Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.

Caveolae are specialized domains of the plasma membrane, which play key roles in signaling, endocytosis and mechanosensing. Using total internal reflection fluorescent microscopy (TIRF-M), we observe that the exocyst subunit Exo70 forms punctuate structures at the plasma membrane and partially local...

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Autores principales:	Maud Hertzog, Pedro Monteiro, Gaëlle Le Dez, Philippe Chavrier
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a40b0c2623b6464ba5a402c5f8d96f0c
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spelling	oai:doaj.org-article:a40b0c2623b6464ba5a402c5f8d96f0c2021-11-18T08:03:29ZExo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.1932-620310.1371/journal.pone.0052627https://doaj.org/article/a40b0c2623b6464ba5a402c5f8d96f0c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23300727/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Caveolae are specialized domains of the plasma membrane, which play key roles in signaling, endocytosis and mechanosensing. Using total internal reflection fluorescent microscopy (TIRF-M), we observe that the exocyst subunit Exo70 forms punctuate structures at the plasma membrane and partially localizes with caveolin-1, the main component of caveolae. Upon cell detachment, we found that Exo70 accumulates with caveolin-1-positive vesicular structures. Upon cell re-adhesion, caveolin-1 traffics back to the plasma membrane in a multistep process involving microtubules and actin cytoskeleton. In addition, silencing of Exo70 redirects caveolin-1 to focal adhesions identified by markers such as α5 integrin or vinculin. Based on these findings, we conclude that Exo70 is involved in caveolin-1 recycling to the plasma membrane during re-adhesion of the cells to the substratum.Maud HertzogPedro MonteiroGaëlle Le DezPhilippe ChavrierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52627 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maud Hertzog Pedro Monteiro Gaëlle Le Dez Philippe Chavrier Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.
description	Caveolae are specialized domains of the plasma membrane, which play key roles in signaling, endocytosis and mechanosensing. Using total internal reflection fluorescent microscopy (TIRF-M), we observe that the exocyst subunit Exo70 forms punctuate structures at the plasma membrane and partially localizes with caveolin-1, the main component of caveolae. Upon cell detachment, we found that Exo70 accumulates with caveolin-1-positive vesicular structures. Upon cell re-adhesion, caveolin-1 traffics back to the plasma membrane in a multistep process involving microtubules and actin cytoskeleton. In addition, silencing of Exo70 redirects caveolin-1 to focal adhesions identified by markers such as α5 integrin or vinculin. Based on these findings, we conclude that Exo70 is involved in caveolin-1 recycling to the plasma membrane during re-adhesion of the cells to the substratum.
format	article
author	Maud Hertzog Pedro Monteiro Gaëlle Le Dez Philippe Chavrier
author_facet	Maud Hertzog Pedro Monteiro Gaëlle Le Dez Philippe Chavrier
author_sort	Maud Hertzog
title	Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.
title_short	Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.
title_full	Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.
title_fullStr	Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.
title_full_unstemmed	Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.
title_sort	exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a40b0c2623b6464ba5a402c5f8d96f0c
work_keys_str_mv	AT maudhertzog exo70subunitoftheexocystcomplexisinvolvedinadhesiondependenttraffickingofcaveolin1 AT pedromonteiro exo70subunitoftheexocystcomplexisinvolvedinadhesiondependenttraffickingofcaveolin1 AT gaelleledez exo70subunitoftheexocystcomplexisinvolvedinadhesiondependenttraffickingofcaveolin1 AT philippechavrier exo70subunitoftheexocystcomplexisinvolvedinadhesiondependenttraffickingofcaveolin1
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Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.

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