Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents

This first comprehensive report from Punjab province of India relates to patho-physiological alterations alongwith morpho-molecular characterisation and risk assessment of natural infections of Hymenolepis diminuta and Hymenolepis nana in 291commensal rodents including house rat, Rattus rattus (n=20...

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Autores principales:	Brar S. K., Singla N., Singla L. D.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	cestode histopathology hymenolepis molecular parasite rodents Agriculture (General) S1-972 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a40d815d74174bcabf60f6dda2ea5a86
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spelling	oai:doaj.org-article:a40d815d74174bcabf60f6dda2ea5a862021-12-05T14:11:07ZComparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents1336-908310.2478/helm-2021-0027https://doaj.org/article/a40d815d74174bcabf60f6dda2ea5a862021-09-01T00:00:00Zhttps://doi.org/10.2478/helm-2021-0027https://doaj.org/toc/1336-9083This first comprehensive report from Punjab province of India relates to patho-physiological alterations alongwith morpho-molecular characterisation and risk assessment of natural infections of Hymenolepis diminuta and Hymenolepis nana in 291commensal rodents including house rat, Rattus rattus (n=201) and lesser bandicoot rat, Bandicota bangalensis (n=90). Small intestine of 53.61 and 64.95 % rats was found infected with H. diminuta and H. nana, respectively with a concurrent infection rate of 50.86 %. There was no association between male and female rats and H. diminuta and H. nana infections (ᵡ2 = 0.016 and 0.08, respectively, d.f.= 1, P>0.05), while the host age had significant effect on prevalence of H. diminuta and H. nana (ᵡ2 = 28.12 and 7.18, respectively, d.f.= 1, P≤0.05) infection. Examination of faecal samples and intestinal contents revealed globular shaped eggs of H. diminuta without polar filaments (76.50 ± 3.01μm x 67.62 ± 2.42 μm), while smaller sized oval eggs of H. nana were with 4 – 8 polar filaments (47.87 ± 1.95 μm x 36.12 ± 3.05 μm). Cestode infection caused enteritis, sloughing of intestinal mucosa, necrosis of villi and inflammatory reaction with infiltration of mononuclear cells in the mucosa and submucosa. Morphometric identification of the adult cestodes recovered from the intestinal lumen was confirmed by molecular characterisation based on nuclear ITS-2 loci which showed a single band of 269 bp and 242 bp for H. diminuta and H. nana, respectively. Pairwise alignment of the ITS-2 regions showed 99.46 % similarity with sequences of H. diminuta from USA and 100 % similarity with sequences of H. nana from Slovakia, Kosice.Brar S. K.Singla N.Singla L. D.SciendoarticlecestodehistopathologyhymenolepismolecularparasiterodentsAgriculture (General)S1-972Medicine (General)R5-920ENHelminthologia, Vol 58, Iss 3, Pp 248-262 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cestode histopathology hymenolepis molecular parasite rodents Agriculture (General) S1-972 Medicine (General) R5-920
spellingShingle	cestode histopathology hymenolepis molecular parasite rodents Agriculture (General) S1-972 Medicine (General) R5-920 Brar S. K. Singla N. Singla L. D. Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents
description	This first comprehensive report from Punjab province of India relates to patho-physiological alterations alongwith morpho-molecular characterisation and risk assessment of natural infections of Hymenolepis diminuta and Hymenolepis nana in 291commensal rodents including house rat, Rattus rattus (n=201) and lesser bandicoot rat, Bandicota bangalensis (n=90). Small intestine of 53.61 and 64.95 % rats was found infected with H. diminuta and H. nana, respectively with a concurrent infection rate of 50.86 %. There was no association between male and female rats and H. diminuta and H. nana infections (ᵡ2 = 0.016 and 0.08, respectively, d.f.= 1, P>0.05), while the host age had significant effect on prevalence of H. diminuta and H. nana (ᵡ2 = 28.12 and 7.18, respectively, d.f.= 1, P≤0.05) infection. Examination of faecal samples and intestinal contents revealed globular shaped eggs of H. diminuta without polar filaments (76.50 ± 3.01μm x 67.62 ± 2.42 μm), while smaller sized oval eggs of H. nana were with 4 – 8 polar filaments (47.87 ± 1.95 μm x 36.12 ± 3.05 μm). Cestode infection caused enteritis, sloughing of intestinal mucosa, necrosis of villi and inflammatory reaction with infiltration of mononuclear cells in the mucosa and submucosa. Morphometric identification of the adult cestodes recovered from the intestinal lumen was confirmed by molecular characterisation based on nuclear ITS-2 loci which showed a single band of 269 bp and 242 bp for H. diminuta and H. nana, respectively. Pairwise alignment of the ITS-2 regions showed 99.46 % similarity with sequences of H. diminuta from USA and 100 % similarity with sequences of H. nana from Slovakia, Kosice.
format	article
author	Brar S. K. Singla N. Singla L. D.
author_facet	Brar S. K. Singla N. Singla L. D.
author_sort	Brar S. K.
title	Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents
title_short	Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents
title_full	Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents
title_fullStr	Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents
title_full_unstemmed	Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents
title_sort	comparative comprehensive analysis on natural infections of hymenolepis diminuta and hymenolepis nana in commensal rodents
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/a40d815d74174bcabf60f6dda2ea5a86
work_keys_str_mv	AT brarsk comparativecomprehensiveanalysisonnaturalinfectionsofhymenolepisdiminutaandhymenolepisnanaincommensalrodents AT singlan comparativecomprehensiveanalysisonnaturalinfectionsofhymenolepisdiminutaandhymenolepisnanaincommensalrodents AT singlald comparativecomprehensiveanalysisonnaturalinfectionsofhymenolepisdiminutaandhymenolepisnanaincommensalrodents
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Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents

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