telocytes (i.e. cells with telopodes alternating podomers and podoms) in the aforementioned locations. Intercellular contacts between neighboring telocytes and telopodes were observed throughout the testicular stromal compartment. Telopodes intimately surrounded and often established close contacts with peritubular myoid cells/myofibroblasts, Leydig cells and vessels. Extracellular vesicles were also frequently detected near telopodes. In summary, we demonstrated that telocytes are a previously neglected stromal component of human testis with potential implications in tissue homeostasis deserving further investigation.
format	article
author	Mirca Marini Irene Rosa Daniele Guasti Mauro Gacci Eleonora Sgambati Lidia Ibba-Manneschi Mirko Manetti
author_facet	Mirca Marini Irene Rosa Daniele Guasti Mauro Gacci Eleonora Sgambati Lidia Ibba-Manneschi Mirko Manetti
author_sort	Mirca Marini
title	Reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space
title_short	Reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space
title_full	Reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space
title_fullStr	Reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space
title_full_unstemmed	Reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space
title_sort	reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a4041e20385441f39467bbb7e2504e88
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Reappraising the microscopic anatomy of human testis: identification of telocyte networks in the peritubular and intertubular stromal space

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